Orotate phosphoribosyltransferase (P0A7E3)

Identification
Name:Orotate phosphoribosyltransferase
Synonyms:
  • OPRT
  • OPRTase
Gene Name:pyrE
Enzyme Class:
Biological Properties
General Function:Involved in orotate phosphoribosyltransferase activity
Specific Function:Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Orotidylic acid + 1.0Pyrophosphate ↔ 1.0Orotic acid + 1.0Phosphoribosyl pyrophosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.0Thumb
1.0Orotic acid + 1.0Phosphoribosyl pyrophosphate → 1.0Pyrophosphate + 1.0Orotidylic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Orotidylic acid + 1.0Pyrophosphate ↔ 1.0Orotic acid + 1.0Phosphoribosyl pyrophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Orotidylic acid + 1.0Pyrophosphate → 1.0Orotic acid + 1.0Phosphoribosyl pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00226Orotic acidMetaboCard
ECMDB00218Orotidylic acidMetaboCard
ECMDB00280Phosphoribosyl pyrophosphateMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
catalytic activity
orotate phosphoribosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
Process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
pyrimidine nucleotide biosynthetic process
pyrimidine nucleotide metabolic process
Gene Properties
Blattner:b3642
Gene OrientationCounterclockwise
Centisome Percentage:82.19
Left Sequence End3813150
Right Sequence End3813791
Gene Sequence:
>642 bp
ATGCGCAGTAAGTATATCGTCATTGAGGGGCTGGAAGGCGCAGGCAAAACTACCGCGCGT
AATGTGGTGGTTGAGACGCTCGAGCAACTGGGTATCCGCGACATGGTTTTCACTCGGGAA
CCTGGCGGTACGCAACTTGCCGAAAAGTTAAGAAGCCTGGTGCTGGATATCAAATCGGTA
GGCGATGAAGTCATTACCGATAAAGCCGAAGTTCTGATGTTTTATGCCGCGCGCGTTCAA
CTGGTAGAAACGGTCATCAAACCAGCTCTGGCTAACGGCACCTGGGTGATTGGCGATCGC
CACGATCTCTCCACTCAGGCGTATCAGGGCGGCGGACGTGGTATTGACCAACATATGCTG
GCAACACTGCGTGATGCTGTTCTCGGGGATTTTCGCCCCGACTTAACGCTCTATCTCGAT
GTTACCCCGGAAGTTGGCTTAAAACGCGCGCGTGCGCGCGGCGAGCTGGATCGTATTGAG
CAAGAATCTTTCGATTTCTTTAATCGCACCCGCGCCCGCTATCTGGAACTGGCAGCACAA
GATAAAAGCATTCATACCATTGATGCCACCCAGCCGCTGGAGGCCGTGATGGATGCAATC
CGCACTACCGTGACCCACTGGGTGAAGGAGTTGGACGCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:213
Protein Molecular Weight:23567
Protein Theoretical pI:5
PDB File:1ORO
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Orotate phosphoribosyltransferase
MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGRDLALLGRFYAEALVDS
GIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEAKDHGEGGNLVGSALQGRV
MLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERGRGEISAIQEVERDYNCKVIS
IITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV
References
External Links:
ResourceLink
Uniprot ID:P0A7E3
Uniprot Name:PYRE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062665
PDB ID:1ORO
Ecogene ID:EG10808
Ecocyc:EG10808
ColiBase:b3642
Kegg Gene:b3642
EchoBASE ID:EB0801
CCDB:PYRE_ECOLI
BacMap:16131513
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Henriksen, A., Aghajari, N., Jensen, K. F., Gajhede, M. (1996). "A flexible loop at the dimer interface is a part of the active site of the adjacent monomer of Escherichia coli orotate phosphoribosyltransferase." Biochemistry 35:3803-3809. Pubmed: 8620002
  • Poulsen, P., Bonekamp, F., Jensen, K. F. (1984). "Structure of the Escherichia coli pyrE operon and control of pyrE expression by a UTP modulated intercistronic attentuation." EMBO J 3:1783-1790. Pubmed: 6207018
  • Poulsen, P., Jensen, K. F., Valentin-Hansen, P., Carlsson, P., Lundberg, L. G. (1983). "Nucleotide sequence of the Escherichia coli pyrE gene and of the DNA in front of the protein-coding region." Eur J Biochem 135:223-229. Pubmed: 6349999