3-oxoacyl-[acyl-carrier-protein] synthase 2 (P0AAI5)

• 3-oxoacyl-[acyl-carrier-protein] synthase II
• Beta-ketoacyl-ACP synthase II
• KAS II

• 2.3.1.179

• Biotin metabolism PW000762
• Fatty acid biosynthesis PW000900
• Fatty acid metabolism PW000796
• fatty acid elongation -- saturated PW000798

• Biotin metabolism ec00780
• Fatty acid biosynthesis ec00061
• Fatty acid metabolism ec00071
• Metabolic pathways eco01100

>1242 bp
ATGAACAAAACGCTAATCGCCGCAGCTGTGGCAGGGATAGTTTTACTCGCTTCAAACGCT
CAGGCACAAACCGTACCGGAAGGCTATCAGCTACAGCAAGTGCTCATGATGAGCCGCCAT
AACTTACGTGCGCCGCTGGCGAACAATGGCAGTGTGCTGGAGCAGTCGACGCCGAATAAA
TGGCCAGAATGGGACGTCCCCGGTGGGCAACTCACCACCAAAGGTGGCGTGCTCGAAGTG
TATATGGGCCATTACATGCGTGAATGGCTGGCAGAGCAGGGGATGGTGAAATCGGGGGAA
TGCCCGCCGCCGTACACCGTTTATGCCTATGCCAATAGTCTGCAACGTACCGTTGCGACC
GCACAGTTCTTTATTACCGGCGCATTCCCGGGGTGTGATATTCCTGTGCATCACCAGGAA
AAAATGGGCACTATGGACCCAACCTTTAACCCGGTGATCACCGATGATTCCGCCGCATTC
AGTGAACAGGCGGTGGCGGCAATGGAGAAAGAGCTCAGCAAACTCCAGCTTACCGACAGC
TACCAGCTACTGGAAAAAATCGTTAACTATAAAGATTCCCCTGCCTGTAAAGAGAAACAA
CAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGT
GTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTAC
GAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAG
GTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGCCTGTTTACCTCACCGGAAGTGGCG
CGCAATGTTGCGAAACCGCTGGTCAGTTATATCGACAAAGCTCTGGTCACCGATCGCACC
AGCGCACCGAAAATTACAGTGTTGGTTGGGCACGACTCCAACATTGCCTCTCTGTTAACG
GCGCTGGATTTCAAACCGTATCAGTTGCATGACCAGAACGAACGCACGCCGATTGGCGGC
AAAATCGTTTTCCAGCGTTGGCATGACAGCAAAGCCAATCGCGATTTGATGAAAATTGAA
TATGTGTATCAGAGTGCGGAACAGTTACGTAATGCCGATGCGTTAACCCTGCAGGCACCT
GCGCAGCGTGTGACGCTGGAATTAAGCGGTTGCCCGATAGACGCTGATGGTTTCTGCCCG
ATGGATAAGTTTGATAGCGTGTTGAATGAAGCGGTGAAATAA

• ketoacyl-synt (PF00109 )
• Ketoacyl-synt_C (PF02801 )

• None

• None

>3-oxoacyl-[acyl-carrier-protein] synthase 2
MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTSAYATKFAGLVKDFNCE
DIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIGAAIGSGIGGLGLIEENHT
SLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISIATACTSGVHNIGHAARIIAY
GDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQAASRPWDKERDGFVLGDGAGMLV
LEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPENGAGAALAMANALRDAGIEASQIGY
VNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSSTKSMTGHLLGAAGAVESIYSILALRD
QAVPPTINLDNPDEGCDLDFVPHEARQVSGMEYTLCNSFGFGGTNGSLIFKKI

• Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
• Edwards, P., Nelsen, J. S., Metz, J. G., Dehesh, K. (1997). "Cloning of the fabF gene in an expression vector and in vitro characterization of recombinant fabF and fabB encoded enzymes from Escherichia coli." FEBS Lett 402:62-66. Pubmed: 9013860
• Garwin, J. L., Klages, A. L., Cronan, J. E. Jr (1980). "Beta-ketoacyl-acyl carrier protein synthase II of Escherichia coli. Evidence for function in the thermal regulation of fatty acid synthesis." J Biol Chem 255:3263-3265. Pubmed: 6988423
• Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
• Huang, W., Jia, J., Edwards, P., Dehesh, K., Schneider, G., Lindqvist, Y. (1998). "Crystal structure of beta-ketoacyl-acyl carrier protein synthase II from E.coli reveals the molecular architecture of condensing enzymes." EMBO J 17:1183-1191. Pubmed: 9482715
• Jackowski, S., Rock, C. O. (1987). "Altered molecular form of acyl carrier protein associated with beta-ketoacyl-acyl carrier protein synthase II (fabF) mutants." J Bacteriol 169:1469-1473. Pubmed: 3549687
• Magnuson, K., Carey, M. R., Cronan, J. E. Jr (1995). "The putative fabJ gene of Escherichia coli fatty acid synthesis is the fabF gene." J Bacteriol 177:3593-3595. Pubmed: 7768872
• Moche, M., Schneider, G., Edwards, P., Dehesh, K., Lindqvist, Y. (1999). "Structure of the complex between the antibiotic cerulenin and its target, beta-ketoacyl-acyl carrier protein synthase." J Biol Chem 274:6031-6034. Pubmed: 10037680
• Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
• Siggaard-Andersen, M., Wissenbach, M., Chuck, J. A., Svendsen, I., Olsen, J. G., von Wettstein-Knowles, P. (1994). "The fabJ-encoded beta-ketoacyl-[acyl carrier protein] synthase IV from Escherichia coli is sensitive to cerulenin and specific for short-chain substrates." Proc Natl Acad Sci U S A 91:11027-11031. Pubmed: 7972002