Identification
Name:Glucose-6-phosphate isomerase
Synonyms:
  • GPI
  • Phosphoglucose isomerase
  • PGI
  • Phosphohexose isomerase
  • PHI
Gene Name:pgi
Enzyme Class:
Biological Properties
General Function:Involved in glucose-6-phosphate isomerase activity
Specific Function:D-glucose 6-phosphate = D-fructose 6- phosphate
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism III PW000895
  • Gluconeogenesis from L-malic acid PW000819
  • O-antigen building blocks biosynthesis PW002089
  • Starch and sucrose metabolism PW000941
  • colanic acid building blocks biosynthesis PW000951
  • glycerol metabolism PW000914
  • glycerol metabolism II PW000915
  • glycerol metabolism III (sn-glycero-3-phosphoethanolamine) PW000916
  • glycerol metabolism IV (glycerophosphoglycerol) PW000917
  • glycerol metabolism V (glycerophosphoserine) PW000918
  • glycolysis and pyruvate dehydrogenase PW000785
  • peptidoglycan biosynthesis I PW000906
  • peptidoglycan biosynthesis I 2 PW002062
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb
1.0Thumb1.0Thumb
1.0Thumb1.0Thumb
SMPDB Reactions:
1.0Thumb1.0Fructose 6-phosphate+1.0Thumb
1.0Thumb1.0β-D-glucose 6-phosphate
1.0D-tagatofuranose 6-phosphate → 1.0β-D-glucose 6-phosphate
ReactionCard
1.0 β-D-glucose 6-phosphate+1.0Thumb1.0Thumb
1.0Thumb1.0Thumb
1.0Thumb1.0Thumb
1.0β-D-glucose 6-phosphate1.0Thumb
1.0β-D-glucose 6-phosphate ↔ 1.0D-tagatofuranose 6-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb1.0Thumb
Complex Reactions:
1.0Thumb1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB24206 β-D-glucose 6-phosphateMetaboCard
ECMDB23064Alpha-D-glucose 6-phosphateMetaboCard
ECMDB03971beta-D-Fructose 6-phosphateMetaboCard
ECMDB03498beta-D-Glucose 6-phosphateMetaboCard
ECMDB24205D-tagatofuranose 6-phosphateMetaboCard
ECMDB00124Fructose 6-phosphateMetaboCard
ECMDB01401Glucose 6-phosphateMetaboCard
GO Classification:
Function
catalytic activity
glucose-6-phosphate isomerase activity
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, interconverting aldoses and ketoses
isomerase activity
Process
alcohol metabolic process
gluconeogenesis
glucose catabolic process
glucose metabolic process
glycolysis
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Blattner:b4025
Gene OrientationClockwise
Centisome Percentage:91.21
Left Sequence End4231781
Right Sequence End4233430
Gene Sequence:
>1650 bp
ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACAGAAACACTTCGAT
GAAATGAAAGACGTTACGATCGCCGATCTTTTTGCTAAAGACGGCGATCGTTTTTCTAAG
TTCTCCGCAACCTTCGACGATCAGATGCTGGTGGATTACTCCAAAAACCGCATCACTGAA
GAGACGCTGGCGAAATTACAGGATCTGGCGAAAGAGTGCGATCTGGCGGGCGCGATTAAG
TCGATGTTCTCTGGCGAGAAGATCAACCGCACTGAAAACCGCGCCGTGCTGCACGTAGCG
CTGCGTAACCGTAGCAATACCCCGATTTTGGTTGATGGCAAAGACGTAATGCCGGAAGTC
AACGCGGTGCTGGAGAAGATGAAAACCTTCTCAGAAGCGATTATTTCCGGTGAGTGGAAA
