Probable galactarate transporter (P0AA80)

Identification
Name:Probable galactarate transporter
Synonyms:
  • D-galactarate permease
Gene Name:garP
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Uptake of D-galactarate
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00639Galactaric acidMetaboCard
ECMDB00663Glucaric acidMetaboCard
ECMDB04077Glyceric acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
transporter activity
Process
establishment of localization
transmembrane transport
transport
Gene Properties
Blattner:b3127
Gene OrientationCounterclockwise
Centisome Percentage:70.51
Left Sequence End3271595
Right Sequence End3272929
Gene Sequence:
>1335 bp
ATGACTGTAACGACTTTTTCCGAACTTGAACTCGACGAAAGCCTGCTGGAAGCCCTCCAG
GATAAAGGTTTCACTCGCCCGACCGCCATTCAGGCTGCCGCCATTCCGCCTGCGCTCGAT
GGCCGTGATGTACTCGGTTCTGCGCCGACAGGCACCGGTAAAACGGCGGCGTATCTGCTG
CCAGCGTTGCAGCACCTGCTCGATTTCCCGCGTAAGAAATCCGGTCCGCCGCGTATTTTG
ATCCTCACCCCAACTCGCGAGCTGGCGATGCAGGTGTCCGATCATGCCCGCGAACTGGCG
AAACATACGCATCTGGATATCGCCACCATCACCGGCGGCGTAGCCTATATGAACCACGCG
GAAGTGTTCAGCGAAAATCAGGACATCGTGGTCGCCACGACCGGACGTCTGCTGCAATAC
ATAAAAGAAGAGAACTTCGATTGCCGCGCGGTTGAAACGCTGATCCTCGACGAAGCAGAC
CGTATGCTGGATATGGGCTTCGCTCAGGATATCGAACATATTGCTGGCGAAACGCGCTGG
CGTAAACAGACCCTGCTCTTTTCGGCAACGCTGGAAGGCGATGCGATTCAGGACTTTGCC
GAGCGTCTGCTGGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAA
AAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTG
CATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGT
GTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGT
GAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAAC
GTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTC
TTTAACTTCGATATGCCGCGCAGTGGCGATACTTATTTGCACCGTATCGGACGTACCGCG
CGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTG
GGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGC
CCGAAAACGCGTGCGCCAAGCGAAAAGCAGACCGGCAAGCCATCGAAGAAAGTACTGGCT
AAACGTGCTGAGAAGAAAAAAGCTAAAGAGAAAGAGAAGCCGCGGGTGAAAAAACGCCAT
CGCGACACCAAAAATATTGGTAAGCGCCGTAAACCAAGCGGAACGGGCGTGCCACCGCAA
ACGACAGAAGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:444
Protein Molecular Weight:49009
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 12-32
  • 57-77
  • 90-110
  • 111-131
  • 158-178
  • 179-199
  • 253-273
  • 289-309
  • 320-340
  • 351-371
  • 386-406
  • 414-434
Protein Sequence:
>Probable galactarate transporter
MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFS
AFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRF
MLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHV
FTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKL
HYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGF
AGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFG
KGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVG
CSALMAMVCYLFVVGDIKRMELQK
References
External Links:
ResourceLink
Uniprot ID:P0AA80
Uniprot Name:GARP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675467
Ecogene ID:EG12760
Ecocyc:EG12760
ColiBase:b3127
Kegg Gene:b3127
CCDB:GARP_ECOLI
BacMap:16131019
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hubbard, B. K., Koch, M., Palmer, D. R., Babbitt, P. C., Gerlt, J. A. (1998). "Evolution of enzymatic activities in the enolase superfamily: characterization of the (D)-glucarate/galactarate catabolic pathway in Escherichia coli." Biochemistry 37:14369-14375. Pubmed: 9772162
  • Monterrubio, R., Baldoma, L., Obradors, N., Aguilar, J., Badia, J. (2000). "A common regulator for the operons encoding the enzymes involved in D-galactarate, D-glucarate, and D-glycerate utilization in Escherichia coli." J Bacteriol 182:2672-2674. Pubmed: 10762278