Identification
Name:Dihydrodipicolinate synthase
Synonyms:
  • DHDPS
Gene Name:dapA
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H(2)O
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Lysine biosynthesis PW000771
  • Secondary Metabolites: threonine biosynthesis from aspartate PW000976
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+2.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+2.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate+1.0Thumb
1.0L-Aspartate-semialdehyde + 1.0Pyruvic acid → 1.0Hydrogen ion + 1.0Water + 1.0(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + 1.0(2S,4S)-4-Hydroxy-2,3,4,5-tetrahydrodipicolinate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+2.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB23778(2S,4S)-4-Hydroxy-2,3,4,5-tetrahydrodipicolinateMetaboCard
ECMDB040562,3-Dihydrodipicolinic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB12249L-Aspartate-semialdehydeMetaboCard
ECMDB00243Pyruvic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
carbon-oxygen lyase activity
catalytic activity
dihydrodipicolinate synthase activity
hydro-lyase activity
lyase activity
Process
carboxylic acid biosynthetic process
cellular metabolic process
diaminopimelate biosynthetic process
metabolic process
organic acid biosynthetic process
organic acid metabolic process
Gene Properties
Blattner:b2478
Gene OrientationCounterclockwise
Centisome Percentage:55.97
Left Sequence End2596904
Right Sequence End2597782
Gene Sequence:
>879 bp
ATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTG
GCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGCTG
GGAATGAAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAG
GGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCAT
CACTACGGTCGTCATCCGGAGGCTCGCGAATGGTATCACCAGTGGGTTTACTTTCGTCCG
CGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTT
CGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCC
GGGCAAGGGGAAGGGCGCTATTCGGAGCTGCTGGCGATAAATCTGCTTGAGCAATTGTTA
CTGCGGCGCATGGAAGCGATTAACGAGTCGCTCCATCCACCGATGGATAATCGGGTACGC
GAGGCTTGTCAGTACATCAGCGATCACCTGGCAGACAGCAATTTTGATATCGCCAGCGTC
GCACAGCATGTTTGCTTGTCGCCGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGG
ATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGC
ACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAT
TTCTCGCGAGTATTTAAAAAATGCACCGGGGCCAGCCCGAGCGAGTTTCGTGCCGGTTGT
GAAGAAAAAGTGAATGATGTAGCCGTCAAGTTGTCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:292
Protein Molecular Weight:31270
Protein Theoretical pI:6
PDB File:1DHP
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Dihydrodipicolinate synthase
MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGTTGESATLNHDEHADVV
MMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLTVTPYYNRPSQEGLYQHFK
AIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGIKEATGNLTRVNQIKELVSDD
FVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMCKLAAEGHFAEARVINQRLMPLH
NKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITDSGRETVRAALKHAGLL
References
External Links:
ResourceLink
Uniprot ID:P0A6L2
Uniprot Name:DAPA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321946
PDB ID:1DHP
Ecogene ID:EG10205
Ecocyc:EG10205
ColiBase:b2478
Kegg Gene:b2478
EchoBASE ID:EB0201
CCDB:DAPA_ECOLI
BacMap:16130403
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Blickling, S., Renner, C., Laber, B., Pohlenz, H. D., Holak, T. A., Huber, R. (1997). "Reaction mechanism of Escherichia coli dihydrodipicolinate synthase investigated by X-ray crystallography and NMR spectroscopy." Biochemistry 36:24-33. Pubmed: 8993314
  • Bouvier, J., Pugsley, A. P., Stragier, P. (1991). "A gene for a new lipoprotein in the dapA-purC interval of the Escherichia coli chromosome." J Bacteriol 173:5523-5531. Pubmed: 1885529
  • Dobson, R. C., Devenish, S. R., Turner, L. A., Clifford, V. R., Pearce, F. G., Jameson, G. B., Gerrard, J. A. (2005). "Role of arginine 138 in the catalysis and regulation of Escherichia coli dihydrodipicolinate synthase." Biochemistry 44:13007-13013. Pubmed: 16185069
  • Dobson, R. C., Griffin, M. D., Jameson, G. B., Gerrard, J. A. (2005). "The crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from Escherichia coli with improved resolution show new features of biological significance." Acta Crystallogr D Biol Crystallogr 61:1116-1124. Pubmed: 16041077
  • Dobson, R. C., Valegard, K., Gerrard, J. A. (2004). "The crystal structure of three site-directed mutants of Escherichia coli dihydrodipicolinate synthase: further evidence for a catalytic triad." J Mol Biol 338:329-339. Pubmed: 15066435
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Laber, B., Gomis-Ruth, F. X., Romao, M. J., Huber, R. (1992). "Escherichia coli dihydrodipicolinate synthase. Identification of the active site and crystallization." Biochem J 288 ( Pt 2):691-695. Pubmed: 1463470
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Mirwaldt, C., Korndorfer, I., Huber, R. (1995). "The crystal structure of dihydrodipicolinate synthase from Escherichia coli at 2.5 A resolution." J Mol Biol 246:227-239. Pubmed: 7853400
  • Richaud, F., Richaud, C., Ratet, P., Patte, J. C. (1986). "Chromosomal location and nucleotide sequence of the Escherichia coli dapA gene." J Bacteriol 166:297-300. Pubmed: 3514578
  • Tiedeman, A. A., DeMarini, D. J., Parker, J., Smith, J. M. (1990). "DNA sequence of the purC gene encoding 5'-phosphoribosyl-5-aminoimidazole-4-N-succinocarboxamide synthetase and organization of the dapA-purC region of Escherichia coli K-12." J Bacteriol 172:6035-6041. Pubmed: 2120198
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837