GGTTATACCGGCAAAGCAATCACTGACGTAGTGAACATCGGGATCGGCGGTTCTGACCTC
GGCCCATACATGGTGACCGAAGCTCTGCGTCCGTACAAAAACCACCTGAACATGCACTTT
GTTTCTAACGTCGATGGGACTCACATCGCGGAAGTGCTGAAAAAAGTAAACCCGGAAACC
ACGCTGTTCTTGGTAGCATCTAAAACCTTCACCACTCAGGAAACTATGACCAACGCCCAT
AGCGCGCGTGACTGGTTCCTGAAAGCGGCAGGTGATGAAAAACACGTTGCAAAACACTTT
GCGGCGCTTTCCACCAATGCCAAAGCCGTTGGCGAGTTTGGTATTGATACTGCCAACATG
TTCGAGTTCTGGGACTGGGTTGGCGGCCGTTACTCTTTGTGGTCAGCGATTGGCCTGTCG
ATTGTTCTCTCCATCGGCTTTGATAACTTCGTTGAACTGCTTTCCGGCGCACACGCGATG
GACAAGCATTTCTCCACCACGCCTGCCGAGAAAAACCTGCCTGTACTGCTGGCGCTGATT
GGCATCTGGTACAACAATTTCTTTGGTGCGGAAACTGAAGCGATTCTGCCGTATGACCAG
TATATGCACCGTTTCGCGGCGTACTTCCAGCAGGGCAATATGGAGTCCAACGGTAAGTAT
GTTGACCGTAACGGTAACGTTGTGGATTACCAGACTGGCCCGATTATCTGGGGTGAACCA
GGCACTAACGGTCAGCACGCGTTCTACCAGCTGATCCACCAGGGAACCAAAATGGTACCG
TGCGATTTCATCGCTCCGGCTATCACCCATAACCCGCTCTCTGATCATCACCAGAAACTG
CTGTCTAACTTCTTCGCCCAGACCGAAGCGCTGGCGTTTGGTAAATCCCGCGAAGTGGTT
GAGCAGGAATATCGTGATCAGGGTAAAGATCCGGCAACGCTTGACTACGTGGTGCCGTTC
AAAGTATTCGAAGGTAACCGCCCGACCAACTCCATCCTGCTGCGTGAAATCACTCCGTTC
AGCCTGGGTGCGTTGATTGCGCTGTATGAGCACAAAATCTTTACTCAGGGCGTGATCCTG
AACATCTTCACCTTCGACCAGTGGGGCGTGGAACTGGGTAAACAGCTGGCGAACCGTATT
CTGCCAGAGCTGAAAGATGATAAAGAAATCAGCAGCCACGATAGCTCGACCAATGGTCTG
ATTAACCGCTATAAAGCGTGGCGCGGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:549
Protein Molecular Weight:61529
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glucose-6-phosphate isomerase
MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSATFDDQMLVDYSKNRITE
ETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALRNRSNTPILVDGKDVMPEV
NAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDLGPYMVTEALRPYKNHLNMHF
VSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTNAHSARDWFLKAAGDEKHVAKHF
AALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSAIGLSIVLSIGFDNFVELLSGAHAM
DKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEAILPYDQYMHRFAAYFQQGNMESNGKY
VDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLIHQGTKMVPCDFIAPAITHNPLSDHHQKL
LSNFFAQTEALAFGKSREVVEQEYRDQGKDPATLDYVVPFKVFEGNRPTNSILLREITPF
SLGALIALYEHKIFTQGVILNIFTFDQWGVELGKQLANRILPELKDDKEISSHDSSTNGL
INRYKAWRG
References
External Links:
ResourceLink
Uniprot ID:P0A6T1
Uniprot Name:G6PI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676777
Ecogene ID:EG10702
Ecocyc:EG10702
ColiBase:b4025
Kegg Gene:b4025
EchoBASE ID:EB0696
CCDB:G6PI_ECOLI
BacMap:16131851
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Froman, B. E., Tait, R. C., Gottlieb, L. D. (1989). "Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coli." Mol Gen Genet 217:126-131. Pubmed: 2549364
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Smith, M. W., Doolittle, R. F. (1992). "Anomalous phylogeny involving the enzyme glucose-6-phosphate isomerase." J Mol Evol 34:544-545. Pubmed: 1593646
  • Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842