ECMDB: D-amino acid dehydrogenase small subunit (P0A6J5)

Identification

Name:

D-amino acid dehydrogenase small subunit

Synonyms:

Not Available

Gene Name:

dadA

Enzyme Class:

1.4.99.1

Biological Properties

General Function:

Involved in D-amino-acid dehydrogenase activity

Specific Function:

Oxidative deamination of D-amino acids

Cellular Location:

Cell inner membrane; Peripheral membrane protein

SMPDB Pathways:

D-Alanine metabolism PW000768
L-alanine metabolism PW000788
Nitrogen metabolism PW000755
Phenylalanine metabolism PW000921

KEGG Pathways:

D-Alanine metabolism ec00473
Nitrogen metabolism ec00910
Phenylalanine metabolism ec00360

KEGG Reactions:

1.0alpha-Amino acid

1.0

1.0Acceptor

↔

1.02-Oxo acid

1.0

1.0Reduced acceptor

1.0alpha-Amino acid + 1.0Water + 1.0Acceptor ↔ 1.02-Oxo acid + 1.0Ammonia + 1.0Reduced acceptor
ReactionCard

1.0

1.0Acceptor

↔

1.0

1.0Reduced acceptor

1.0D-Phenylalanine + 1.0Water + 1.0Acceptor ↔ 1.0Phenylpyruvic acid + 1.0Ammonia + 1.0Reduced acceptor
ReactionCard

1.0D-Amino acid

1.0

1.0Acceptor

↔

1.02-Oxo acid

1.0

1.0Reduced acceptor

1.0D-Amino acid + 1.0Water + 1.0Acceptor ↔ 1.02-Oxo acid + 1.0Ammonia + 1.0Reduced acceptor
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0D-Alanine + 1.0Water + 1.0Quinone → 1.0Ammonium + 1.0Pyruvic acid + 1.0Hydroquinone
ReactionCard

1.0

1.0cytochrome c nitrite reductase

↔

1.0

1.0cytochrome c nitrite reductase

1.0Phenylpyruvic acid + 1.0Ammonia + 1.0cytochrome c nitrite reductase ↔ 1.0D-Phenylalanine + 1.0Water + 1.0cytochrome c nitrite reductase
ReactionCard

1.0

1.0an electron-transfer quinone

→

1.0

1.0electron-transfer quinol

1.0D-Alanine + 1.0Water + 1.0an electron-transfer quinone → 1.0Ammonium + 1.0Pyruvic acid + 1.0electron-transfer quinol
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0D-Alanine + 1.0FAD + 1.0Water → 1.0FADH2 + 1.0Ammonium + 1.0Pyruvic acid
ReactionCard

1.0A D-amino acid

1.0

1.0acceptor

→

1.0a 2-oxo acid

1.0

1.0reduced acceptor

1.0A D-amino acid + 1.0Water + 1.0acceptor → 1.0a 2-oxo acid + 1.0Ammonia + 1.0reduced acceptor
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00051	Ammonia	MetaboCard
ECMDB21186	Ammonium	MetaboCard
ECMDB01310	D-Alanine	MetaboCard
ECMDB20144	D-Phenylalanine	MetaboCard
ECMDB01248	FAD	MetaboCard
ECMDB01197	FADH2	MetaboCard
ECMDB02434	Hydroquinone	MetaboCard
ECMDB00205	Phenylpyruvic acid	MetaboCard
ECMDB00243	Pyruvic acid	MetaboCard
ECMDB23060	Quinone	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
oxidoreductase activity

Gene Properties

Blattner:

b1189

Gene Orientation

Clockwise

Centisome Percentage:

26.66

Left Sequence End

1236794

Right Sequence End

1238092

Gene Sequence:

>1299 bp
ATGACACTCACGCTCAATAGACAACTTCTCACCTCACGCCAGATTCTGGTGGCCTTTAGC
GGCGGGCTTGACTCCACCGTTCTGCTGCATCAGTTGGTGCAGTGGCGGACGGAAAATCCG
GGTGTCGCTCTGCGCGCTATTCATGTGCATCACGGTTTAAGTGCCAATGCCGATGCCTGG
GTTACGCATTGCGAAAACGTCTGCCAACAGTGGCAGGTGCCGCTGGTGGTCGAACGCGTA
CAACTTGCGCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCA
TTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAA
TGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATG
GCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGG
GGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAAT
CAGGACGACTCATACGATCGTAACTTTCTGCGCCTGCGCGTAGTGCCGTTATTGCAGCAG
CGTTGGCCGCATTTTGCCGAAGCAACGGCCCGCAGCGCCGCACTTTGTGCTGAACAAGAG
AGCCTGCTGGATGAACTGCTGGCAGATGATTTAGCACACTGTCAATCGCCGCAGGGGACG
CTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGC
TGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAG
GAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGCGCGTTTGAAATC
CGACGCTATCAGTCGCAACTGTGGTGGATTAAATCCGTCACCGGGCAAAGCGAAAACATT
GTGCCGTGGCAGACGTGGCTTCAACCGCTGGAATTACCGGCGGGGCTGGGAAGTGTACAG
CTTAATGCGGGAGGCGATATTCGCCCTCCGCGTGCAGACGAAGCGGTCAGCGTGCGTTTC
AAAGCGCCAGGATTGCTGCATATTGTCGGGCGTAACGGCGGACGTAAGCTAAAGAAAATC
TGGCAAGAGCTGGGCGTGCCGCCGTGGCTACGTGACACCACGCCACTGCTGTTTTATGGC
GAAACGCTGATTGCGGCGGCAGGGGTATTTGTGACGCAAGAAGGTGTGGCTGAAGGTGAG
AATGGCGTAAGTTTTGTCTGGCAGAAAACGCTTAGTTAA

Protein Properties

Pfam Domain Function:

DAO (PF01266 )

Protein Residues:

432

Protein Molecular Weight:

47607

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>D-amino acid dehydrogenase small subunit
MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGV
PLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLK
ALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPALA
EVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCG
DEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIA
ITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPD
GTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYSRGFT
PSRPGHLHGAHS

References

External Links:

Resource	Link
Uniprot ID:	P0A6J5
Uniprot Name:	DADA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4902929
Ecogene ID:	EG11407
Ecocyc:	EG11407
ColiBase:	b1189
Kegg Gene:	b1189
EchoBASE ID:	EB1379
CCDB:	DADA_ECOLI
BacMap:	16129152

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Lobocka, M., Hennig, J., Wild, J., Klopotowski, T. (1994). "Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of D-amino acid dehydrogenase and the catabolic alanine racemase." J Bacteriol 176:1500-1510. Pubmed: 7906689
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232