ECMDB

Identification

Name:

2-keto-3-deoxygluconate permease

Synonyms:

KDG permease

Gene Name:

kdgT

Enzyme Class:

Not Available

Biological Properties

General Function:

Involved in 2-keto-3-deoxygluconate:hydrogen symporter activity

Specific Function:

The 2-keto-3-deoxygluconate permease transports the degraded pectin products into the bacterial cell, where they serve as carbon and energy sources. This is a hydrogen coupled transport system

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

hexuronide and hexuronate degradation PW000834
inner membrane transport PW000786

KEGG Pathways:

Not Available

Transports:

Transport References:

Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590

Metabolites:

ECMDB ID	Name	View
ECMDB01353	2-Keto-3-deoxy-D-gluconic acid	MetaboCard
ECMDB04073	D-Glucuronic acid	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard

GO Classification:

Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Function
2-keto-3-deoxygluconate:hydrogen symporter activity
cation transmembrane transporter activity
cation:sugar symporter activity
ion transmembrane transporter activity
solute:cation symporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
carbohydrate transport
establishment of localization
transport

Gene Properties

Blattner:

b3909

Gene Orientation

Clockwise

Centisome Percentage:

88.36

Left Sequence End

4099713

Right Sequence End

4100696

Gene Sequence:

>984 bp
GTGGAAGCGATTAAGGGATCGGACGTTAATGTCCCGGATGCAGTATTTGCCTGGATGCTG
GATGGTAGAGGCGGCGTTAAACCGCTGGAAAATACAGATGTGATTGATGAAGCGCATCCC
TGTTGGCTCCACCTTAATTATGTACACCATGATAGCGCCCAATGGCTGGCGACAACACCG
CTGCTTCCCAATAACGTACGTGATGCGCTGGCGGGCGAGAGCACGCGTCCCCGAGTCAGC
CGTCTCGGTGAAGGCACGCTGATTACATTGCGCTGTATAAACGGCAGCACCGATGAACGC
CCCGATCAACTGGTCGCCATGCGTGTATATATGGACGGGCGGTTAATTGTTTCGACCCGA
CAACGCAAAGTGCTGGCGCTGGACGATGTGGTGAGCGATCTGGAAGAGGGCACGGGTCCG
ACCGATTGCGGGGGATGGCTGGTGGATGTGTGCGATGCGTTGACCGATCATTCCAGTGAA
TTTATCGAGCAGCTGCACGATAAAATTATCGACCTTGAAGATAATCTCCTTGATCAGCAA
ATTCCACCGCGTGGATTCCTGGCTCTGCTGCGCAAACAATTAATTGTGATGCGTCGCTAT
ATGGCACCGCAACGTGATGTTTATGCTCGTCTTGCCAGTGAACGTTTGCCGTGGATGAGC
GATGACCAACGCCGTCGGATGCAGGATATTGCCGATCGCCTTGGGCGCGGCCTTGACGAA
ATCGACGCCTGTATAGCACGGACTGGCGTGATGGCGGATGAAATCGCTCAGGTGATGCAG
GAAAATTTAGCTCGTCGTACCTATACAATGTCGTTGATGGCAATGGTCTTTTTACCCAGT
ACCTTTCTGACCGGGTTATTTGGCGTCAACCTTGGTGGGATCCCTGGCGGCGGGTGGCAA
TTCGGATTTTCAATTTTTTGTATTCTGTTAGTTGTTCTTATTGGTGGTGTTGCTTTATGG
TTGCATCGTAGTAAATGGTTGTAA

Protein Properties

Pfam Domain Function:

KdgT (PF03812 )

Protein Residues:

327

Protein Molecular Weight:

33669

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

10-30
42-62
73-93
95-115
139-159
163-183
199-219
224-244
254-274
289-309

Protein Sequence:

>2-keto-3-deoxygluconate permease
MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVPILAVWFFCMGA
SIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAGLSTLALVAAMDM
TNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASFEPHVFVGAVLPFLV
GFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGLLGILLGVAVIIVTGIP
LIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEMVPAFKPMAPAATSLVATAVIVTSI
LVPILTSIWSRKVKARAAKIEILGTVK

References

External Links:

Resource	Link
Uniprot ID:	P0A712
Uniprot Name:	KDGT_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674915
Ecogene ID:	EG11869
Ecocyc:	EG11869
ColiBase:	b3909
Kegg Gene:	b3909
EchoBASE ID:	EB1815
CCDB:	KDGT_ECOLI
BacMap:	90111665

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018

2-keto-3-deoxygluconate permease (P0A712)