D-tagatose-1,6-bisphosphate aldolase subunit kbaY (P0AB74)

Identification
Name:D-tagatose-1,6-bisphosphate aldolase subunit kbaY
Synonyms:
  • TBPA
  • TagBP aldolase
  • D-tagatose-bisphosphate aldolase class II
  • Ketose 1,6-bisphosphate aldolase class II
  • Tagatose-bisphosphate aldolase
Gene Name:kbaY
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalytic subunit of the tagatose-1,6-bisphosphate aldolase kbaYZ, which catalyzes the reversible aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to produce tagatose 1,6- bisphosphate (TBP). Requires kbaZ subunit for full activity and stability
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
SMPDB Reactions:
1.0D-tagatofuranose 1,6-bisphosphate → 1.0Thumb+1.0D-Glyceraldehyde 3-phosphate+1.0Thumb
1.0D-tagatofuranose 1,6-bisphosphate → 1.0Dihydroxyacetone phosphate + 1.0D-Glyceraldehyde 3-phosphate + 1.0D-Glyceraldehyde 3-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01112D-Glyceraldehyde 3-phosphateMetaboCard
ECMDB06872D-Tagatose 1,6-bisphosphateMetaboCard
ECMDB01473Dihydroxyacetone phosphateMetaboCard
GO Classification:
Function
aldehyde-lyase activity
binding
carbon-carbon lyase activity
catalytic activity
cation binding
fructose-bisphosphate aldolase activity
ion binding
lyase activity
metal ion binding
transition metal ion binding
zinc ion binding
Process
alcohol metabolic process
carbohydrate metabolic process
disaccharide metabolic process
glucose catabolic process
glucose metabolic process
glycolysis
hexose metabolic process
lactose catabolic process
lactose catabolic process via tagatose-6-phosphate
lactose metabolic process
metabolic process
monosaccharide metabolic process
oligosaccharide metabolic process
primary metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3137
Gene OrientationClockwise
Centisome Percentage:70.72
Left Sequence End3281165
Right Sequence End3282025
Gene Sequence:
>861 bp
ATGAGCAGCAATACATTTACTCTCGGTACAAAATCCGTTAACCGTCTTGGTTATGGCGCG
ATGCAACTGGCAGGTCCTGGAGTTTTTGGCCCCCCACGAGATCGCCACGTCGCTATAACC
GTGCTGCGTGAGGCGCTGGCATTGGGCGTCAATCATATTGATACCAGCGACTTTTATGGT
CCGCACGTCACCAATCAGATTATCCGCGAAGCGCTTTATCCTTACTCTGACGACCTGACA
ATTGTCACTAAAATTGGTGCGCGGCGTGGAGAGGACGCATCCTGGTTGCCCGCATTTTCT
CCGGCAGAGCTGCAAAAAGCGGTGCACGATAATCTACGTAATCTCGGGCTGGACGTGCTG
GATGTGGTTAACCTGCGCGTTATGATGGGGGATGGTCATGGCCCAGCGGAAGGATCGATT
GAGGCCAGCCTGACCGTGCTGGCAGAGATGCAACAACAAGGCCTGGTAAAACATATTGGC
CTGAGCAACGTCACACCGACGCAGGTTGCAGAGGCGCGCAAGATTGCCGAAATTGTCTGT
GTGCAAAACGAATACAACATCGCGCACCGTGCTGATGATGCAATGATTGATGCTTTGGCC
CACGATGGCATTGCCTACGTGCCGTTCTTCCCGCTCGGGGGCTTTACACCGCTGCAATCG
TCCACACTTTCCGATGTTGCTGCGAGCCTGGGTGCAACACCAATGCAGGTGGCGCTGGCG
TGGCTGTTACAGCGTTCACCGAATATTTTGCTGATCCCAGGGACGTCTTCGGTTGCGCAT
TTACGGGAGAATATGGCTGCTGAAAAATTGCATCTTTCTGAGGAAGTGTTGTCTACGTTG
GATGGTATTTCGCGAGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:286
Protein Molecular Weight:31293
Protein Theoretical pI:5
PDB File:1GVF
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>D-tagatose-1,6-bisphosphate aldolase subunit kbaY
MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSPVILAGTPGTFKHIALE
EIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAMIDGSHFPFAENVKLVKSV
VDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEAKRFVELTGVDSLAVAIGTAH
GLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFVRRTIELGVTKVNVATELKIAFA
GAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKINVCGSANRISA
References
External Links:
ResourceLink
Uniprot ID:P0AB74
Uniprot Name:KBAY_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742295
PDB ID:1GVF
Ecogene ID:EG12768
Ecocyc:EG12768
ColiBase:b3137
Kegg Gene:b3137
EchoBASE ID:EB2621
CCDB:KBAY_ECOLI
BacMap:16131029
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Brinkkotter, A., Shakeri-Garakani, A., Lengeler, J. W. (2002). "Two class II D-tagatose-bisphosphate aldolases from enteric bacteria." Arch Microbiol 177:410-419. Pubmed: 11976750
  • Hall, D. R., Bond, C. S., Leonard, G. A., Watt, C. I., Berry, A., Hunter, W. N. (2002). "Structure of tagatose-1,6-bisphosphate aldolase. Insight into chiral discrimination, mechanism, and specificity of class II aldolases." J Biol Chem 277:22018-22024. Pubmed: 11940603
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Reizer, J., Ramseier, T. M., Reizer, A., Charbit, A., Saier, M. H. Jr (1996). "Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli." Microbiology 142 ( Pt 2):231-250. Pubmed: 8932697
  • Zgiby, S. M., Thomson, G. J., Qamar, S., Berry, A. (2000). "Exploring substrate binding and discrimination in fructose1, 6-bisphosphate and tagatose 1,6-bisphosphate aldolases." Eur J Biochem 267:1858-1868. Pubmed: 10712619