D-alanine--D-alanine ligase A (P0A6J8)

Identification
Name:D-alanine--D-alanine ligase A
Synonyms:
  • D-Ala-D-Ala ligase A
  • D-alanylalanine synthetase A
Gene Name:ddlA
Enzyme Class:
Biological Properties
General Function:Involved in ATP binding
Specific Function:Cell wall formation
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
2.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
2.0D-Alanine + 1.0Adenosine triphosphate ↔ 1.0ADP + 1.0D-Alanyl-D-alanine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+2.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 2.0D-Alanine ↔ 1.0ADP + 1.0Phosphate + 1.0D-Alanyl-D-alanine
ReactionCard
SMPDB Reactions:
2.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
2.0D-Alanine + 1.0Adenosine triphosphate → 1.0D-Alanyl-D-alanine + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0ADP
ReactionCard
EcoCyc Reactions:
2.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
2.0D-Alanine + 1.0Adenosine triphosphate ↔ 1.0ADP + 1.0D-Alanyl-D-alanine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Alanine + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0D-Alanyl-D-alanine + 1.0Phosphate + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+2.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 2.0D-Alanine → 1.0ADP + 1.0Inorganic phosphate + 1.0D-Alanyl-D-alanine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01310D-AlanineMetaboCard
ECMDB03459D-Alanyl-D-alanineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Component
cell part
cell wall
cytoplasm
external encapsulating structure
intracellular part
Function
acid-amino acid ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
D-alanine-D-alanine ligase activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
nucleoside binding
purine nucleoside binding
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b0381
Gene OrientationCounterclockwise
Centisome Percentage:8.60
Left Sequence End399053
Right Sequence End400147
Gene Sequence:
>1095 bp
ATGTCGCGCGTCAATCCTCTATCTTCGCTGTCATTGTTGGCGGTTCTGGTTTTAGCGGGG
TGTAGTAGTCAGGCTCCGCAACCGTTGAAAAAGGGCGAAAAAGCGATAGATGTAGCGAGC
GTCGTGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTC
GCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTG
GCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAG
ATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTT
CATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCG
GTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACGATCTGATCAACATGGTGCCAATG
GGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGC
ATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGT
CAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTAT
CCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCC
AGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTG
GACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGC
AAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGT
GACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACG
AAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAA
ATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGT
TGTATGAAACAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:364
Protein Molecular Weight:39315
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>D-alanine--D-alanine ligase A
MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGIDKQGQWHVSDASNYLLN
ADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVIFPIVHGTLGEDGSLQGML
RVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFITLTRANRHNISFAEVESKLGL
PLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKVIVEQGIKGREIECAVLGNDNPQ
ASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAPEINDKIRAIAVQAYQTLGCAGMAR
VDVFLTPENEVVINEINTLPGFTNISMYPKLWQASGLGYTDLITRLIELALERHAADNAL
KTTM
References
External Links:
ResourceLink
Uniprot ID:P0A6J8
Uniprot Name:DDLA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674519
Ecogene ID:EG10213
Ecocyc:EG10213
ColiBase:b0381
Kegg Gene:b0381
EchoBASE ID:EB0209
CCDB:DDLA_ECOLI
BacMap:16128366
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Zawadzke, L. E., Bugg, T. D., Walsh, C. T. (1991). "Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes." Biochemistry 30:1673-1682. Pubmed: 1993184