Hydrogenase-2 operon protein hybA (P0AAJ8)

Identification
Name:Hydrogenase-2 operon protein hybA
Synonyms:Not Available
Gene Name:hybA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in electron carrier activity
Specific Function:Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Ubiquinone-8 → 1.0Ubiquinol-8 + 2.0Hydrogen ion
ReactionCard
1.0Thumb+2.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
1.02-Demethylmenaquinone 8 + 2.0Hydrogen ion + 1.0Hydrogen (gas) → 1.02-Demethylmenaquinol 8 + 2.0Hydrogen ion
ReactionCard
2.0Thumb+1.0Thumb+1.0Menaquinone 8 → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Menaquinone 8 → 1.0Menaquinol 8 + 2.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211542-Demethylmenaquinol 8MetaboCard
ECMDB211552-Demethylmenaquinone 8MetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21245Menaquinol 8MetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Function
binding
electron carrier activity
iron-sulfur cluster binding
metal cluster binding
Gene Properties
Blattner:b2996
Gene OrientationCounterclockwise
Centisome Percentage:67.72
Left Sequence End3142176
Right Sequence End3143162
Gene Sequence:
>987 bp
ATGATCGAAAAAATCTGGTCTGGTGAATCCCCTTTGTGGCGGCTATTGCTGCCACTCTCC
TGGTTGTATGGCCTGGTGAGTGGCGCGATCCGTCTTTGCTATAAACTAAAACTGAAGCGC
GCCTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGA
AAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGG
GTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGAT
ACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCT
GTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGAT
GTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAA
ATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCA
ATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTC
CCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACC
GGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCAT
CCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCG
CTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAA
ACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGG
TGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAA
CTAACCTTGCTGGCTTCTGGCAACTAG
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:328
Protein Molecular Weight:36003
Protein Theoretical pI:7
Signaling Regions:
  • 1-27
Transmembrane Regions:
  • None
Protein Sequence:
>Hydrogenase-2 operon protein hybA
MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDSTLCVGCQACVTKCQDI
NFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEENGYAYIKKQCMHCVDPNC
VSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVPKYDYNNPFGALHKCELCNQK
GVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRLALKPGSEYHYPRQTLKSGDTYL
HTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPKLDDLSTGARSENIQHTLYKGMMLP
LAVLAGLTVLVRRNTKNDHHDGGDDHES
References
External Links:
ResourceLink
Uniprot ID:P0AAJ8
Uniprot Name:HYBA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062487
Ecogene ID:EG11799
Ecocyc:EG11799
ColiBase:b2996
Kegg Gene:b2996
EchoBASE ID:EB1747
CCDB:HYBA_ECOLI
BacMap:16130896
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Menon, N. K., Chatelus, C. Y., Dervartanian, M., Wendt, J. C., Shanmugam, K. T., Peck, H. D. Jr, Przybyla, A. E. (1994). "Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2." J Bacteriol 176:4416-4423. Pubmed: 8021226
  • Tullman-Ercek, D., DeLisa, M. P., Kawarasaki, Y., Iranpour, P., Ribnicky, B., Palmer, T., Georgiou, G. (2007). "Export pathway selectivity of Escherichia coli twin arginine translocation signal peptides." J Biol Chem 282:8309-8316. Pubmed: 17218314