3-octaprenyl-4-hydroxybenzoate carboxy-lyase (P0AAB4)

Identification
Name:3-octaprenyl-4-hydroxybenzoate carboxy-lyase
Synonyms:
  • Polyprenyl p-hydroxybenzoate decarboxylase
Gene Name:ubiD
Enzyme Class:
Biological Properties
General Function:Involved in carboxy-lyase activity
Specific Function:Catalyzes the decarboxylation of 3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol
Cellular Location:Cell membrane; Peripheral membrane protein (Probable)
SMPDB Pathways:
  • Secondary Metabolites: Ubiquinol biosynthesis PW000981
  • Secondary Metabolites: Ubiquinol biosynthesis 2 PW002036
KEGG Pathways:
  • Metabolic pathways eco01100
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.03-Octaprenyl-4-hydroxybenzoate + 1.0Hydrogen ion → 1.02-Octaprenylphenol + 1.0Carbon dioxide
ReactionCard
1.02-Polyprenylphenol+1.0Thumb+1.0Thumb ↔ 1.04-Hydroxy-3-polyprenylbenzoate+1.0Thumb
1.02-Polyprenylphenol + 1.0Carbon dioxide + 1.04-Hydroxy-3-polyprenylbenzoate ↔ 1.04-Hydroxy-3-polyprenylbenzoate + 1.02-Polyprenylphenol
ReactionCard
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.03-Octaprenyl-4-hydroxybenzoate ↔ 1.02-Octaprenylphenol + 1.0Carbon dioxide
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.03-Octaprenyl-4-hydroxybenzoate + 1.0Hydrogen ion → 1.02-Octaprenylphenol + 1.0Carbon dioxide
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB041202-OctaprenylphenolMetaboCard
ECMDB238132-PolyprenylphenolMetaboCard
ECMDB211693-Octaprenyl-4-hydroxybenzoateMetaboCard
ECMDB237564-Hydroxy-3-polyprenylbenzoateMetaboCard
ECMDB04030Carbon dioxideMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
plasma membrane
Function
carbon-carbon lyase activity
carboxy-lyase activity
catalytic activity
lyase activity
Process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
oxidoreduction coenzyme metabolic process
ubiquinone biosynthetic process
ubiquinone metabolic process
Gene Properties
Blattner:b3843
Gene OrientationClockwise
Centisome Percentage:86.71
Left Sequence End4023011
Right Sequence End4024504
Gene Sequence:
>1494 bp
ATGGACGCCATGAAATATAACGATTTACGCGACTTCTTGACGCTGCTTGAACAGCAGGGT
GAGCTAAAACGTATCACGCTCCCGGTGGATCCGCATCTGGAAATCACTGAAATTGCTGAC
CGCACTTTGCGTGCCGGTGGGCCTGCGCTGTTGTTCGAAAACCCTAAAGGCTACTCAATG
CCGGTGCTGTGCAACCTGTTCGGTACGCCAAAGCGCGTGGCGATGGGCATGGGGCAGGAA
GATGTTTCGGCGCTGCGTGAAGTTGGTAAATTATTGGCGTTTCTGAAAGAGCCGGAGCCG
CCAAAAGGTTTCCGCGACCTGTTTGATAAACTGCCGCAGTTTAAGCAAGTATTGAACATG
CCGACAAAGCGGCTGCGTGGTGCGCCCTGCCAACAAAAAATCGTCTCTGGCGATGACGTC
GATCTCAATCGCATTCCCATTATGACCTGCTGGCCGGAAGATGCCGCGCCGCTGATTACC
TGGGGGCTGACAGTGACGCGCGGCCCACATAAAGAGCGGCAGAATCTGGGCATTTATCGC
CAGCAGCTGATTGGTAAAAACAAACTGATTATGCGCTGGCTGTCGCATCGCGGCGGCGCG
CTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCG
CTGGGTGCCGATCCCGCCACGATTCTCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCA
GAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCC
AATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGC
GAAACTGCGCCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGT
TTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACC
TATACCGGGCGTCCGCCAGATGAGCCCGCGGTGCTGGGTGTCGCACTGAACGAAGTGTTT
GTGCCGATTCTGCAAAAACAGTTCCCGGAAATTGTCGATTTTTACCTGCCGCCGGAAGGC
TGCTCTTATCGCCTGGCGGTAGTGACAATCAAAAAACAGTACGCCGGACACGCGAAGCGC
GTCATGATGGGCGTCTGGTCGTTCTTACGCCAGTTTATGTACACTAAATTTGTGATCGTT
TGCGATGATGACGTTAACGCACGCGACTGGAACGATGTGATTTGGGCGATTACCACCCGT
ATGGACCCGGCGCGGGATACTGTTCTGGTAGAAAATACGCCTATTGATTATCTGGATTTT
GCCTCGCCTGTCTCCGGGCTGGGTTCAAAAATGGGGCTGGATGCCACGAATAAATGGCCG
GGGGAAACCCAGCGTGAATGGGGACGTCCCATCAAAAAAGATCCAGATGTTGTCGCGCAT
ATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:497
Protein Molecular Weight:55603
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3-octaprenyl-4-hydroxybenzoate carboxy-lyase
MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLRAGGPALLFENPKGYSM
PVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPKGFRDLFDKLPQFKQVLNM
PTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLITWGLTVTRGPHKERQNLGIYR
QQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVSVALGADPATILGAVTPVPDTLS
EYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGYIEQGETAPEGPYGDHTGYYNEVDS
FPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGVALNEVFVPILQKQFPEIVDFYLPPEG
CSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQFMYTKFVIVCDDDVNARDWNDVIWAITTR
MDPARDTVLVENTPIDYLDFASPVSGLGSKMGLDATNKWPGETQREWGRPIKKDPDVVAH
IDAIWDELAIFNNGKSA
References
External Links:
ResourceLink
Uniprot ID:P0AAB4
Uniprot Name:UBID_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676210
Ecogene ID:EG11396
Ecocyc:EG11396
ColiBase:b3843
Kegg Gene:b3843
EchoBASE ID:EB1369
CCDB:UBID_ECOLI
BacMap:16131689
General Reference:
  • Bailey, M. J., Koronakis, V., Schmoll, T., Hughes, C. (1992). "Escherichia coli HlyT protein, a transcriptional activator of haemolysin synthesis and secretion, is encoded by the rfaH (sfrB) locus required for expression of sex factor and lipopolysaccharide genes." Mol Microbiol 6:1003-1012. Pubmed: 1584020
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Leppik, R. A., Young, I. G., Gibson, F. (1976). "Membrane-associated reactions in ubiquinone biosynthesis in Escherichia coli. 3-Octaprenyl-4-hydroxybenzoate carboxy-lyase." Biochim Biophys Acta 436:800-810. Pubmed: 782527
  • Zhang, H., Javor, G. T. (2000). "Identification of the ubiD gene on the Escherichia coli chromosome." J Bacteriol 182:6243-6246. Pubmed: 11029449