Identification
Name:Amino-acid acetyltransferase
Synonyms:
  • N-acetylglutamate synthase
  • AGS
  • NAGS
Gene Name:argA
Enzyme Class:
Biological Properties
General Function:Involved in cellular amino acid biosynthetic process
Specific Function:Acetyl-CoA + L-glutamate = CoA + N-acetyl-L- glutamate
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
SMPDB Reactions:
1.0Thumb+1.0L-Glutamic acid+1.0Thumb1.0N-Acetyl-L-alanine+1.0Thumb+1.0Thumb+1.0N-Acetylglutamic acid+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0L-Glutamic acid + 1.0L-Glutamate ↔ 1.0N-Acetyl-L-alanine + 1.0Coenzyme A + 1.0Hydrogen ion + 1.0N-Acetylglutamic acid + 1.0N-Acetyl-L-alanine + 1.0N-Acetylglutamic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01206Acetyl-CoAMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB01138N-Acetyl-L-alanineMetaboCard
ECMDB21429N-Acetylglutamic acidMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
acetyl-CoA:L-glutamate N-acetyltransferase activity
acetyltransferase activity
acyltransferase activity
catalytic activity
N-acetyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
arginine biosynthetic process
arginine metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
metabolic process
Gene Properties
Blattner:b2818
Gene OrientationClockwise
Centisome Percentage:63.52
Left Sequence End2947264
Right Sequence End2948595
Gene Sequence:
>1332 bp
ATGGTAAAGGAACGTAAAACCGAGTTGGTCGAGGGATTCCGCCATTCGGTTCCCTATATC
AATACCCACCGGGGAAAAACGTTTGTCATCATGCTCGGCGGTGAAGCCATTGAGCATGAG
AATTTCTCCAGTATCGTTAATGATATCGGGTTGTTGCACAGCCTCGGCATCCATCTGGTG
GTGGTCTATGGCGCACGTCCGCAGATTGACGCAAATCTGGCTGCGCATCACCACGAACCG
CTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCT
GCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCG
CTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGC
GTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCG
ATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTC
ACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTG
AAAGCTGAAAAGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGAT
ATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAA
GGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGC
GGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTG
TTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAGCGCCGAGCAGATTCGTCGC
GCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGT
ATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATT
CAGCGCGATAACACGACTATTGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATT
GGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCAGTTCATCAAGGGGTGAAGTT
CTGCTGGAACGCATTGCCGCTCAGGCGAAGCAGAGCGGCTTAAGCAAATTGTTTGTGCTG
ACCACGCGCAGTATTCACTGGTTCCAGGAACGTGGATTTACCCCAGTGGATATTGATTTA
CTGCCCGAGAGCAAAAAGCAGTTGTACAACTACCAGCGTAAATCCAAAGTGTTGATGGCG
GATTTAGGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:443
Protein Molecular Weight:49195
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Amino-acid acetyltransferase
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLV
VVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTP
LQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDAIHRQLDSGAIVLMGPVAVSV
TGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRR
ATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKI
GEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVLTTRSIHWFQERGFTPVDIDL
LPESKKQLYNYQRKSKVLMADLG
References
External Links:
ResourceLink
Uniprot ID:P0A6C5
Uniprot Name:ARGA_ECOLI
GenBank Gene ID:AF008115
Genebank Protein ID:3128247
Ecogene ID:EG10063
Ecocyc:EG10063
ColiBase:b2818
Kegg Gene:b2818
EchoBASE ID:EB0061
CCDB:ARGA_ECOLI
BacMap:16130722
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Brown, K., Finch, P. W., Hickson, I. D., Emmerson, P. T. (1987). "Complete nucleotide sequence of the Escherichia coli argA gene." Nucleic Acids Res 15:10586. Pubmed: 3320971
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Marvil, D. K., Leisinger, T. (1977). "N-acetylglutamate synthase of Escherichia coli: purification, characterization, and molecular properties." J Biol Chem 252:3295-3303. Pubmed: 16890
  • Rajagopal, B. S., DePonte, J. 3rd, Tuchman, M., Malamy, M. H. (1998). "Use of inducible feedback-resistant N-acetylglutamate synthetase (argA) genes for enhanced arginine biosynthesis by genetically engineered Escherichia coli K-12 strains." Appl Environ Microbiol 64:1805-1811. Pubmed: 9572954