Putative dethiobiotin synthetase (P0A6E9)

Identification
Name:Putative dethiobiotin synthetase
Synonyms:
  • DTB synthetase
  • DTBS
  • Dethiobiotin synthase
Gene Name:ynfK
Enzyme Class:
Biological Properties
General Function:Involved in magnesium ion binding
Specific Function:ATP + 7,8-diaminononanoate + CO(2) = ADP + phosphate + dethiobiotin
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+3.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Carbon dioxide + 1.07,8-Diaminononanoate ↔ 1.0ADP + 1.0Dethiobiotin + 3.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.07,8-Diaminononanoate + 1.0Carbon dioxide ↔ 1.0ADP + 1.0Phosphate + 1.0Dethiobiotin
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+3.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Carbon dioxide + 1.07,8-Diaminononanoate ↔ 1.0ADP + 1.0Dethiobiotin + 3.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB201097,8-DiaminononanoateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB04030Carbon dioxideMetaboCard
ECMDB03581DethiobiotinMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
cation binding
cyclo-ligase activity
dethiobiotin synthase activity
ion binding
ligase activity
ligase activity, forming carbon-nitrogen bonds
magnesium ion binding
metal ion binding
nucleoside binding
purine nucleoside binding
Process
biotin biosynthetic process
biotin metabolic process
metabolic process
small molecule metabolic process
vitamin metabolic process
water-soluble vitamin metabolic process
Gene Properties
Blattner:b1593
Gene OrientationCounterclockwise
Centisome Percentage:35.88
Left Sequence End1664548
Right Sequence End1665243
Gene Sequence:
>696 bp
ATGGATAATCGAGGCGAATTTTTGAATAACGTTGCTCAGGCACTGGGTCGCCCGCTGCGA
CTTGAACCGCAAGCAGAAGATGCGCCGCTTAACAACTATGCTAACGAGCGGCTTACCCAA
CTTAACCAACAGCAGCGCTGTGACGCGTTTATTCAGTTTGCCAGCGATGTTATGTTGACG
CGCTGTGAGCTGACCAGCGAGGCGAAGGCGGCAGAAGCTGCAATACGTCTGTGTAAAGAG
CTGGGAGATCAGTCGGTCGTGATTAGCGGTGACACGAGGCTGGAGGAATTGGGGATTAGC
GAACGTTTGCAGCAGGAATGCAATGCCGTTGTTTGGGATCCGGCGAAAGGTGCCGAGAAT
ATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAA
TCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCG
GAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCA
GAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAACATCATT
AGCGGCCCCAGTTCAACGGCGGATATTGAGCTTATCAAAGTCGTCGGAGTTCATGGCCCG
GTGAAAGCGGTGTATCTGATTATTGAGGATTGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:231
Protein Molecular Weight:24981
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Putative dethiobiotin synthetase
MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGSKETPEGLRNKDALVLQ
SVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANLTEKVDHVVVEGTGGWRSL
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
YAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV
References
External Links:
ResourceLink
Uniprot ID:P0A6E9
Uniprot Name:BIOD2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674452
Ecogene ID:EG13849
Ecocyc:EG13849
ColiBase:b1593
Kegg Gene:b1593
EchoBASE ID:EB3610
CCDB:BIOD2_ECOLI
BacMap:90111304
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553