Branched-chain-amino-acid aminotransferase (P0AB80)

Identification
Name:Branched-chain-amino-acid aminotransferase
Synonyms:
  • BCAT
  • Transaminase B
Gene Name:ilvE
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Acts on leucine, isoleucine and valine
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0alpha-Ketoglutarate + 1.0L-Phenylalanine ↔ 1.0L-Glutamate + 1.0Phenylpyruvic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0alpha-Ketoglutarate + 1.0L-Isoleucine ↔ 1.03-Methyl-2-oxovaleric acid + 1.0L-Glutamate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0alpha-Ketoglutarate + 1.0L-Valine ↔ 1.0alpha-Ketoisovaleric acid + 1.0L-Glutamate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.04-Methyl-2-oxopentanoate+1.0Thumb+1.0Thumb
1.0L-Leucine + 1.0alpha-Ketoglutarate ↔ 1.04-Methyl-2-oxopentanoate + 1.0L-Glutamate + 1.0Ketoleucine
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0L-Glutamic acid+1.0Thumb → 1.0Thumb+1.0L-Valine+1.0Thumb
1.0a-Ketoisovaleric acid + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Valine + 1.0L-Valine
ReactionCard
1.0Thumb+1.0L-Glutamic acid+1.0Thumb → 1.0Thumb+1.0L-Valine+1.0Thumb
1.0Isovaleric acid + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Valine + 1.0L-Valine
ReactionCard
1.03-Methyl-2-oxovaleric acid+1.0L-Glutamic acid+1.0Thumb+1.0Thumb → 1.0Thumb+1.0L-Valine+1.0Thumb
1.03-Methyl-2-oxovaleric acid + 1.0L-Glutamic acid + 1.03-Methyl-2-oxovaleric acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Valine + 1.0L-Valine
ReactionCard
1.0Thumb+1.0L-Glutamic acid+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Ketoleucine + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Leucine
ReactionCard
1.0Thumb+1.0L-Glutamic acid+1.0Thumb → 1.0Thumb+1.0L-Phenylalanine+1.0Thumb
1.0Phenylpyruvic acid + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Phenylalanine + 1.0L-Phenylalanine
ReactionCard
1.03-Methyl-2-oxovaleric acid+1.0L-Glutamic acid+1.0Thumb+1.0Thumb → 1.0Thumb+1.0L-Isoleucine+1.0Thumb
1.03-Methyl-2-oxovaleric acid + 1.0L-Glutamic acid + 1.03-Methyl-2-oxovaleric acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0L-Isoleucine + 1.0L-Isoleucine
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Leucine + 1.0Oxoglutaric acid ↔ 1.0Ketoleucine + 1.0L-Glutamate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Ketoleucine + 1.0L-Glutamate → 1.0alpha-Ketoglutarate + 1.0L-Leucine
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Leucine + 1.0Oxoglutaric acid → 1.0Ketoleucine + 1.0L-Glutamate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Valine + 1.0Oxoglutaric acid → 1.0a-Ketoisovaleric acid + 1.0L-Glutamate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB040912-Keto-3-methyl-valerateMetaboCard
ECMDB004913-Methyl-2-oxovaleric acidMetaboCard
ECMDB00019a-Ketoisovaleric acidMetaboCard
ECMDB02812alpha-KetoglutarateMetaboCard
ECMDB04092alpha-Ketoisovaleric acidMetaboCard
ECMDB00718Isovaleric acidMetaboCard
ECMDB00695KetoleucineMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB00172L-IsoleucineMetaboCard
ECMDB00687L-LeucineMetaboCard
ECMDB00159L-PhenylalanineMetaboCard
ECMDB00883L-ValineMetaboCard
ECMDB04123Oxoglutaric acidMetaboCard
ECMDB00205Phenylpyruvic acidMetaboCard
GO Classification:
Function
branched-chain-amino-acid transaminase activity
catalytic activity
transaminase activity
transferase activity
transferase activity, transferring nitrogenous groups
Process
