Fructose-1,6-bisphosphatase class 2 (P0A9C9)

Identification
Name:Fructose-1,6-bisphosphatase class 2
Synonyms:
  • FBPase class 2
  • D-fructose-1,6-bisphosphate 1-phosphohydrolase class 2
Gene Name:glpX
Enzyme Class:
Biological Properties
General Function:Involved in glycerol metabolic process
Specific Function:D-fructose 1,6-bisphosphate + H(2)O = D- fructose 6-phosphate + phosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Gluconeogenesis from L-malic acid PW000819
  • glycerol metabolism PW000914
  • glycerol metabolism II PW000915
  • glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
  • glycerol metabolism IV (glycerophosphoglycerol) PW000917
  • glycerol metabolism V (glycerophosphoserine) PW000918
  • glycolysis and pyruvate dehydrogenase PW000785
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Fructose 1,6-bisphosphate + 1.0Water ↔ 1.0Fructose 6-phosphate + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0beta-D-Fructose 1,6-bisphosphate + 1.0Water ↔ 1.0beta-D-Fructose 6-phosphate + 1.0Phosphate
ReactionCard
SMPDB Reactions:
1.0Fructose 6-phosphate+1.0Thumb+1.0Thumb → 1.0Fructose 1,6-bisphosphate+1.0Thumb+1.0Thumb
1.0Fructose 6-phosphate + 1.0Phosphate + 1.0Fructose 6-phosphate → 1.0Fructose 1,6-bisphosphate + 1.0Water + 1.0Fructose 1,6-bisphosphate
ReactionCard
1.0Fructose 1,6-bisphosphate+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Fructose 1,6-bisphosphate + 1.0Water + 1.0Fructose 1,6-bisphosphate → 1.0Phosphate + 1.0D-tagatofuranose 6-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Fructose 1,6-bisphosphate + 1.0Water ↔ 1.0Fructose 6-phosphate + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20123beta-D-Fructose 1,6-bisphosphateMetaboCard
ECMDB03971beta-D-Fructose 6-phosphateMetaboCard
ECMDB24205D-tagatofuranose 6-phosphateMetaboCard
ECMDB01058Fructose 1,6-bisphosphateMetaboCard
ECMDB00124Fructose 6-phosphateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Process
alcohol metabolic process
alditol metabolic process
glycerol metabolic process
metabolic process
polyol metabolic process
small molecule metabolic process
Gene Properties
Blattner:b3925
Gene OrientationCounterclockwise
Centisome Percentage:88.64
Left Sequence End4112592
Right Sequence End4113602
Gene Sequence:
>1011 bp
ATGTACTACCCCTTCGTTCGTAAAGCCCTTTTCCAGCTCGATCCAGAGCGCGCTCATGAG
TTTACTTTTCAGCAATTACGCCGTATTACAGGAACGCCGTTTGAAGCACTGGTGCGGCAG
AAAGTGCCTGCGAAACCTGTTAACTGCATGGGCCTGACGTTTAAAAATCCGCTTGGTCTG
GCAGCCGGTCTTGATAAAGACGGGGAGTGCATTGACGCGTTAGGCGCGATGGGATTTGGA
TCGATCGAGATCGGTACCGTCACGCCACGTCCACAGCCAGGTAATGACAAGCCGCGTCTC
TTTCGTCTGGTAGATGCCGAAGGTTTGATCAACCGTATGGGCTTTAATAATCTTGGCGTT
GATAACCTCGTAGAGAACGTAAAAAAGGCCCATTATGACGGCGTCCTGGGTATTAACATC
GGCAAAAATAAAGATACGCCAGTGGAGCAGGGCAAAGATGACTATCTGATTTGTATGGAA
AAAATCTATGCCTATGCGGGATATATCGCCATCAATATTTCATCGCCGAATACCCCAGGA
TTACGCACGCTGCAATATGGTGAAGCGCTGGATGATCTCTTAACCGCGATTAAAAATAAG
CAAAATGATTTGCAAGCGATGCACCATAAATATGTGCCGATCGCAGTGAAGATCGCGCCG
GATCTTTCTGAAGAAGAATTGATCCAGGTTGCCGATAGTTTAGTTCGCCATAATATTGAT
GGCGTTATTGCAACCAATACCACACTCGATCGTTCTCTTGTTCAGGGAATGAAAAATTGC
GATCAAACCGGTGGCTTAAGTGGTCGTCCGCTTCAGTTAAAAAGCACCGAAATTATTCGC
CGCTTGTCACTGGAATTAAACGGTCGCTTACCGATCATCGGTGTTGGCGGCATCGACTCG
GTTATCGCTGCGCGTGAAAAGATTGCTGCGGGTGCCTCACTGGTGCAAATTTATTCTGGT
TTTATTTTTAAAGGTCCGCCGCTGATTAAAGAAATCGTTACCCATATCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:336
Protein Molecular Weight:35852
Protein Theoretical pI:5
PDB File:1NI9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Fructose-1,6-bisphosphatase class 2
MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIMLNQVNIDGTIVIGEGEI
DEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALAVLAVGDKGCFLNAPDMYM
EKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVTILAKPRHDAVIAEMQQLGVR
VFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSAAVIRALDGDMNGRLLARHDVKG
DNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSDNVIFSATGITKGDLLEGISRKGNI
ATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVHIL
References
External Links:
ResourceLink
Uniprot ID:P0A9C9
Uniprot Name:GLPX_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651460
PDB ID:1NI9
Ecogene ID:EG11517
Ecocyc:EG11517
ColiBase:b3925
Kegg Gene:b3925
EchoBASE ID:EB1479
CCDB:GLPX_ECOLI
BacMap:16131763
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Donahue, J. L., Bownas, J. L., Niehaus, W. G., Larson, T. J. (2000). "Purification and characterization of glpX-encoded fructose 1, 6-bisphosphatase, a new enzyme of the glycerol 3-phosphate regulon of Escherichia coli." J Bacteriol 182:5624-5627. Pubmed: 10986273
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Morimyo, M. (1988). "Isolation and characterization of methyl viologen-sensitive mutants of Escherichia coli K-12." J Bacteriol 170:2136-2142. Pubmed: 2834327
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
  • Truniger, V., Boos, W., Sweet, G. (1992). "Molecular analysis of the glpFKX regions of Escherichia coli and Shigella flexneri." J Bacteriol 174:6981-6991. Pubmed: 1400248