Phosphate import ATP-binding protein pstB (P0AAH0)

Identification
Name:Phosphate import ATP-binding protein pstB
Synonyms:
  • ABC phosphate transporter
  • Phosphate-transporting ATPase
Gene Name:pstB
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:Part of the ABC transporter complex pstSACB involved in phosphate import. Responsible for energy coupling to the transport system
Cellular Location:Cell inner membrane; Peripheral membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+2.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Phosphate → 1.0ADP + 1.0Hydrogen ion + 2.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0phosphate(Out) → 1.0Thumb+1.0Thumb+1.0phosphate(In)
1.0Adenosine triphosphate + 1.0Water + 1.0phosphate(Out) → 1.0ADP + 1.0Inorganic phosphate + 1.0phosphate(In)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
anion transmembrane transporter activity
ATP binding
ATPase activity
binding
catalytic activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
inorganic anion transmembrane transporter activity
inorganic phosphate transmembrane transporter activity
ion transmembrane transporter activity
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
phosphate transmembrane transporter activity
phosphate transmembrane-transporting ATPase activity
purine nucleoside binding
pyrophosphatase activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
phosphate transport
transport
Gene Properties
Blattner:b3725
Gene OrientationCounterclockwise
Centisome Percentage:84.18
Left Sequence End3905616
Right Sequence End3906389
Gene Sequence:
>774 bp
ATGGCTCGTAAATGGTTGAACTTGTTTGCCGGGGCGGCACTCTCTTTCGCTGTTGCTGGC
AATGCACTGGCAGATGAAGGGAAAATCACGGTGTTCGCCGCCGCATCACTGACTAACGCA
ATGCAGGACATTGCTACGCAGTTTAAAAAAGAGAAAGGCGTGGATGTGGTTTCTTCTTTC
GCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATT
TCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAGCGATCGATACAGCTACG
CGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAA
GATTTCACCATCGACAGCAAAACCAACTGGACTTCACTGCTGAATGGCGGTCGCCTGGCG
GTTGGCGATCCGGAACATGTTCCCGCTGGCATTTATGCAAAAGAAGCACTGCAAAAACTG
GGCGCATGGGATACGCTCTCTCCGAAACTGGCCCCAGCGGAAGATGTTCGTGGGGCGCTG
GCGCTGGTCGAACGTAACGAAGCGCCTCTGGGCATTGTCTACGGTTCTGACGCAGTTGCC
AGCAAAGGGGTAAAAGTGGTTGCCACCTTCCCGGAAGATTCACATAAAAAAGTGGAATAT
CCGGTTGCTGTTGTGGAAGGGCATAACAATGCGACAGTGAAAGCATTTTATGATTATCTG
AAGGGACCGCAGGCAGCGGAAATCTTTAAACGTTACGGATTTACAATCAAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:257
Protein Molecular Weight:29027
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phosphate import ATP-binding protein pstB
MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGPSGCGKSTLLRTFNKMF
ELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTPFPMSIYDNIAFGVRLFEK
LSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQRLCIARGIAIRPEVLLLDEP
CSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARCSDHTAFMYLGELIEFSNTDDLF
TKPAKKQTEDYITGRYG
References
External Links:
ResourceLink
Uniprot ID:P0AAH0
Uniprot Name:PSTB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651348
Ecogene ID:EG10783
Ecocyc:EG10783
ColiBase:b3725
Kegg Gene:b3725
EchoBASE ID:EB0776
CCDB:PSTB_ECOLI
BacMap:16131593
General Reference:
  • Amemura, M., Makino, K., Shinagawa, H., Kobayashi, A., Nakata, A. (1985). "Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli." J Mol Biol 184:241-250. Pubmed: 2993631
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Chan, F. Y., Torriani, A. (1996). "PstB protein of the phosphate-specific transport system of Escherichia coli is an ATPase." J Bacteriol 178:3974-3977. Pubmed: 8682808
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Surin, B. P., Rosenberg, H., Cox, G. B. (1985). "Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships." J Bacteriol 161:189-198. Pubmed: 3881386