D-alanine--D-alanine ligase B (P07862)

Identification
Name:D-alanine--D-alanine ligase B
Synonyms:
  • D-Ala-D-Ala ligase B
  • D-alanylalanine synthetase B
Gene Name:ddlB
Enzyme Class:
Biological Properties
General Function:Involved in ATP binding
Specific Function:Cell wall formation
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
2.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
2.0D-Alanine + 1.0Adenosine triphosphate ↔ 1.0ADP + 1.0D-Alanyl-D-alanine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+2.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 2.0D-Alanine ↔ 1.0ADP + 1.0Phosphate + 1.0D-Alanyl-D-alanine
ReactionCard
SMPDB Reactions:
2.0Thumb+1.0Thumb → 1.0Thumb+1.0Adenosine diphosphate+1.0Thumb+1.0Thumb
2.0D-Alanine + 1.0Adenosine triphosphate → 1.0D-Alanyl-D-alanine + 1.0Adenosine diphosphate + 1.0Phosphate + 1.0ADP
ReactionCard
EcoCyc Reactions:
2.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
2.0D-Alanine + 1.0Adenosine triphosphate ↔ 1.0ADP + 1.0D-Alanyl-D-alanine + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB01310D-AlanineMetaboCard
ECMDB03459D-Alanyl-D-alanineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Component
cell part
cell wall
cytoplasm
external encapsulating structure
intracellular part
Function
acid-amino acid ligase activity
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
D-alanine-D-alanine ligase activity
ligase activity
ligase activity, forming carbon-nitrogen bonds
nucleoside binding
purine nucleoside binding
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b0092
Gene OrientationClockwise
Centisome Percentage:2.20
Left Sequence End102233
Right Sequence End103153
Gene Sequence:
>921 bp
ATGACTGATAAAATCGCGGTCCTGTTGGGTGGGACCTCCGCTGAGCGGGAAGTTTCTCTG
AATTCTGGCGCAGCGGTGTTAGCCGGACTGCGTGAAGGCGGTATTGACGCGTATCCTGTC
GACCCGAAAGAAGTCGACGTGACGCAACTGAAGTCGATGGGCTTTCAGAAAGTGTTTATC
GCGCTACACGGTCGCGGCGGTGAAGATGGTACGCTGCAGGGGATGCTCGAGCTGATGGGC
TTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGC
AAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCA
GAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCG
GTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCAAAAGTAGTAGCAGAA
AATGCTCTACAAGATGCATTAAGATTGGCATTTCAGCACGATGAAGAAGTATTGATTGAA
AAATGGCTAAGTGGGCCGGAGTTCACGGTTGCGATACTCGGTGAAGAAATTTTACCGTCA
ATACGTATTCAACCGTCCGGAACCTTCTATGATTATGAGGCGAAGTATCTCTCTGATGAG
ACACAGTATTTCTGCCCCGCAGGTCTGGAAGCGTCACAAGAGGCCAATTTGCAGGCATTA
GTGCTGAAAGCATGGACGACGTTAGGTTGCAAAGGATGGGGACGTATTGACGTTATGCTG
GACAGCGATGGACAGTTTTATCTGCTGGAAGCCAATACCTCACCGGGTATGACCAGCCAC
AGCCTGGTGCCGATGGCGGCACGTCAGGCAGGTATGAGCTTCTCGCAGTTGGTAGTACGA
ATTCTGGAACTGGCGGACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:306
Protein Molecular Weight:32839
Protein Theoretical pI:4
PDB File:1IOV
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>D-alanine--D-alanine ligase B
MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKEVDVTQLKSMGFQKVFI
ALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKLLWQGAGLPVAPWVALTRA
EFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAENALQDALRLAFQHDEEVLIE
KWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLSDETQYFCPAGLEASQEANLQAL
VLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPGMTSHSLVPMAARQAGMSFSQLVVR
ILELAD
References
External Links:
ResourceLink
Uniprot ID:P07862
Uniprot Name:DDLB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321973
PDB ID:1IOV
Ecogene ID:EG10214
Ecocyc:EG10214
ColiBase:b0092
Kegg Gene:b0092
EchoBASE ID:EB0210
CCDB:DDLB_ECOLI
BacMap:16128085
General Reference:
  • al-Bar, O. A., O'Connor, C. D., Giles, I. G., Akhtar, M. (1992). "D-alanine: D-alanine ligase of Escherichia coli. Expression, purification and inhibitory studies on the cloned enzyme." Biochem J 282 ( Pt 3):747-752. Pubmed: 1554356
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Dewar, S. J., Donachie, W. D. (1990). "Regulation of expression of the ftsA cell division gene by sequences in upstream genes." J Bacteriol 172:6611-6614. Pubmed: 2228979
  • Fan, C., Moews, P. C., Walsh, C. T., Knox, J. R. (1994). "Vancomycin resistance: structure of D-alanine:D-alanine ligase at 2.3 A resolution." Science 266:439-443. Pubmed: 7939684
  • Fan, C., Park, I. S., Walsh, C. T., Knox, J. R. (1997). "D-alanine:D-alanine ligase: phosphonate and phosphinate intermediates with wild type and the Y216F mutant." Biochemistry 36:2531-2538. Pubmed: 9054558
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ikeda, M., Wachi, M., Jung, H. K., Ishino, F., Matsuhashi, M. (1990). "Nucleotide sequence involving murG and murC in the mra gene cluster region of Escherichia coli." Nucleic Acids Res 18:4014. Pubmed: 2197603
  • Robinson, A. C., Kenan, D. J., Sweeney, J., Donachie, W. D. (1986). "Further evidence for overlapping transcriptional units in an Escherichia coli cell envelope-cell division gene cluster: DNA sequence and transcriptional organization of the ddl ftsQ region." J Bacteriol 167:809-817. Pubmed: 3528126
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901