Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit (P0AAM1)

Identification
Name:Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit
Synonyms:Not Available
Gene Name:hyaC
Enzyme Class:Not Available
Biological Properties
General Function:Involved in electron carrier activity
Specific Function:Probable b-type cytochrome
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:Not Available
SMPDB Reactions:
1.0Thumb+1.0Electron+2.0Thumb+1.0menaquinone-8 → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Electron + 2.0Hydrogen ion + 1.0menaquinone-8 → 1.0Menaquinol 8 + 1.0Hydrogen ion
ReactionCard
Complex Reactions:
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Ubiquinone-8 → 1.0Ubiquinol-8 + 2.0Hydrogen ion
ReactionCard
1.0Thumb+2.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
1.02-Demethylmenaquinone 8 + 2.0Hydrogen ion + 1.0Hydrogen (gas) → 1.02-Demethylmenaquinol 8 + 2.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb+1.0Menaquinone 8 → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Menaquinone 8 → 1.0Menaquinol 8 + 2.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
binding
catalytic activity
cation binding
electron carrier activity
ion binding
iron ion binding
metal ion binding
oxidoreductase activity
transition metal ion binding
Process
cellular metabolic process
electron transport chain
establishment of localization
generation of precursor metabolites and energy
metabolic process
respiratory electron transport chain
transport
Gene Properties
Blattner:b0974
Gene OrientationClockwise
Centisome Percentage:22.29
Left Sequence End1034289
Right Sequence End1034996
Gene Sequence:
>708 bp
ATGGACATGGAGTCGCAAAAAATATTGTTTGCACTTTCCACTCCGATGGAAATACGCAAT
GAATGCTGTTTACCTTCGCATTCATCACCCAAAATGTATTTAGGTACATGCTTTTTTGAC
CTTTCTTCCTCATGGGGAATTGATGACCGTGATGACCTGCTGCGCACAATTCACCGAATG
ATCGATAACGGTCATGCCGCTCGGTTGGCAGGGTTTTATCACCGCTGGTTTCGTTATTCG
CCATGTGAATGGCGTGACTATCTTGCTGAACTAAATGAGCAAGGTCAGGCTTATGCGCAA
TTTGTCGCCAGCACCGCCGAATGCTGTGGCGAAGGGGGAATCAAAGCCTGGGATTATGTC
CGGATGGGTTTTTTAAGCCGAATGGGCGTACTTAATAACTGGTTGAGCGAAGAAGAAAGT
CTATGGATCCAGTCGCGCATTCATCTACGGGCTCTACGTTATTATAGTAACTGGCGGCAA
TACTTTGCCGGTTATACCTTTGGTCGGCAATATTGGCAGTCTCCCGAAGATGATCATCTG
CCTTTATTACGCGAATTCTTAGCGCGTAAAGAATACGACGATTCCGGCAATGATATGTTT
TATCAATTATTTGCCAGTGATGATGCGTATTACCCTACCTTGTCCTGGCAACCATTGGCT
TACTATTCTGCATGCCCGGAAACGCTTAAGGATATGAGCGACCTATGA
Protein Properties
Pfam Domain Function:
Protein Residues:235
Protein Molecular Weight:27597
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 20-40
  • 64-84
  • 131-151
  • 186-203
Protein Sequence:
>Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit
MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLPSVSGEATYLFYMGYIR
LIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWWQGVWYEIRWYLFLAKRPS
ADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIFAPFRYVVEFFYWTGGNSMDI
HSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMVNGYRSHKFGKISNKERS
References
External Links:
ResourceLink
Uniprot ID:P0AAM1
Uniprot Name:CYBH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062260
Ecogene ID:EG10470
Ecocyc:EG10470
ColiBase:b0974
Kegg Gene:b0974
EchoBASE ID:EB0465
CCDB:CYBH_ECOLI
BacMap:16128940
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Menon, N. K., Robbins, J., Peck, H. D. Jr, Chatelus, C. Y., Choi, E. S., Przybyla, A. E. (1990). "Cloning and sequencing of a putative Escherichia coli [NiFe] hydrogenase-1 operon containing six open reading frames." J Bacteriol 172:1969-1977. Pubmed: 2180913
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232