Shikimate kinase 1 (P0A6D7)

Identification
Name:Shikimate kinase 1
Synonyms:
  • SK 1
  • Shikimate kinase I
  • SKI
Gene Name:aroK
Enzyme Class:
Biological Properties
General Function:Involved in shikimate kinase activity
Specific Function:Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:Not Available
KEGG Pathways:
  • Metabolic pathways eco01100
  • Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Shikimic acid ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Shikimate 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Shikimic acid ↔ 1.0ADP + 1.0Shikimate 3-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Shikimic acid ↔ 1.0ADP + 1.0Hydrogen ion + 1.0Shikimate 3-phosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Shikimic acid + 1.0Adenosine triphosphate → 1.0Hydrogen ion + 1.0Shikimate 3-phosphate + 1.0ADP
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Shikimic acid → 1.0ADP + 1.0Shikimate 3-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20193Shikimate 3-phosphateMetaboCard
ECMDB03070Shikimic acidMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
nucleoside binding
purine nucleoside binding
shikimate kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Gene Properties
Blattner:b3390
Gene OrientationCounterclockwise
Centisome Percentage:75.79
Left Sequence End3516565
Right Sequence End3517086
Gene Sequence:
>522 bp
ATGCAACTGAACAAAGTGCTGAAAGGGCTGATGATTGCTCTGCCTGTTATGGCAATTGCG
GCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGC
ACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTG
CAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATC
CGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCT
TACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCC
CTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCA
GACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAA
GCGGCATACTCCAAAAACCGTCGTGCGGTACTGGTTTACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:173
Protein Molecular Weight:19538
Protein Theoretical pI:5
PDB File:1KAG
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Shikimate kinase 1
MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTGADVGWVFDLEGEEGFR
DREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVYLETTIEKQLARTQRDKKR
PLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAKVVANQIIHMLESN
References
External Links:
ResourceLink
Uniprot ID:P0A6D7
Uniprot Name:AROK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651333
PDB ID:1KAG
Ecogene ID:EG10081
Ecocyc:EG10081
ColiBase:b3390
Kegg Gene:b3390
EchoBASE ID:EB0079
CCDB:AROK_ECOLI
BacMap:90111581
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • DeFeyter, R. C., Pittard, J. (1986). "Purification and properties of shikimate kinase II from Escherichia coli K-12." J Bacteriol 165:331-333. Pubmed: 3001029
  • Griffin, H. G., Gasson, M. J. (1995). "The gene (aroK) encoding shikimate kinase I from Escherichia coli." DNA Seq 5:195-197. Pubmed: 7612934
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Lobner-Olesen, A., Boye, E., Marinus, M. G. (1992). "Expression of the Escherichia coli dam gene." Mol Microbiol 6:1841-1851. Pubmed: 1630320
  • Lobner-Olesen, A., Marinus, M. G. (1992). "Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli." J Bacteriol 174:525-529. Pubmed: 1309529
  • Lyngstadaas, A., Lobner-Olesen, A., Boye, E. (1995). "Characterization of three genes in the dam-containing operon of Escherichia coli." Mol Gen Genet 247:546-554. Pubmed: 7603433
  • Romanowski, M. J., Burley, S. K. (2002). "Crystal structure of the Escherichia coli shikimate kinase I (AroK) that confers sensitivity to mecillinam." Proteins 47:558-562. Pubmed: 12001235
  • Whipp, M. J., Pittard, A. J. (1995). "A reassessment of the relationship between aroK- and aroL-encoded shikimate kinase enzymes of Escherichia coli." J Bacteriol 177:1627-1629. Pubmed: 7883721
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841