branched chain family amino acid metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b3770
Gene OrientationClockwise
Centisome Percentage:85.15
Left Sequence End3950507
Right Sequence End3951436
Gene Sequence:
>930 bp
ATGATTCAGCAAGGAGATCTCATGCCGCAGTCCGCGTTGTTCACGGGAATCATTCCCCCT
GTCTCCACCATTTTTACCGCCGACGGCCAGCTCGATAAGCCGGGCACCGCCGCGCTGATC
GACGATCTGATCAAAGCAGGCGTTGACGGCCTGTTCTTCCTGGGCAGCGGTGGCGAGTTC
TCCCAGCTCGGCGCCGAAGAGCGTAAAGCCATTGCCCGCTTTGCTATCGATCATGTCGAT
CGTCGCGTGCCGGTGCTGATCGGCACCGGCGGCACCAACGCCCGGGAAACCATCGAACTC
AGCCAGCACGCGCAGCAGGCGGGCGCGGACGGCATCGTGGTGATCAACCCCTACTACTGG
AAAGTGTCGGAAGCGAACCTGATCCGCTATTTCGAGCAGGTGGCCGACAGCGTCACGCTG
CCGGTGATGCTCTATAACTTCCCGGCGCTGACCGGGCAGGATCTGACTCCGGCGCTGGTG
AAAACCCTCGCCGACTCGCGCAGCAATATTATCGGCATCAAAGACACCATCGACTCCGTC
GCCCACCTGCGCAGCATGATCCATACCGTCAAAGGTGCCCATCCGCACTTCACCGTGCTC
TGCGGCTACGACGATCATCTGTTCAATACCCTGCTGCTCGGCGGCGACGGGGCGATATCG
GCGAGCGGCAACTTTGCCCCGCAGGTGTCGGTGAATCTTCTGAAAGCCTGGCGCGACGGG
GACGTGGCGAAAGCGGCCGGGTATCATCAGACCTTGCTGCAAATTCCGCAGATGTATCAG
CTGGATACGCCGTTTGTGAACGTGATTAAAGAGGCGATCGTGCTCTGCGGTCGTCCTGTC
TCCACGCACGTGCTGCCGCCCGCCTCGCCGCTGGACGAGCCGCGCAAGGCGCAGCTGAAA
ACCCTGCTGCAACAGCTCAAGCTTTGCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:309
Protein Molecular Weight:34093
Protein Theoretical pI:6
PDB File:1IYE
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Branched-chain-amino-acid aminotransferase
MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYDSHKGPVVFRHREHMQR
LHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIFVGDVGMGVNPPAGYSTDV
IIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAKAGGNYLSSLLVGSEARRHGY
QEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSALPGITRDAIIKLAKELGIEVREQ
VLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEGRCGPVTKRIQQAFFGLFTGETEDK
WGWLDQVNQ
References
External Links:
ResourceLink
Uniprot ID:P0AB80
Uniprot Name:ILVE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674412
PDB ID:1IYE
Ecogene ID:EG10497
Ecocyc:EG10497
ColiBase:b3770
Kegg Gene:b3770
EchoBASE ID:EB0492
CCDB:ILVE_ECOLI
BacMap:49176403
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Inoue, K., Kuramitsu, S., Aki, K., Watanabe, Y., Takagi, T., Nishigai, M., Ikai, A., Kagamiyama, H. (1988). "Branched-chain amino acid aminotransferase of Escherichia coli: overproduction and properties." J Biochem 104:777-784. Pubmed: 3069843
  • Kuramitsu, S., Ogawa, T., Ogawa, H., Kagamiyama, H. (1985). "Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence." J Biochem 97:993-999. Pubmed: 3897211
  • Lawther, R. P., Nichols, B., Zurawski, G., Hatfield, G. W. (1979). "The nucleotide sequence preceding and including the beginning of the ilvE gene of the ilvGEDA operon of Escherichia coli K12." Nucleic Acids Res 7:2289-2301. Pubmed: 392469
  • Lawther, R. P., Wek, R. C., Lopes, J. M., Pereira, R., Taillon, B. E., Hatfield, G. W. (1987). "The complete nucleotide sequence of the ilvGMEDA operon of Escherichia coli K-12." Nucleic Acids Res 15:2137-2155. Pubmed: 3550695
  • Okada, K., Hirotsu, K., Sato, M., Hayashi, H., Kagamiyama, H. (1997). "Three-dimensional structure of Escherichia coli branched-chain amino acid aminotransferase at 2.5 A resolution." J Biochem 121:637-641. Pubmed: 9163511
  • Pagel, J. M., Winkelman, J. W., Adams, C. W., Hatfield, G. W. (1992). "DNA topology-mediated regulation of transcription initiation from the tandem promoters of the ilvGMEDA operon of Escherichia coli." J Mol Biol 224:919-935. Pubmed: 1569580
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293