ECMDB: D-tagatose-1,6-bisphosphate aldolase subunit kbaY (P0AB74)

Identification

Name:

D-tagatose-1,6-bisphosphate aldolase subunit kbaY

Synonyms:

TBPA
TagBP aldolase
D-tagatose-bisphosphate aldolase class II
Ketose 1,6-bisphosphate aldolase class II
Tagatose-bisphosphate aldolase

Gene Name:

kbaY

Enzyme Class:

4.1.2.40

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Catalytic subunit of the tagatose-1,6-bisphosphate aldolase kbaYZ, which catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6- bisphosphate (TBP). Requires kbaZ subunit for full activity and stability

Cellular Location:

Not Available

SMPDB Pathways:

Galactitol and galactonate degradation PW000820
Galactose metabolism PW000821

KEGG Pathways:

Galactose metabolism ec00052
Metabolic pathways eco01100

SMPDB Reactions:

1.0D-tagatofuranose 1,6-bisphosphate

→

1.0

1.0D-Glyceraldehyde 3-phosphate

1.0

1.0D-tagatofuranose 1,6-bisphosphate → 1.0Dihydroxyacetone phosphate + 1.0D-Glyceraldehyde 3-phosphate + 1.0D-Glyceraldehyde 3-phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01112	D-Glyceraldehyde 3-phosphate	MetaboCard
ECMDB06872	D-Tagatose 1,6-bisphosphate	MetaboCard
ECMDB01473	Dihydroxyacetone phosphate	MetaboCard

GO Classification:

Function
aldehyde-lyase activity
binding
carbon-carbon lyase activity
catalytic activity
cation binding
fructose-bisphosphate aldolase activity
ion binding
lyase activity
metal ion binding
transition metal ion binding
zinc ion binding
Process
alcohol metabolic process
carbohydrate metabolic process
disaccharide metabolic process
glucose catabolic process
glucose metabolic process
glycolysis
hexose metabolic process
lactose catabolic process
lactose catabolic process via tagatose-6-phosphate
lactose metabolic process
metabolic process
monosaccharide metabolic process
oligosaccharide metabolic process
primary metabolic process
small molecule metabolic process

Gene Properties

Blattner:

b3137

Gene Orientation

Clockwise

Centisome Percentage:

70.72

Left Sequence End

3281165

Right Sequence End

3282025

Gene Sequence:

>861 bp
ATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCG
ATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACC
GTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGT
CCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACA
ATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGACGCATCCTGGTTGCCCGCATTTTCT
CCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTG
GATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGATT
GAGGCCAGCCTGACCGTGCTGGCAGAGATGCAACAACAAGGCCTGGTAAAACATATTGGC
CTGAGCAACGTCACACCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGT
GTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCC
CACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCG
TCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCG
TGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCAT
TTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTG
GATGGTATTTCGCGAGAATAA

Protein Properties

Pfam Domain Function:

F_bP_aldolase (PF01116 )

Protein Residues:

286

Protein Molecular Weight:

31293

Protein Theoretical pI:

PDB File:

1GVF

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>D-tagatose-1,6-bisphosphate aldolase subunit kbaY
MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSPVILAGTPGTFKHIALE
EIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAMIDGSHFPFAENVKLVKSV
VDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEAKRFVELTGVDSLAVAIGTAH
GLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFVRRTIELGVTKVNVATELKIAFA
GAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKINVCGSANRISA

References

External Links:

Resource	Link
Uniprot ID:	P0AB74
Uniprot Name:	KBAY_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1742295
PDB ID:	1GVF
Ecogene ID:	EG12768
Ecocyc:	EG12768
ColiBase:	b3137
Kegg Gene:	b3137
EchoBASE ID:	EB2621
CCDB:	KBAY_ECOLI
BacMap:	16131029

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Brinkkotter, A., Shakeri-Garakani, A., Lengeler, J. W. (2002). "Two class II D-tagatose-bisphosphate aldolases from enteric bacteria." Arch Microbiol 177:410-419. Pubmed: 11976750
Hall, D. R., Bond, C. S., Leonard, G. A., Watt, C. I., Berry, A., Hunter, W. N. (2002). "Structure of tagatose-1,6-bisphosphate aldolase. Insight into chiral discrimination, mechanism, and specificity of class II aldolases." J Biol Chem 277:22018-22024. Pubmed: 11940603
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Reizer, J., Ramseier, T. M., Reizer, A., Charbit, A., Saier, M. H. Jr (1996). "Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Microbiology 142 ( Pt 2):231-250. Pubmed: 8932697
Zgiby, S. M., Thomson, G. J., Qamar, S., Berry, A. (2000). "Exploring substrate binding and discrimination in fructose1, 6-bisphosphate and tagatose 1,6-bisphosphate aldolases." Eur J Biochem 267:1858-1868. Pubmed: 10712619