DNA polymerase III subunit theta (P0ABS8)

Identification
Name:DNA polymerase III subunit theta
Synonyms:Not Available
Gene Name:holE
Enzyme Class:
Biological Properties
General Function:Involved in DNA binding
Specific Function:The exact function of the theta subunit is unknown
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dATP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dGTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dCTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0Thymidine 5'-triphosphate + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Deoxynucleoside triphosphate+1.0DNA ↔ 1.0Thumb
1.0Deoxynucleoside triphosphate + 1.0DNA ↔ 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01532dATPMetaboCard
ECMDB00998dCTPMetaboCard
ECMDB01440dGTPMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
DNA binding
DNA polymerase activity
DNA-directed DNA polymerase activity
nucleic acid binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b1842
Gene OrientationClockwise
Centisome Percentage:41.45
Left Sequence End1923132
Right Sequence End1923362
Gene Sequence:
>231 bp
ATGCGCCGCTGGATTTCACAAAATAATATCAGGCTCCCTCGTGGAGCCTTTTTTATATCT
GCCTTATTTTTCTTCAACGCTGTATGTATAGTAAGCGATAACCTGTTGATTATTGAATCT
TTCGGGGAGATGGCTTATAACATTTCTTACCTGACCAGGGTACCGGGAACCAACACCTTA
CTGGCGTGTTGCTGTCTTTTAAGACCAGAAGAGGTTAACAGTGAATATTGA
Protein Properties
Pfam Domain Function:
Protein Residues:76
Protein Molecular Weight:8846
Protein Theoretical pI:10
PDB File:1DU2
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA polymerase III subunit theta
MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQPEHLRSWFRERLIAHR
LASVNLSRLPYEPKLK
References
External Links:
ResourceLink
Uniprot ID:P0ABS8
Uniprot Name:HOLE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062550
PDB ID:1DU2
Ecogene ID:EG11505
Ecocyc:EG11505
ColiBase:b1842
Kegg Gene:b1842
EchoBASE ID:EB1468
CCDB:HOLE_ECOLI
BacMap:16129795
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Carter, J. R., Franden, M. A., Aebersold, R., Kim, D. R., McHenry, C. S. (1993). "Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme." Nucleic Acids Res 21:3281-3286. Pubmed: 8341603
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • O'Donnell, M. (1992). "Accessory protein function in the DNA polymerase III holoenzyme from E. coli." Bioessays 14:105-111. Pubmed: 1575709
  • Studwell-Vaughan, P. S., O'Donnell, M. (1993). "DNA polymerase III accessory proteins. V. Theta encoded by holE." J Biol Chem 268:11785-11791. Pubmed: 8505306