ECMDB

Identification

Name:

Probable cadaverine/lysine antiporter

Synonyms:

Not Available

Gene Name:

cadB

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in amino acid transmembrane transporter activity

Specific Function:

Probable cadaverine/lysine antiporter or part of it

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

Lysine Degradation I PW000772
inner membrane transport PW000786

KEGG Pathways:

Not Available

Transports:

Transport References:

Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831

Metabolites:

ECMDB ID	Name	View
ECMDB02322	Cadaverine	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00182	L-Lysine	MetaboCard

GO Classification:

Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
active transmembrane transporter activity
amine transmembrane transporter activity
amino acid transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
amine transport
amino acid transport
establishment of localization
transmembrane transport
transport

Gene Properties

Blattner:

b4132

Gene Orientation

Counterclockwise

Centisome Percentage:

93.90

Left Sequence End

4356720

Right Sequence End

4358054

Gene Sequence:

>1335 bp
ATGACTGTAACGACTTTTTCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAG
GATAAAGGTTTCACTCGCCCGACCGCCATTCAGGCTGCCGCCATTCCGCCTGCGCTCGAT
GGCCGTGATGTACTCGGTTCTGCGCCGACAGGCACCGGTAAAACGGCGGCGTATCTGCTG
CCAGCGTTGCAGCACCTGCTCGATTTCCCGCGTAAGAAATCCGGTCCGCCGCGTATTTTG
ATCCTCACCCCAACTCGCGAGCTGGCGATGCAGGTGTCCGATCATGCCCGCGAACTGGCG
AAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCG
GAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATAC
ATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGAC
CGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGG
CGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCC
GAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAA
AAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTG
CATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGT
GTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGT
GAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAAC
GTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTC
TTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCG
CGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTG
GGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGC
CCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCT
AAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCAT
CGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAA
ACGACAGAAGAGTAA

Protein Properties

Pfam Domain Function:

AA_permease (PF00324 )

Protein Residues:

444

Protein Molecular Weight:

46665

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

7-27
35-55
68-88
95-115
123-143
149-169
193-213
222-242
250-270
273-293
323-343
354-374
384-404
405-425

Protein Sequence:

>Probable cadaverine/lysine antiporter
MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWIISIIGAMSLAYVYARL
ATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVP
AGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVMTAIVGWHWFDAATYAANWNT
ADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQ
VLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRA
ANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSAGGKASDLFGELTGIAVL
LTMLPYFYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIIL
MFYARKMHERQSHSMDNHTASNAH

References

External Links:

Resource	Link
Uniprot ID:	P0AAE8
Uniprot Name:	CADB_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85675467
Ecogene ID:	EG10132
Ecocyc:	EG10132
ColiBase:	b4132
Kegg Gene:	b4132
EchoBASE ID:	EB0130
CCDB:	CADB_ECOLI
BacMap:	16131958

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Meng, S. Y., Bennett, G. N. (1992). "Nucleotide sequence of the Escherichia coli cad operon: a system for neutralization of low extracellular pH." J Bacteriol 174:2659-2669. Pubmed: 1556085
Watson, N., Dunyak, D. S., Rosey, E. L., Slonczewski, J. L., Olson, E. R. (1992). "Identification of elements involved in transcriptional regulation of the Escherichia coli cad operon by external pH." J Bacteriol 174:530-540. Pubmed: 1370290

Probable cadaverine/lysine antiporter (P0AAE8)