Tryptophan-specific transport protein (P0AAD2)

Identification
Name:Tryptophan-specific transport protein
Synonyms:
  • Tryptophan permease
Gene Name:mtr
Enzyme Class:Not Available
Biological Properties
General Function:Involved in amino acid transmembrane transporter activity
Specific Function:Involved in transporting tryptophan across the cytoplasmic membrane
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB00738IndoleMetaboCard
ECMDB00929L-TryptophanMetaboCard
GO Classification:
Component
cell part
integral to membrane
integral to plasma membrane
intrinsic to membrane
membrane
membrane part
Function
active transmembrane transporter activity
amine transmembrane transporter activity
amino acid transmembrane transporter activity
aromatic amino acid transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
amine transport
amino acid transport
aromatic amino acid transport
establishment of localization
transport
Gene Properties
Blattner:b3161
Gene OrientationCounterclockwise
Centisome Percentage:71.18
Left Sequence End3302595
Right Sequence End3303839
Gene Sequence:
>1245 bp
ATGCGTTTATCTCTCCCTCGCCTGAAAATGCCGCGCCTGCCAGGATTCAGTATCCTGGTC
TGGATCTCCACGGCGCTAAAGGGCTGGGTGATGGGCTCGCGGGAAAAAGATACCGACAGC
CTGATCATGTACGATCGCACCTTACTGTGGCTGACCTTCGGCCTCGCGGCGATTGGCTTT
ATCATGGTGACCTCGGCGTCAATGCCCATAGGGCAACGCTTAACCAACGATCCGTTCTTC
TTCGCGAAGCGTGATGGTGTCTATCTGATTTTGGCGTTTATTCTGGCGATCATTACGCTG
CGTCTGCCGATGGAGTTCTGGCAACGCTACAGTGCCACGATGCTGCTCGGATCTATCATC
CTGCTGATGATCGTCCTGGTAGTGGGTAGCTCGGTTAAAGGGGCATCGCGTTGGATCGAT
CTCGGTTTGCTGCGTATCCAGCCTGCGGAGCTGACAAAACTGTCGCTGTTTTGCTATATC
GCCAACTATCTGGTGCGTAAAGGCGACGAAGTACGTAATAACCTGCGCGGCTTCCTGAAA
CCGATGGGCGTGATTCTGGTGTTGGCAGTGTTACTGCTGGCACAGCCAGACCTTGGTACG
GTGGTGGTGTTGTTTGTGACTACGCTGGCGATGTTGTTCCTGGCGGGAGCGAAATTGTGG
CAGTTCATTGCCATTATCGGTATGGGCATTTCAGCGGTTGTGTTGCTGATACTCGCCGAA
CCGTACCGTATCCGCCGTGTTACCGCATTCTGGAACCCGTGGGAAGATCCCTTTGGCAGC
GGCTATCAGTTAACGCAATCGCTGATGGCGTTTGGTCGCGGCGAACTTTGGGGGCAAGGT
TTAGGTAACTCGGTACAAAAACTGGAGTATCTGCCGGAAGCGCACACTGACTTTATTTTC
GCCATTATCGGCGAAGAACTGGGGTATGTCGGTGTGGTGCTGGCACTTTTAATGGTATTC
TTCGTCGCTTTTCGCGCGATGTCGATTGGCCGTAAAGCATTAGAAATTGACCACCGTTTT
TCCGGTTTTCTCGCCTGTTCTATTGGCATCTGGTTTAGCTTCCAGGCGCTGGTTAACGTA
GGCGCGGCGGCGGGGATGTTACCGACCAAAGGTCTGACATTGCCGCTGATCAGTTACGGT
GGTTCGAGCTTACTGATTATGTCGACAGCCATCATGATGCTGTTGCGTATTGATTATGAA
ACGCGTCTGGAGAAAGCGCAGGCGTTTGTACGAGGTTCACGATGA
Protein Properties
Pfam Domain Function:
Protein Residues:414
Protein Molecular Weight:44332
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 11-34
  • 39-61
  • 87-109
  • 123-145
  • 152-174
  • 189-210
  • 228-251
  • 282-303
  • 322-343
  • 344-365
  • 387-409
Protein Sequence:
>Tryptophan-specific transport protein
MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWSMAALIFTWFCMLHSGL
MILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYILTYAYISASGSILHHTFAE
MSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAKVITFFLTFGSLLGHVQPATL
FNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYYGKDPKTIVKCLVYGTLMALALY
TIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGVLNSRSLDLLLVVFSNFAVASSFLG
VTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPVVGGLLFPNGFLYAIGYAGLAATIWAA
IVPALLARASRKRFGSPKFRVWGGKPMIALILVFGVGNALVHILSSFNLLPVYQ
References
External Links:
ResourceLink
Uniprot ID:P0AAD2
Uniprot Name:MTR_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321970
Ecogene ID:EG10617
Ecocyc:EG10617
ColiBase:b3161
Kegg Gene:b3161
EchoBASE ID:EB0612
CCDB:MTR_ECOLI
BacMap:16131053
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Heatwole, V. M., Somerville, R. L. (1991). "Cloning, nucleotide sequence, and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli K-12." J Bacteriol 173:108-115. Pubmed: 1987112
  • Heatwole, V. M., Somerville, R. L. (1991). "The tryptophan-specific permease gene, mtr, is differentially regulated by the tryptophan and tyrosine repressors in Escherichia coli K-12." J Bacteriol 173:3601-3604. Pubmed: 1904443
  • Sarsero, J. P., Pittard, A. J. (1995). "Membrane topology analysis of Escherichia coli K-12 Mtr permease by alkaline phosphatase and beta-galactosidase fusions." J Bacteriol 177:297-306. Pubmed: 7814318
  • Sarsero, J. P., Wookey, P. J., Pittard, A. J. (1991). "Regulation of expression of the Escherichia coli K-12 mtr gene by TyrR protein and Trp repressor." J Bacteriol 173:4133-4143. Pubmed: 2061290