Lysine-sensitive aspartokinase 3 (P08660)

Identification
Name:Lysine-sensitive aspartokinase 3
Synonyms:
  • Aspartate kinase III
  • AKIII
  • Lysine-sensitive aspartokinase III
Gene Name:lysC
Enzyme Class:
Biological Properties
General Function:Involved in amino acid binding
Specific Function:ATP + L-aspartate = ADP + 4-phospho-L- aspartate
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Aspartic acid + 1.0Adenosine triphosphate ↔ 1.0L-Aspartyl-4-phosphate + 1.0ADP
ReactionCard
SMPDB Reactions:
1.0L-Aspartic acid+1.0Thumb+1.0Thumb → 1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
1.0L-Aspartic acid + 1.0Adenosine triphosphate + 1.0L-Aspartic acid → 1.0Adenosine diphosphate + 1.0L-Aspartyl-4-phosphate + 1.0ADP
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Aspartic acid + 1.0Adenosine triphosphate ↔ 1.0L-Aspartyl-4-phosphate + 1.0ADP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB00191L-Aspartic acidMetaboCard
ECMDB12250L-Aspartyl-4-phosphateMetaboCard
GO Classification:
Function
amino acid binding
aspartate kinase activity
binding
carboxylic acid binding
catalytic activity
phosphotransferase activity, carboxyl group as acceptor
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b4024
Gene OrientationCounterclockwise
Centisome Percentage:91.17
Left Sequence End4229907
Right Sequence End4231256
Gene Sequence:
>1350 bp
ATGAAATTTACCCAACGTCTTAGTCTGCGCGTCAGGCTGACGCTAATCTTTTTAATTCTG
GCCTCGGTGACCTGGCTGCTTTCCAGCTTTGTCGCCTGGAAACAAACAACGGATAACGTC
GATGAATTGTTCGACACCCAACTGATGCTGTTTGCCAAGCGGTTAAGTACGCTCGATCTC
AACGAAATCAACGCGGCGGATCGCATGGCACAGACGCCAAATAGATTAAAACACGGTCAT
GTTGATGACGATGCGCTGACCTTTGCCATCTTTACCCACGACGGCAGAATGGTCCTTAAT
GATGGCGATAACGGAGAAGATATTCCCTATAGCTATCAACGGGAAGGTTTTGCTGACGGG
CAACTGGTCGGTGAAGACGATCCTTGGCGTTTTGTCTGGATGACCTCACCTGATGGCAAA
TATCGCATCGTTGTTGGCCAGGAATGGGAATACCGTGAAGACATGGCGCTGGCGATTGTT
GCCGGGCAATTGATCCCGTGGCTGGTCGCACTGCCGATTATGTTAATCATCATGATGGTA
CTACTGGGTCGTGAACTCGCGCCGCTGAACAAACTGGCGCTGGCACTACGTATGCGTGAC
CCTGACTCGGAAAAACCACTAAACGCGACTGGCGTACCCAGCGAAGTGCGGCCACTGGTT
GAGTCGCTAAATCAACTGTTCGCCCGCACACATGCGATGATGGTTCGTGAACGACGCTTT
ACCTCCGACGCAGCTCACGAACTTCGTAGCCCGTTAACGGCGCTGAAAGTGCAAACCGAA
GTTGCGCAGCTCTCTGACGATGATCCGCAGGCGCGGAAAAAAGCACTGCTCCAATTACAT
TCCGGGATCGATCGCGCTACTCGTCTGGTTGATCAACTGCTCACGCTATCGCGGCTGGAC
TCACTGGATAACCTTCAGGACGTCGCGGAGATCCCGCTTGAAGATCTCCTGCAATCGTCG
GTGATGGATATTTACCACACGGCGCAGCAGGCGAAAATTGACGTGCGACTGACACTCAAT
GCCCACAGCATCAAACGCACCGGGCAACCGCTATTGCTAAGTTTGTTGGTGCGAAATTTG
CTGGATAACGCCGTGCGCTACAGTCCACAGGGCAGCGTGGTAGACGTCACGCTGAATGCT
GATAATTTCATCGTGAGGGATAACGGCCCCGGTGTGACACCAGAGGCACTGGCGCGAATT
GGCGAACGCTTCTATCGCCCACCCGGACAAACCGCTACCGGCAGCGGGCTTGGGCTATCG
ATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAA
GGTGGATTTGAGGCGAAGGTAAGCTGGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:449
Protein Molecular Weight:48531
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lysine-sensitive aspartokinase 3
MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGITNLLVALAEGLEPGER
FEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAAALATSPALTDELVSHGEL
MSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIAALAELAALQLLPRLNEGLVI
TQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVDIWTDVPGIYTTDPRVVSAAKRI
DEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFVGSSKDPRAGGTLVCNKTENPPLFR
ALALRRNQTLLTLHSLNMLHSRGFLAEVFGILARHNISVDLITTSEVSVALTLDTTGSTS
TGDTLLTQSLLMELSALCRVEVEEGLALVALIGNDLSKACGVGKEVFGVLEPFNIRMICY
GASSHNLCFLVPGEDAEQVVQKLHSNLFE
References
External Links:
ResourceLink
Uniprot ID:P08660
Uniprot Name:AK3_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675829
Ecogene ID:EG10550
Ecocyc:EG10550
ColiBase:b4024
Kegg Gene:b4024
EchoBASE ID:EB0545
CCDB:AK3_ECOLI
BacMap:16131850
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cassan, M., Parsot, C., Cohen, G. N., Patte, J. C. (1986). "Nucleotide sequence of lysC gene encoding the lysine-sensitive aspartokinase III of Escherichia coli K12. Evolutionary pathway leading to three isofunctional enzymes." J Biol Chem 261:1052-1057. Pubmed: 3003049
  • Cassan, M., Ronceray, J., Patte, J. C. (1983). "Nucleotide sequence of the promoter region of the E. coli lysC gene." Nucleic Acids Res 11:6157-6166. Pubmed: 6312411
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kotaka, M., Ren, J., Lockyer, M., Hawkins, A. R., Stammers, D. K. (2006). "Structures of R- and T-state Escherichia coli aspartokinase III. Mechanisms of the allosteric transition and inhibition by lysine." J Biol Chem 281:31544-31552. Pubmed: 16905770
  • Marco-Marin, C., Ramon-Maiques, S., Tavarez, S., Rubio, V. (2003). "Site-directed mutagenesis of Escherichia coli acetylglutamate kinase and aspartokinase III probes the catalytic and substrate-binding mechanisms of these amino acid kinase family enzymes and allows three-dimensional modelling of aspartokinase." J Mol Biol 334:459-476. Pubmed: 14623187