Malate synthase A (P08997)

Identification
Name:Malate synthase A
Synonyms:
  • MSA
Gene Name:aceB
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Cellular Location:Cytoplasm
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Glyoxylic acid + 1.0Water ↔ 1.0Coenzyme A + 1.0Hydrogen ion + 1.0L-Malic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0L-Malic acid + 1.0Coenzyme A ↔ 1.0Acetyl-CoA + 1.0Water + 1.0Glyoxylic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Glyoxylic acid + 1.0Water ↔ 1.0Coenzyme A + 1.0Hydrogen ion + 1.0L-Malic acid
ReactionCard
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Acetyl-CoA + 1.0Water + 1.0Glyoxylic acid → 1.0Hydrogen ion + 1.0L-Malic acid + 1.0Coenzyme A
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01206Acetyl-CoAMetaboCard
ECMDB01423Coenzyme AMetaboCard
ECMDB00119Glyoxylic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00156L-Malic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
malate synthase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer
Process
carbohydrate metabolic process
cellular carbohydrate metabolic process
glyoxylate cycle
metabolic process
primary metabolic process
Gene Properties
Blattner:b4014
Gene OrientationClockwise
Centisome Percentage:90.81
Left Sequence End4213501
Right Sequence End4215102
Gene Sequence:
>1602 bp
ATGACTGAACAGGCAACAACAACCGATGAACTGGCTTTCACAAGGCCGTATGGCGAGCAG
GAGAAGCAAATTCTTACTGCCGAAGCGGTAGAATTTCTGACTGAGCTGGTGACGCATTTT
ACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAAC
GGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATT
CGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGC
AAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGAT
TCACTGGCACCAGACTGGAACAAAGTGATCGACGGGCAAATTAACCTGCGTGATGCGGTT
AACGGCACCATCAGTTACACCAATGAAGCAGGCAAAATTTACCAGCTCAAGCCCAATCCA
GCGGTTTTGATTTGTCGGGTACGCGGTCTGCACTTGCCGGAAAAACATGTCACCTGGCGT
GGTGAGGCAATCCCCGGCAGCCTGTTTGATTTTGCGCTCTATTTCTTCCACAACTATCAG
GCACTGTTGGCAAAGGGCAGTGGTCCCTATTTCTATCTGCCGAAAACCCAGTCCTGGCAG
GAAGCGGCCTGGTGGAGCGAAGTCTTCAGCTATGCAGAAGATCGCTTTAATCTGCCGCGC
GGCACCATCAAGGCGACGTTGCTGATTGAAACGCTGCCCGCCGTGTTCCAGATGGATGAA
ATCCTTCACGCGCTGCGTGACCATATTGTTGGTCTGAACTGCGGTCGTTGGGATTACATC
TTCAGCTATATCAAAACGTTGAAAAACTATCCCGATCGCGTCCTGCCAGACAGACAGGCA
GTGACGATGGATAAACCATTCCTGAATGCTTACTCACGCCTGTTGATTAAAACCTGCCAT
AAACGCGGTGCTTTTGCGATGGGCGGCATGGCGGCGTTTATTCCGAGCAAAGATGAAGAG
CACAATAACCAGGTGCTCAACAAAGTAAAAGCGGATAAATCGCTGGAAGCCAATAACGGT
CACGATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAAC
GACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATT
ACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCC
AACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCG
ATTTATGGCCTGATGGAAGATGCGGCGACGGCTGAAATTTCCCGTACCTCGATCTGGCAG
TGGATCCATCATCAAAAAACGTTGAGCAATGGCAAACCGGTGACCAAAGCCTTGTTCCGC
CAGATGCTGGGCGAAGAGATGAAAGTCATTGCCAGCGAACTGGGCGAAGAACGTTTCTCC
CAGGGGCGTTTTGACGATGCCGCACGCTTGATGGAACAGATCACCACTTCCGATGAGTTA
ATTGATTTCCTGACCCTGCCAGGCTACCGCCTGTTAGCGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:533
Protein Molecular Weight:60273
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Malate synthase A
MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQRNKLLAARIQQQQDIDN
GTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKMVINALNANVKVFMADFED
SLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNPAVLICRVRGLHLPEKHVTWR
GEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQSWQEAAWWSEVFSYAEDRFNLPR
GTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGRWDYIFSYIKTLKNYPDRVLPDRQA
VTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFIPSKDEEHNNQVLNKVKADKSLEANNG
HDGTWIAHPGLADTAMAVFNDILGSRKNQLEVMREQDAPITADQLLAPCDGERTEEGMRA
NIRVAVQYIEAWISGNGCVPIYGLMEDAATAEISRTSIWQWIHHQKTLSNGKPVTKALFR
QMLGEEMKVIASELGEERFSQGRFDDAARLMEQITTSDELIDFLTLPGYRLLA
References
External Links:
ResourceLink
Uniprot ID:P08997
Uniprot Name:MASY_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676766
Ecogene ID:EG10023
Ecocyc:EG10023
ColiBase:b4014
Kegg Gene:b4014
EchoBASE ID:EB0022
CCDB:MASY_ECOLI
BacMap:16131840
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Byrne, C., Stokes, H. W., Ward, K. A. (1988). "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli." Nucleic Acids Res 16:10924. Pubmed: 3060852
  • Byrne, C., Stokes, H. W., Ward, K. A. (1988). "Nucleotide sequence of the aceB gene encoding malate synthase A in Escherichia coli." Nucleic Acids Res 16:9342. Pubmed: 3050899
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553