Adenylyl-sulfate kinase (P0A6J1)

Identification
Name:Adenylyl-sulfate kinase
Synonyms:
  • APS kinase
  • ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
  • Adenosine-5'-phosphosulfate kinase
Gene Name:cysC
Enzyme Class:
Biological Properties
General Function:Involved in adenylylsulfate kinase activity
Specific Function:Catalyzes the synthesis of activated sulfate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine phosphosulfate + 1.0Adenosine triphosphate ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Phosphoadenosine phosphosulfate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Adenosine phosphosulfate ↔ 1.0ADP + 1.0Phosphoadenosine phosphosulfate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Adenylylselenate ↔ 1.0ADP + 1.03'-Phosphoadenylylselenate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0Adenosine phosphosulfate + 1.0Adenosine triphosphate → 1.0Phosphoadenosine phosphosulfate + 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine phosphosulfate + 1.0Adenosine triphosphate ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Phosphoadenosine phosphosulfate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Adenosine phosphosulfate → 1.0ADP + 1.0Phosphoadenosine phosphosulfate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200803'-PhosphoadenylylselenateMetaboCard
ECMDB01003Adenosine phosphosulfateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB21003AdenylylselenateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01134Phosphoadenosine phosphosulfateMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
nucleoside binding
purine nucleoside binding
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular metabolic process
metabolic process
sulfate assimilation
sulfur metabolic process
Gene Properties
Blattner:b2750
Gene OrientationCounterclockwise
Centisome Percentage:61.89
Left Sequence End2871409
Right Sequence End2872014
Gene Sequence:
>606 bp
ATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGAT
AAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCGATCGTCAGGGGAAA
ACGGCAATTACGCTGGCAAGTTTATATCAGCAATATGCTTGCGTTCAGGCATTAATTGAT
GCCGGAGCGGATATTAATAAGCAAGATCATACCTGTTTAAATCCTTTTTTAATTAGCTGT
CTGAACGATGATTTAACGCTACTACGAATTATTTTACCGGCTAAACCCGATCTTAATTGC
GTAACCCGTTTTGGCGGTGTCGGCCTGACGCCTGCCTGTGAAAAAGGCCATTTAAGTATT
GTAAAAGAGCTTTTGGCGCATACGGAGATTAACGTTAACCAGACCAACCATGTAGGCTGG
ACGCCGCTACTGGAAGCGATTGTGCTTAATGATGGTGGTATTAAACAGCAGGCGATTGTG
CAGTTATTGCTGGAACACGGTGCCAGCCCGCATCTGACCGATAAATATGGCAAAACGCCA
CTGGAACTGGCGCGGGAACGGGGCTTTGAAGAGATTGCGCAGTTACTGATTGCCGCAGGT
GCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:201
Protein Molecular Weight:22321
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Adenylyl-sulfate kinase
MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTVAGALEEALHKLGVSTY
LLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLVVLTAFISPHRAERQMVRE
RVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFTGIDSVYEAPESAEIHLNGEQ
LVTNLVQQLLDLLRQNDIIRS
References
External Links:
ResourceLink
Uniprot ID:P0A6J1
Uniprot Name:CYSC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674461
Ecogene ID:EG10185
Ecocyc:EG10185
ColiBase:b2750
Kegg Gene:b2750
EchoBASE ID:EB0182
CCDB:CYSC_ECOLI
BacMap:16130657
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Leyh, T. S., Taylor, J. C., Markham, G. D. (1988). "The sulfate activation locus of Escherichia coli K12: cloning, genetic, and enzymatic characterization." J Biol Chem 263:2409-2416. Pubmed: 2828368
  • Leyh, T. S., Vogt, T. F., Suo, Y. (1992). "The DNA sequence of the sulfate activation locus from Escherichia coli K-12." J Biol Chem 267:10405-10410. Pubmed: 1316900
  • Satishchandran, C., Hickman, Y. N., Markham, G. D. (1992). "Characterization of the phosphorylated enzyme intermediate formed in the adenosine 5'-phosphosulfate kinase reaction." Biochemistry 31:11684-11688. Pubmed: 1332767