GDP-mannose 4,6-dehydratase (P0AC88)

Identification
Name:GDP-mannose 4,6-dehydratase
Synonyms:
  • GDP-D-mannose dehydratase
Gene Name:gmd
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H(2)O
Cellular Location:Not Available
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism II PW000887
  • colanic acid building blocks biosynthesis PW000951
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Guanosine diphosphate mannose ↔ 1.0GDP-4-Dehydro-6-deoxy-D-mannose + 1.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanosine diphosphate mannose → 1.0Water + 1.0GDP-4-dehydro-6-deoxy-α-D-mannose
ReactionCard
1.0Thumb → 1.0GDP-4-Dehydro-6-L-deoxygalactose+1.0Thumb
1.0Guanosine diphosphate mannose → 1.0GDP-4-Dehydro-6-L-deoxygalactose + 1.0GDP-4-Dehydro-6-L-deoxygalactose
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Guanosine diphosphate mannose ↔ 1.0GDP-4-Dehydro-6-deoxy-D-mannose + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01346GDP-4-Dehydro-6-deoxy-D-mannoseMetaboCard
ECMDB24242GDP-4-dehydro-6-deoxy-α-D-mannoseMetaboCard
ECMDB01391GDP-4-Dehydro-6-L-deoxygalactoseMetaboCard
ECMDB01163Guanosine diphosphate mannoseMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
intracellular
Function
binding
carbon-oxygen lyase activity
catalytic activity
coenzyme binding
cofactor binding
GDP-mannose 4,6-dehydratase activity
hydro-lyase activity
lyase activity
Process
alcohol metabolic process
cellular metabolic process
GDP-mannose metabolic process
hexose metabolic process
mannose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Blattner:b2053
Gene OrientationCounterclockwise
Centisome Percentage:45.81
Left Sequence End2125217
Right Sequence End2126338
Gene Sequence:
>1122 bp
ATGTCAAAAGTCGCTCTCATCACCGGTGTAACCGGACAAGACGGTTCTTACCTGGCAGAG
TTTCTGCTGGAAAAAGGTTACGAGGTGCATGGTATTAAGCGTCGCGCATCGTCATTCAAC
ACCGAGCGCGTGGATCACATTTATCAGGATCCGCACACCTGCAACCCGAAATTCCATCTG
CATTATGGCGACCTGAGTGATACCTCTAACCTGACGCGCATTTTGCGTGAAGTACAGCCG
GATGAAGTGTACAACCTGGGCGCAATGAGCCACGTTGCGGTCTCTTTTGAGTCACCAGAA
TATACCGCTGACGTCGACGCGATGGGTACGCTGCGCCTGCTGGAGGCGATCCGCTTCCTC
GGTCTGGAAAAGAAAACTCGTTTCTATCAGGCTTCCACCTCTGAACTGTATGGTCTGGTG
CAGGAAATTCCGCAGAAAGAGACCACGCCGTTCTACCCGCGATCTCCGTATGCGGTCGCC
AAACTGTACGCCTACTGGATCACCGTTAACTACCGTGAATCCTACGGCATGTACGCCTGT
AACGGAATTCTCTTCAACCATGAATCCCCGCGCCGCGGCGAAACCTTCGTTACCCGCAAA
ATCACCCGCGCAATCGCCAACATCGCCCAGGGGCTGGAGTCGTGCCTGTACCTCGGCAAT
ATGGATTCCCTGCGTGACTGGGGCCACGCCAAAGACTACGTAAAAATGCAGTGGATGATG
CTGCAGCAGGAACAGCCGGAAGATTTCGTTATCGCGACCGGCGTTCAGTACTCCGTGCGT
CAGTTCGTGGAAATGGCGGCAGCACAGCTGGGCATCAAACTGCGCTTTGAAGGCACGGGC
GTTGAAGAGAAGGGCATTGTGGTTTCCGTCACCGGGCATGACGCGCCGGGCGTTAAACCG
GGTGATGTGATTATCGCTGTTGACCCGCGTTACTTCCGTCCGGCTGAAGTTGAAACGCTG
CTCGGCGACCCGACCAAAGCGCACGAAAAACTGGGCTGGAAACCGGAAATCACCCTCAGA
GAGATGGTGTCTGAAATGGTGGCTAATGACCTCGAAGCGGCGAAAAAACACTCTCTGCTG
AAATCTCACGGCTACGACGTGGCGATCGCGCTGGAGTCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:373
Protein Molecular Weight:42047
Protein Theoretical pI:6
PDB File:1DB3
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>GDP-mannose 4,6-dehydratase
MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERVDHIYQDPHTCNPKFHL
HYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYTADVDAMGTLRLLEAIRFL
GLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWITVNYRESYGMYAC
NGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYLGNMDSLRDWGHAKDYVKMQWMM
LQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRFEGTGVEEKGIVVSVTGHDAPGVKP
GDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWKPEITLREMVSEMVANDLEAAKKHSLL
KSHGYDVAIALES
References
External Links:
ResourceLink
Uniprot ID:P0AC88
Uniprot Name:GM4D_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736759
PDB ID:1DB3
Ecogene ID:EG11787
Ecocyc:EG11787
ColiBase:b2053
Kegg Gene:b2053
EchoBASE ID:EB1735
CCDB:GM4D_ECOLI
BacMap:16129993
General Reference:
  • Aoyama, K., Haase, A. M., Reeves, P. R. (1994). "Evidence for effect of random genetic drift on G+C content after lateral transfer of fucose pathway genes to Escherichia coli K-12." Mol Biol Evol 11:829-838. Pubmed: 7815923
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Somoza, J. R., Menon, S., Schmidt, H., Joseph-McCarthy, D., Dessen, A., Stahl, M. L., Somers, W. S., Sullivan, F. X. (2000). "Structural and kinetic analysis of Escherichia coli GDP-mannose 4,6 dehydratase provides insights into the enzyme's catalytic mechanism and regulation by GDP-fucose." Structure 8:123-135. Pubmed: 10673432
  • Stevenson, G., Andrianopoulos, K., Hobbs, M., Reeves, P. R. (1996). "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid." J Bacteriol 178:4885-4893. Pubmed: 8759852
  • Sturla, L., Bisso, A., Zanardi, D., Benatti, U., De Flora, A., Tonetti, M. (1997). "Expression, purification and characterization of GDP-D-mannose 4,6-dehydratase from Escherichia coli." FEBS Lett 412:126-130. Pubmed: 9257704