Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (P0A722)

Identification
Name:Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
Synonyms:
  • UDP-N-acetylglucosamine acyltransferase
Gene Name:lpxA
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity
Specific Function:Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein]+1.0Thumb+1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] ↔ 1.0Acyl-carrier protein+1.0Thumb+1.0Acyl-carrier protein
1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] + 1.0Uridine diphosphate-N-acetylglucosamine + 1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] ↔ 1.0Acyl-carrier protein + 1.0UDP-3-O-(3-Hydroxymyristoyl)-N-acetylglucosamine + 1.0Acyl-carrier protein
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0(3R)-3-hydroxymyristoyl-[acp] → 1.0a holo-[acyl-carrier protein]+1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine + 1.0(3R)-3-hydroxymyristoyl-[acp] → 1.0a holo-[acyl-carrier protein] + 1.0UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-α-D-glucosamine
ReactionCard
Complex Reactions:
1.0(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein]+1.0Thumb ↔ 1.0acyl carrier protein+1.0Thumb
1.0(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] + 1.0Uridine diphosphate-N-acetylglucosamine ↔ 1.0acyl carrier protein + 1.0UDP-3-O-(3-Hydroxymyristoyl)-N-acetylglucosamine
ReactionCard
1.0(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein]+1.0Thumb → 1.0[acyl-carrier-protein]+1.0Thumb
1.0(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + 1.0Uridine diphosphate-N-acetylglucosamine → 1.0[acyl-carrier-protein] + 1.0UDP-3-O-(3-Hydroxymyristoyl)-N-acetylglucosamine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04164UDP-3-O-(3-Hydroxymyristoyl)-N-acetylglucosamineMetaboCard
ECMDB24215UDP-3-O-[(3R)-3-hydroxymyristoyl]-N-acetyl-α-D-glucosamineMetaboCard
ECMDB00290Uridine diphosphate-N-acetylglucosamineMetaboCard
GO Classification:
Function
acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity
acyltransferase activity
catalytic activity
O-acyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
lipid biosynthetic process
lipid metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b0181
Gene OrientationClockwise
Centisome Percentage:4.37
Left Sequence End202560
Right Sequence End203348
Gene Sequence:
>789 bp
GTGATTGATAAATCCGCCTTTGTGCATCCAACCGCCATTGTGGAAGAGGGCGCGTCAATT
GGCGCGAACGCACACATTGGTCCTTTTTGTATCGTTGGACCCCATGTCGAAATTGGTGAG
GGTACCGTACTGAAATCTCACGTTGTCGTGAATGGTCATACTAAAATTGGCCGCGATAAT
GAGATTTATCAGTTCGCCTCCATCGGCGAAGTTAACCAGGATCTGAAATATGCTGGCGAA
CCGACCCGTGTGGAAATCGGCGATCGTAACCGCATTCGCGAAAGCGTCACCATTCATCGT
GGCACAGTCCAGGGCGGTGGATTGACGAAGGTGGGCAGCGACAACTTACTGATGATCAAC
GCGCACATTGCGCACGATTGTACGGTAGGTAACCGCTGTATTCTCGCCAACAACGCAACG
CTGGCGGGTCACGTATCGGTTGACGACTTCGCGATCATCGGCGGCATGACCGCAGTCCAT
CAGTTCTGCATCATTGGTGCGCACGTGATGGTTGGCGGCTGCTCCGGTGTGGCGCAGGAC
GTCCCTCCTTATGTCATTGCGCAGGGTAACCACGCAACGCCGTTCGGTGTCAATATCGAA
GGGCTGAAGCGCCGCGGATTCAGCCGTGAGGCGATTACCGCTATCCGCAATGCGTATAAG
CTGATTTATCGTAGCGGTAAAACGCTCGATGAAGTGAAACCGGAAATTGCTGAACTGGCG
GAAACATATCCGGAAGTGAAAGCCTTTACCGATTTCTTTGCACGCTCAACGCGCGGTCTG
ATTCGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:262
Protein Molecular Weight:28080
Protein Theoretical pI:7
PDB File:1LXA
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVLKSHVVVNGHTKIGRDN
EIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGTVQGGGLTKVGSDNLLMIN
AHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVHQFCIIGAHVMVGGCSGVAQD
VPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNAYKLIYRSGKTLDEVKPEIAELA
ETYPEVKAFTDFFARSTRGLIR
References
External Links:
ResourceLink
Uniprot ID:P0A722
Uniprot Name:LPXA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674370
PDB ID:1LXA
Ecogene ID:EG10545
Ecocyc:EG10545
ColiBase:b0181
Kegg Gene:b0181
EchoBASE ID:EB0540
CCDB:LPXA_ECOLI
BacMap:16128174
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Coleman, J., Raetz, C. R. (1988). "First committed step of lipid A biosynthesis in Escherichia coli: sequence of the lpxA gene." J Bacteriol 170:1268-1274. Pubmed: 3277952
  • Crowell, D. N., Reznikoff, W. S., Raetz, C. R. (1987). "Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase." J Bacteriol 169:5727-5734. Pubmed: 2824445
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Raetz, C. R., Roderick, S. L. (1995). "A left-handed parallel beta helix in the structure of UDP-N-acetylglucosamine acyltransferase." Science 270:997-1000. Pubmed: 7481807