Shikimate kinase 2 (P0A6E1)

Identification
Name:Shikimate kinase 2
Synonyms:
  • SK 2
  • Shikimate kinase II
  • SKII
Gene Name:aroL
Enzyme Class:
Biological Properties
General Function:Involved in shikimate kinase activity
Specific Function:Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
  • Metabolic pathways eco01100
  • Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Shikimic acid ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Shikimate 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Shikimic acid ↔ 1.0ADP + 1.0Shikimate 3-phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0shikimate 3-phosphate+1.0Thumb+1.0Thumb
1.0Shikimic acid + 1.0Adenosine triphosphate → 1.0Adenosine diphosphate + 1.0Hydrogen ion + 1.0shikimate 3-phosphate + 1.0ADP + 1.0Shikimate 3-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Shikimic acid ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Shikimate 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Shikimic acid + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Shikimate 3-phosphate + 1.0ADP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20193Shikimate 3-phosphateMetaboCard
ECMDB03070Shikimic acidMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
nucleoside binding
purine nucleoside binding
shikimate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Gene Properties
Blattner:b0388
Gene OrientationClockwise
Centisome Percentage:8.74
Left Sequence End405629
Right Sequence End406153
Gene Sequence:
>525 bp
ATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATG
GCCCTTGCCGATTCGCTTAACCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAG
CTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGTGGGCGGGATTTCGCGCCAGA
GAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCACCGTTATCGCTACAGGCGGCGGC
ATTATTCTGACGGAATTTAATCGTCACTTCATGCAAAATAACGGGATCGTGGTTTATTTG
TGTGCGCCAGTATCAGTCCTGGTTAACCGACTGCAAGCTGCACCGGAAGAAGATTTACGG
CCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGGAAGTGCTGGAAGAACGCGAT
GCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTG
ATTTCTGAAATTCGCAGCGCCCTGGCACAGACGATCAATTGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:174
Protein Molecular Weight:19151
Protein Theoretical pI:4
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Shikimate kinase 2
MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRAR
ETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLR
PTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSALAQTINC
References
External Links:
ResourceLink
Uniprot ID:P0A6E1
Uniprot Name:AROL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674529
Ecogene ID:EG10082
Ecocyc:EG10082
ColiBase:b0388
Kegg Gene:b0388
EchoBASE ID:EB0080
CCDB:AROL_ECOLI
BacMap:16128373
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • DeFeyter, R. C., Davidson, B. E., Pittard, J. (1986). "Nucleotide sequence of the transcription unit containing the aroL and aroM genes from Escherichia coli K-12." J Bacteriol 165:233-239. Pubmed: 3001025
  • DeFeyter, R. C., Pittard, J. (1986). "Genetic and molecular analysis of aroL, the gene for shikimate kinase II in Escherichia coli K-12." J Bacteriol 165:226-232. Pubmed: 3001024
  • DeFeyter, R. C., Pittard, J. (1986). "Purification and properties of shikimate kinase II from Escherichia coli K-12." J Bacteriol 165:331-333. Pubmed: 3001029
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Heatwole, V. M., Somerville, R. L. (1992). "Synergism between the Trp repressor and Tyr repressor in repression of the aroL promoter of Escherichia coli K-12." J Bacteriol 174:331-335. Pubmed: 1530846
  • Lawley, B., Pittard, A. J. (1994). "Regulation of aroL expression by TyrR protein and Trp repressor in Escherichia coli K-12." J Bacteriol 176:6921-6930. Pubmed: 7961453
  • Millar, G., Lewendon, A., Hunter, M. G., Coggins, J. R. (1986). "The cloning and expression of the aroL gene from Escherichia coli K12. Purification and complete amino acid sequence of shikimate kinase II, the aroL-gene product." Biochem J 237:427-437. Pubmed: 3026317