2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (P0A9D8)

Identification
Name:2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
Synonyms:
  • Tetrahydrodipicolinate N-succinyltransferase
  • THDP succinyltransferase
  • THP succinyltransferase
  • Tetrahydropicolinate succinylase
Gene Name:dapD
Enzyme Class:
Biological Properties
General Function:Involved in 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity
Specific Function:Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine- 2,6-dicarboxylate + H(2)O = CoA + N-succinyl-L-2-amino-6- oxoheptanedioate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Water + 1.0Succinyl-CoA + 1.0Tetrahydrodipicolinate ↔ 1.0Coenzyme A + 1.0N-Succinyl-2-amino-6-ketopimelate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Succinyl-CoA+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0(S)-2,3,4,5-tetrahydrodipicolinate + 1.0Succinyl-CoA + 1.0Water + 1.0Succinyl-CoA → 1.0Coenzyme A + 1.0N-Succinyl-2-amino-6-ketopimelate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Water + 1.0Succinyl-CoA + 1.0Tetrahydrodipicolinate ↔ 1.0Coenzyme A + 1.0N-Succinyl-2-amino-6-ketopimelate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24187(S)-2,3,4,5-tetrahydrodipicolinateMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB12266N-Succinyl-2-amino-6-ketopimelateMetaboCard
ECMDB01022Succinyl-CoAMetaboCard
ECMDB12289TetrahydrodipicolinateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity
acyltransferase activity
catalytic activity
N-acyltransferase activity
N-succinyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
aspartate family amino acid metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
lysine biosynthetic process
lysine biosynthetic process via diaminopimelate
lysine metabolic process
metabolic process
Gene Properties
Blattner:b0166
Gene OrientationCounterclockwise
Centisome Percentage:3.99
Left Sequence End185123
Right Sequence End185947
Gene Sequence:
>825 bp
ATGCAGCAGTTACAGAACATTATTGAAACCGCTTTTGAACGCCGTGCCGAGATCACGCCA
GCCAATGCAGACACCGTTACCCGCGAAGCGGTAAATCAGGTGATCGCCCTGCTGGATTCC
GGCGCACTGCGTGTAGCGGAAAAAATTGACGGTCAGTGGGTGACGCATCAGTGGTTGAAA
AAAGCGGTGCTGCTCTCTTTCCGTATTAATGATAATCAGGTGATCGAAGGGGCAGAAAGC
CGCTACTTCGACAAAGTGCCGATGAAATTCGCCGACTACGACGAAGCACGTTTCCAGAAA
GAAGGCTTCCGCGTTGTGCCACCAGCGGCGGTACGTCAGGGTGCGTTTATTGCCCGTAAC
ACCGTGCTGATGCCGTCTTACGTCAACATCGGCGCATATGTTGATGAAGGCACCATGGTT
GATACCTGGGCGACCGTCGGTTCTTGTGCGCAGATTGGTAAAAACGTCCACCTTTCCGGT
GGCGTGGGCATCGGCGGCGTGCTGGAACCGCTGCAGGCTAACCCAACCATCATTGAAGAT
AATTGCTTCATCGGCGCGCGCTCTGAAGTGGTTGAAGGGGTGATTGTCGAAGAAGGTTCC
GTCATTTCCATGGGCGTATACATTGGTCAGAGCACCCGTATTTACGACCGTGAAACCGGC
GAAATCCACTACGGTCGCGTTCCGGCGGGGTCTGTGGTTGTTTCAGGTAATCTGCCGTCA
AAAGATGGCAAATACAGCCTCTACTGTGCGGTTATCGTTAAGAAAGTTGACGCGAAAACT
CGCGGCAAAGTCGGCATTAACGAACTGCTGCGTACCATCGACTAA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:274
Protein Molecular Weight:29892
Protein Theoretical pI:5
PDB File:1KGT
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALRVAEKIDGQWVTHQWLK
KAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEGFRVVPPAAVRQGAFIARN
TVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPLQANPTIIED
NCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRETGEIHYGRVPAGSVVVSGNLPS
KDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID
References
External Links:
ResourceLink
Uniprot ID:P0A9D8
Uniprot Name:DAPD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21239021
PDB ID:1KGT
Ecogene ID:EG10207
Ecocyc:EG10207
ColiBase:b0166
Kegg Gene:b0166
EchoBASE ID:EB0203
CCDB:DAPD_ECOLI
BacMap:16128159
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Richaud, C., Richaud, F., Martin, C., Haziza, C., Patte, J. C. (1984). "Regulation of expression and nucleotide sequence of the Escherichia coli dapD gene." J Biol Chem 259:14824-14828. Pubmed: 6094577
  • van Heeswijk, W. C., Rabenberg, M., Westerhoff, H. V., Kahn, D. (1993). "The genes of the glutamine synthetase adenylylation cascade are not regulated by nitrogen in Escherichia coli." Mol Microbiol 9:443-457. Pubmed: 8412694