Identification
Name:Probable ubiquinone biosynthesis protein ubiB
Synonyms:Not Available
Gene Name:ubiB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transferase activity, transferring phosphorus-containing groups
Specific Function:Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis. Specific for aerobically grown log-phase cells
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
1.02-Polyprenylphenol+1.0Thumb+1.0Thumb1.02-Polyprenyl-6-hydroxyphenol+1.0Thumb+1.0Thumb
1.02-Polyprenylphenol + 1.0Oxygen + 1.0NADPH ↔ 1.02-Polyprenyl-6-hydroxyphenol + 1.0NADP + 1.0Water
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0Thumb+0.5Thumb1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB041172-Octaprenyl-6-hydroxyphenolMetaboCard
ECMDB041202-OctaprenylphenolMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB20487Ubiquinone-0MetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
oxidoreduction coenzyme metabolic process
ubiquinone biosynthetic process
ubiquinone metabolic process
Gene Properties
Blattner:b3835
Gene OrientationClockwise
Centisome Percentage:86.61
Left Sequence End4018249
Right Sequence End4019889
Gene Sequence:
>1641 bp
ATGGCAATCCACAATCGTGCAGGCCAACCTGCACAACAGAGTGATTTGATTAACGTCGCC
CAACTGACGGCGCAATATTATGTACTGAAACCAGAAGCAGGGAATGCGGAGCACGCGGTG
AAATTCGGTACTTCCGGTCACCGTGGCAGTGCAGCGCGCCACAGCTTTAACGAGCCGCAC
ATTCTGGCGATCGCTCAGGCAATTGCTGAAGAACGTGCGAAAAACGGCATCACTGGCCCT
TGCTATGTGGGTAAAGATACTCACGCCCTGTCCGAACCTGCATTCATTTCCGTTCTGGAA
GTGCTGGCAGCGAACGGCGTTGATGTCATTGTGCAGGAAAACAATGGCTTCACCCCGACG
CCTGCCGTTTCCAATGCCATCCTGGTTCACAATAAAAAAGGTGGCCCGCTGGCAGACGGT
ATCGTGATTACACCGTCCCATAACCCGCCGGAAGATGGTGGAATCAAATACAATCCGCCA
AATGGTGGCCCGGCTGATACCAACGTCACTAAAGTGGTGGAAGACAGGGCCAACGCACTG
CTGGCCGATGGCCTGAAAGGCGTGAAGCGTATCTCCCTCGACGAAGCGATGGCATCCGGT
CATGTGAAAGAGCAGGATCTGGTGCAGCCGTTCGTGGAAGGTCTGGCCGATATCGTTGAT
ATGGCCGCGATTCAGAAAGCGGGCCTGACGCTGGGCGTTGATCCGCTGGGCGGTTCCGGT
ATCGAATACTGGAAGCGTATTGGCGAGTATTACAACCTCAACCTGACTATCGTTAACGAT
CAGGTCGATCAAACCTTCCGCTTTATGCACCTTGATAAAGACGGCGCGATCCGTATGGAC
TGCTCCTCCGAGTGTGCGATGGCGGGCCTGCTGGCACTGCGTGATAAGTTCGATCTGGCG
TTTGCTAACGACCCGGATTATGACCGTCACGGTATCGTCACTCCGGCAGGTTTGATGAAT
CCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGC
AAAGATGTTGCCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAAC
GACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTG
TTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGGGGCTTCCTTCCTGCGTTTC
GACGGCACGCCGTGGTCCACCGACAAAGACGGCATCATCATGTGTCTGCTGGCGGCGGAA
ATCACCGCTGTCACCGGTAAGAACCCGCAGGAACACTACAACGAACTGGCAAAACGCTTT
GGTGCGCCGAGCTACAACCGTTTGCAGGCAGCTGCGACTTCCGCACAAAAAGCGGCGCTG
TCTAAGCTGTCTCCGGAAATGGTGAGCGCCAGCACCCTGGCAGGTGACCCGATCACCGCG
CGCCTGACTGCTGCTCCGGGCAACGGTGCTTCTATTGGCGGTCTGAAAGTGATGACTGAC
AACGGCTGGTTCGCCGCGCGTCCGTCAGGCACGGAAGACGCATATAAGATCTACTGCGAA
AGCTTCCTCGGTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGATTGTTAGC
GAAGTTCTGAAAAACGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:546
Protein Molecular Weight:63202
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 501-521
  • 522-542
Protein Sequence:
>Probable ubiquinone biosynthesis protein ubiB
MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWMPNRHKDKLLGERLRLA
LQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDGKLAKQQIEAAMGGLPVEA
WFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDILPVIKADLKLIYRLARWVPRL
LPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRRNFEDSPMLYIPEVYPDYCSEGM
MVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFFTQVFRDSFFHADMHPGNIFVSYEH
PENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFNRDYRKVAELHVDSGWVPPDTNVEEFE
FAIRTVCEPIFEKPLAEISFGHVLLNLFNTARRFNMEVQPQLVLLQKTLLYVEGVGRQLY
PQLDLWKTAKPFLESWIKDQVGIPALVRAFKEKAPFWVEKMPELPELVYDSLRQGKYLQH
SVDKIARELQSNHVRQGQSRYFLGIGATLVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFV
GWRKTR
References
External Links:
ResourceLink
Uniprot ID:P0A6A0
Uniprot Name:UBIB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651293
Ecogene ID:EG11476
Ecocyc:EG11476
ColiBase:b3835
Kegg Gene:b3835
EchoBASE ID:EB1443
CCDB:UBIB_ECOLI
BacMap:16131684
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Macinga, D. R., Cook, G. M., Poole, R. K., Rather, P. N. (1998). "Identification and characterization of aarF, a locus required for production of ubiquinone in Providencia stuartii and Escherichia coli and for expression of 2'-N-acetyltransferase in P. stuartii." J Bacteriol 180:128-135. Pubmed: 9422602
  • Mizuno, T. (1987). "Random cloning of bent DNA segments from Escherichia coli chromosome and primary characterization of their structures." Nucleic Acids Res 15:6827-6841. Pubmed: 3309887
  • Poon, W. W., Davis, D. E., Ha, H. T., Jonassen, T., Rather, P. N., Clarke, C. F. (2000). "Identification of Escherichia coli ubiB, a gene required for the first monooxygenase step in ubiquinone biosynthesis." J Bacteriol 182:5139-5146. Pubmed: 10960098