UDP-N-acetylglucosamine 1-carboxyvinyltransferase (P0A749)

Identification
Name:UDP-N-acetylglucosamine 1-carboxyvinyltransferase
Synonyms:
  • Enoylpyruvate transferase
  • UDP-N-acetylglucosamine enolpyruvyl transferase
  • EPT
Gene Name:murA
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring alkyl or aryl (other than methyl) groups
Specific Function:Cell wall formation. Adds enolpyruvyl to UDP-N- acetylglucosamine. Target for the antibiotic phosphomycin
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Uridine diphosphate-N-acetylglucosamine ↔ 1.0Phosphate + 1.0UDP-N-Acetyl-3-(1-carboxyvinyl)-D-glucosamine
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine + 1.0Phosphoenolpyruvic acid → 1.0Phosphate + 1.0UDP-N-acetyl-α-D-glucosamine-enolpyruvate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Uridine diphosphate-N-acetylglucosamine ↔ 1.0Phosphate + 1.0UDP-N-Acetyl-3-(1-carboxyvinyl)-D-glucosamine
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine + 1.0Phosphoenolpyruvic acid → 1.0UDP-N-Acetyl-3-(1-carboxyvinyl)-D-glucosamine + 1.0Phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Phosphoenolpyruvic acid + 1.0Uridine diphosphate-N-acetylglucosamine → 1.0Inorganic phosphate + 1.0UDP-N-Acetyl-3-(1-carboxyvinyl)-D-glucosamine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB04167UDP-N-Acetyl-3-(1-carboxyvinyl)-D-glucosamineMetaboCard
ECMDB24237UDP-N-acetyl-α-D-glucosamine-enolpyruvateMetaboCard
ECMDB00290Uridine diphosphate-N-acetylglucosamineMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Process
alcohol metabolic process
amino sugar metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
UDP-N-acetylgalactosamine biosynthetic process
UDP-N-acetylgalactosamine metabolic process
Gene Properties
Blattner:b3189
Gene OrientationCounterclockwise
Centisome Percentage:71.84
Left Sequence End3333257
Right Sequence End3334516
Gene Sequence:
>1260 bp
ATGGATAAATTTCGTGTTCAGGGGCCAACGAAGCTCCAGGGCGAAGTCACAATTTCCGGC
GCTAAAAATGCTGCTCTGCCTATCCTTTTTGCCGCTCTACTGGCGGAAGAACCGGTAGAG
ATCCAGAACGTCCCGAAACTGAAAGACGTCGATACATCAATGAAGCTGCTAAGCCAGCTG
GGTGCGAAAGTAGAACGTAATGGTTCTGTGCATATTGATGCCCGCGACGTTAATGTATTC
TGCGCACCTTACGATCTGGTTAAAACCATGCGTGCTTCTATCTGGGCACTGGGGCCGCTG
GTAGCGCGCTTTGGTCAGGGGCAAGTTTCACTGCCTGGCGGTTGTACGATCGGTGCGCGT
CCGGTTGATCTACACATTTCTGGCCTCGAACAATTAGGCGCGACCATCAAACTGGAAGAA
GGTTACGTTAAAGCTTCCGTCGATGGTCGTCTGAAAGGCGCACATATCGTGATGGATAAA
GTCAGCGTTGGCGCAACGGTGACCATCATGTGTGCTGCAACCCTGGCGGAAGGTACCACG
ATTATTGAAAACGCAGCGCGTGAACCGGAAATCGTCGATACCGCGAACTTCCTGATTACG
CTGGGTGCGAAAATTAGCGGTCAGGGCACCGATCGTATCGTCATCGAAGGTGTGGAACGT
TTAGGCGGCGGTGTCTATCGCGTGCTACCGGATCGTATCGAAACCGGTACTTTCCTGGTG
GCGGCGGCGATTTCTCGCGGCAAAATTATCTGCCGTAACGCGCAGCCAGATACTCTCGAC
GCCGTGCTGGCGAAACTGCGTGACGCTGGAGCGGACATCGAAGTCGGCGAAGACTGGATT
AGCCTGGATATGCATGGCAAACGTCCGAAGGCTGTTAACGTACGTACCGCGCCGCATCCG
GCATTCCCGACCGATATGCAGGCCCAGTTCACGCTGTTGAACCTGGTGGCAGAAGGGACC
GGATTCATCACCGAAACGGTCTTTGAAAACCGCTTTATGCATGTGCCGGAGCTTAGCCGT
ATGGGCGCGCATGCCGAAATCGAAAGCAATACCGTTATTTGTCACGGTGTTGAAAAACTT
TCTGGCGCACAGGTTATGGCAACCGATCTGCGTGCATCAGCAAGCCTGGTGCTGGCTGGC
TGTATTGCGGAAGGGACGACGGTGGTTGATCGTATTTATCACATCGATCGTGGCTACGAA
CGCATTGAAGACAAACTGCGCGCTTTAGGTGCAAATATTGAGCGTGTGAAAGGCGAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:419
Protein Molecular Weight:44817
Protein Theoretical pI:6
PDB File:1UAE
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>UDP-N-acetylglucosamine 1-carboxyvinyltransferase
MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNVPKLKDVDTSMKLLSQL
GAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVARFGQGQVSLPGGCTIGAR
PVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDKVSVGATVTIMCAATLAEGTT
IIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGVERLGGGVYRVLPDRIETGTFLV
AAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVGEDWISLDMHGKRPKAVNVRTAPHP
AFPTDMQAQFTLLNLVAEGTGFITETVFENRFMHVPELSRMGAHAEIESNTVICHGVEKL
SGAQVMATDLRASASLVLAGCIAEGTTVVDRIYHIDRGYERIEDKLRALGANIERVKGE
References
External Links:
ResourceLink
Uniprot ID:P0A749
Uniprot Name:MURA_ECOLI
GenBank Gene ID:AB028039
Genebank Protein ID:4972322
PDB ID:1UAE
Ecogene ID:EG11358
Ecocyc:EG11358
ColiBase:b3189
Kegg Gene:b3189
EchoBASE ID:EB1333
CCDB:MURA_ECOLI
BacMap:16131079
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Horii, T., Kimura, T., Sato, K., Shibayama, K., Ohta, M. (1999). "Emergence of fosfomycin-resistant isolates of Shiga-like toxin-producing Escherichia coli O26." Antimicrob Agents Chemother 43:789-793. Pubmed: 10103182
  • Marquardt, J. L., Siegele, D. A., Kolter, R., Walsh, C. T. (1992). "Cloning and sequencing of Escherichia coli murZ and purification of its product, a UDP-N-acetylglucosamine enolpyruvyl transferase." J Bacteriol 174:5748-5752. Pubmed: 1512209
  • Skarzynski, T., Kim, D. H., Lees, W. J., Walsh, C. T., Duncan, K. (1998). "Stereochemical course of enzymatic enolpyruvyl transfer and catalytic conformation of the active site revealed by the crystal structure of the fluorinated analogue of the reaction tetrahedral intermediate bound to the active site of the C115A mutant of MurA." Biochemistry 37:2572-2577. Pubmed: 9485407
  • Skarzynski, T., Mistry, A., Wonacott, A., Hutchinson, S. E., Kelly, V. A., Duncan, K. (1996). "Structure of UDP-N-acetylglucosamine enolpyruvyl transferase, an enzyme essential for the synthesis of bacterial peptidoglycan, complexed with substrate UDP-N-acetylglucosamine and the drug fosfomycin." Structure 4:1465-1474. Pubmed: 8994972