Orotidine 5'-phosphate decarboxylase (P08244)

Identification
Name:Orotidine 5'-phosphate decarboxylase
Synonyms:
  • OMP decarboxylase
  • OMPDCase
  • OMPdecase
Gene Name:pyrF
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Orotidylic acid ↔ 1.0Carbon dioxide + 1.0Uridine 5'-monophosphate
ReactionCard
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Orotidylic acid ↔ 1.0Uridine 5'-monophosphate + 1.0Carbon dioxide
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Orotidylic acid ↔ 1.0Carbon dioxide + 1.0Uridine 5'-monophosphate
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Orotidylic acid → 1.0Uridine 5'-monophosphate + 1.0Carbon dioxide
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00218Orotidylic acidMetaboCard
ECMDB00288Uridine 5'-monophosphateMetaboCard
GO Classification:
Function
carbon-carbon lyase activity
carboxy-lyase activity
catalytic activity
lyase activity
orotidine-5'-phosphate decarboxylase activity
Process
'de novo' pyrimidine base biosynthetic process
'de novo' UMP biosynthetic process
cellular aromatic compound metabolic process
cellular metabolic process
cellular nitrogen compound metabolic process
metabolic process
nitrogen compound metabolic process
nucleobase metabolic process
nucleobase, nucleoside and nucleotide metabolic process
nucleobase, nucleoside, nucleotide and nucleic acid metabolic process
nucleoside phosphate metabolic process
nucleotide metabolic process
pyrimidine base biosynthetic process
pyrimidine base metabolic process
pyrimidine nucleoside monophosphate biosynthetic process
pyrimidine nucleotide biosynthetic process
pyrimidine nucleotide metabolic process
pyrimidine ribonucleoside monophosphate biosynthetic process
UMP biosynthetic process
Gene Properties
Blattner:b1281
Gene OrientationClockwise
Centisome Percentage:28.88
Left Sequence End1339945
Right Sequence End1340682
Gene Sequence:
>738 bp
ATGTATCCCGTCGACCTTCATATGCATACCGTTGCCAGCACACATGCATATAGCACATTA
AGTGATTACATTGCCCAGGCCAAACAAAAGGGCATTAAACTTTTTGCGATCACCGATCAT
GGCCCGGATATGGAAGATGCGCCGCATCACTGGCACTTCATTAACATGCGTATCTGGCCG
CGAGTGGTTGATGGGGTAGGGATCCTGCGCGGCATCGAAGCTAACATTAAAAATGTTGAT
GGTGAAATTGACTGCAGCGGTAAAATGTTTGACTCGCTGGATCTAATTATTGCCGGTTTT
CATGAGCCGGTTTTTGCGCCACATGACAAAGCGACCAATACACAAGCGATGATCGCCACT
ATCGCCAGCGGCAATGTGCATATAATAAGCCATCCCGGAAATCCCAAATATGAAATAGAT
GTGAAAGCCGTTGCTGAAGCAGCCGCGAAACATCAGGTGGCGCTGGAAATCAATAATTCC
TCATTTTTACACTCACGTAAGGGCAGTGAAGACAACTGTCGTGAGGTAGCCGCAGCGGTA
CGTGATGCTGGAGGTTGGGTGGCATTAGGCTCGGATTCTCACACCGCGTTTACCATGGGG
GAATTTGAAGAGTGTCTTAAAATCCTCGACGCGGTAGATTTTCCGCCAGAGCGCATTTTG
AATGTTTCTCCGCGCCGCTTACTGAACTTCCTTGAATCTCGCGGTATGGCACCGATTGCG
GAATTTGCAGACCTTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:245
Protein Molecular Weight:26350
Protein Theoretical pI:6
PDB File:1EIX
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Orotidine 5'-phosphate decarboxylase
MTLTASSSSRAVTNSPVVVALDYHNRDDALAFVDKIDPRDCRLKVGKEMFTLFGPQFVRE
LQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASGGARMMTAAREALVPFGKD
APLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQKCGLDGVVCSAQEAVRFKQVF
GQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYMVIGRPVTQSVDPAQTLKAINAS
LQRSA
References
External Links:
ResourceLink
Uniprot ID:P08244
Uniprot Name:PYRF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062598
PDB ID:1EIX
Ecogene ID:EG10809
Ecocyc:EG10809
ColiBase:b1281
Kegg Gene:b1281
EchoBASE ID:EB0802
CCDB:PYRF_ECOLI
BacMap:16129242
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Harris, P., Navarro Poulsen, J. C., Jensen, K. F., Larsen, S. (2000). "Structural basis for the catalytic mechanism of a proficient enzyme: orotidine 5'-monophosphate decarboxylase." Biochemistry 39:4217-4224. Pubmed: 10757968
  • Harris, P., Poulsen, J. C., Jensen, K. F., Larsen, S. (2002). "Substrate binding induces domain movements in orotidine 5'-monophosphate decarboxylase." J Mol Biol 318:1019-1029. Pubmed: 12054799
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hidaka, M., Akiyama, M., Horiuchi, T. (1988). "A consensus sequence of three DNA replication terminus sites on the E. coli chromosome is highly homologous to the terR sites of the R6K plasmid." Cell 55:467-475. Pubmed: 3052852
  • Poulsen, J. C., Harris, P., Jensen, K. F., Larsen, S. (2001). "Selenomethionine substitution of orotidine-5'-monophosphate decarboxylase causes a change in crystal contacts and space group." Acta Crystallogr D Biol Crystallogr 57:1251-1259. Pubmed: 11526316
  • Turnbough, C. L. Jr, Kerr, K. H., Funderburg, W. R., Donahue, J. P., Powell, F. E. (1987). "Nucleotide sequence and characterization of the pyrF operon of Escherichia coli K12." J Biol Chem 262:10239-10245. Pubmed: 2956254