Biotin carboxyl carrier protein of acetyl-CoA carboxylase (P0ABD8)

Identification
Name:Biotin carboxyl carrier protein of acetyl-CoA carboxylase
Synonyms:
  • BCCP
Gene Name:accB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in acetyl-CoA carboxylase activity
Specific Function:This protein is a component of the acetyl coenzyme A carboxylase complex; first, biotin carboxylase catalyzes the carboxylation of the carrier protein and then the transcarboxylase transfers the carboxyl group to form malonyl-CoA
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Adenosine triphosphate + 1.0Hydrogen carbonate ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Malonyl-CoA + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Acetyl-CoA + 1.0Hydrogen carbonate ↔ 1.0ADP + 1.0Phosphate + 1.0Malonyl-CoA
ReactionCard
1.0Thumb+1.0Carboxybiotin-carboxyl-carrier protein ↔ 1.0Thumb+1.0Holo-[carboxylase]
1.0Acetyl-CoA + 1.0Carboxybiotin-carboxyl-carrier protein ↔ 1.0Malonyl-CoA + 1.0Holo-[carboxylase]
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb+1.0Malonyl-CoA+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Hydrogen carbonate + 1.0Adenosine triphosphate → 1.0Adenosine diphosphate + 1.0Phosphate + 1.0Hydrogen ion + 1.0Malonyl-CoA + 1.0ADP + 1.0Malonyl-CoA
ReactionCard
1.0a biotinylated [BCCP dimer]+1.0Thumb+1.0Thumb → 1.0carboxylated-biotinylated [BCCP dimer]+1.0Thumb+1.0Thumb+1.0Thumb
1.0a biotinylated [BCCP dimer] + 1.0Adenosine triphosphate + 1.0Hydrogen carbonate → 1.0carboxylated-biotinylated [BCCP dimer] + 1.0Hydrogen ion + 1.0ADP + 1.0Phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Adenosine triphosphate + 1.0Hydrogen carbonate ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Malonyl-CoA + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01206Acetyl-CoAMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00595Hydrogen carbonateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01175Malonyl-CoAMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Component
acetyl-CoA carboxylase complex
macromolecular complex
protein complex
Function
acetyl-CoA carboxylase activity
binding
biotin binding
catalytic activity
CoA carboxylase activity
ligase activity
ligase activity, forming carbon-carbon bonds
vitamin binding
Process
carboxylic acid metabolic process
cellular metabolic process
fatty acid biosynthetic process
fatty acid metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
Gene Properties
Blattner:b3255
Gene OrientationClockwise
Centisome Percentage:73.36
Left Sequence End3403458
Right Sequence End3403928
Gene Sequence:
>471 bp
ATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGCCATTTTGCTC
AGCAAAGCTAAAAAAGATCTCTCATTTGCCGAGATTGCCGACGGCACCGGTCTGGCAGAA
GCCTTTGTAACCGCGGCTTTGCTGGGTCAGCAGGCGCTTCCTGCCGACGCCGCCCGCCTG
GTCGGGGCGAAGCTGGATCTCGACGAAGACTCCATTCTACTGTTGCAGATGATTCCACTG
CGTGGCTGCATTGATGACCGTATTCCAACTGACCCAACGATGTATCGTTTCTATGAAATG
TTGCAGGTGTACGGTACAACCCTGAAAGCGTTGGTTCATGAGAAATTTGGCGATGGCATT
ATTAGCGCGATTAACTTCAAACTCGACGTTAAGAAAGTGGCGGACCCGGAAGGTGGCGAA
CGTGCGGTCATCACCTTAGATGGTAAATATCTGCCGACCAAACCGTTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:156
Protein Molecular Weight:16687
Protein Theoretical pI:4
PDB File:1A6X
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Biotin carboxyl carrier protein of acetyl-CoA carboxylase
MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVMQQAYAAPMMQQPAQSN
AAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAFIEVGQKVNVGDTLCIVEA
MKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE
References
External Links:
ResourceLink
Uniprot ID:P0ABD8
Uniprot Name:BCCP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674482
PDB ID:1A6X
Ecogene ID:EG10275
Ecocyc:EG10275
ColiBase:b3255
Kegg Gene:b3255
EchoBASE ID:EB0271
CCDB:BCCP_ECOLI
BacMap:16131143
General Reference:
  • Alix, J. H. (1989). "A rapid procedure for cloning genes from lambda libraries by complementation of E. coli defective mutants: application to the fabE region of the E. coli chromosome." DNA 8:779-789. Pubmed: 2575489
  • Athappilly, F. K., Hendrickson, W. A. (1995). "Structure of the biotinyl domain of acetyl-coenzyme A carboxylase determined by MAD phasing." Structure 3:1407-1419. Pubmed: 8747466
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kondo, H., Shiratsuchi, K., Yoshimoto, T., Masuda, T., Kitazono, A., Tsuru, D., Anai, M., Sekiguchi, M., Tanabe, T. (1991). "Acetyl-CoA carboxylase from Escherichia coli: gene organization and nucleotide sequence of the biotin carboxylase subunit." Proc Natl Acad Sci U S A 88:9730-9733. Pubmed: 1682920
  • Li, S. J., Cronan, J. E. Jr (1992). "The gene encoding the biotin carboxylase subunit of Escherichia coli acetyl-CoA carboxylase." J Biol Chem 267:855-863. Pubmed: 1370469
  • Li, S. J., Cronan, J. E. Jr (1993). "Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis." J Bacteriol 175:332-340. Pubmed: 7678242
  • Muramatsu, S., Mizuno, T. (1989). "Nucleotide sequence of the fabE gene and flanking regions containing a bent DNA sequence of Escherichia coli." Nucleic Acids Res 17:3982. Pubmed: 2660106
  • Roberts, E. L., Shu, N., Howard, M. J., Broadhurst, R. W., Chapman-Smith, A., Wallace, J. C., Morris, T., Cronan, J. E. Jr, Perham, R. N. (1999). "Solution structures of apo and holo biotinyl domains from acetyl coenzyme A carboxylase of Escherichia coli determined by triple-resonance nuclear magnetic resonance spectroscopy." Biochemistry 38:5045-5053. Pubmed: 10213607
  • Sutton, M. R., Fall, R. R., Nervi, A. M., Alberts, A. W., Vagelos, P. R., Bradshaw, R. A. (1977). "Amino acid sequence of Escherichia coli biotin carboxyl carrier protein (9100)." J Biol Chem 252:3934-3940. Pubmed: 324999
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841
  • Yao, X., Wei, D., Soden, C. Jr, Summers, M. F., Beckett, D. (1997). "Structure of the carboxy-terminal fragment of the apo-biotin carboxyl carrier subunit of Escherichia coli acetyl-CoA carboxylase." Biochemistry 36:15089-15100. Pubmed: 9398236