Phosphate transport system permease protein pstA (P07654)

Identification
Name:Phosphate transport system permease protein pstA
Synonyms:Not Available
Gene Name:pstA
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Transports:
Transport References:
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+2.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0Phosphate → 1.0ADP + 1.0Hydrogen ion + 2.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
membrane
Function
anion transmembrane transporter activity
inorganic anion transmembrane transporter activity
inorganic phosphate transmembrane transporter activity
ion transmembrane transporter activity
phosphate transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
phosphate transport
transport
Gene Properties
Blattner:b3726
Gene OrientationCounterclockwise
Centisome Percentage:84.20
Left Sequence End3906572
Right Sequence End3907462
Gene Sequence:
>891 bp
ATGAGCGGCTTACCTCTTATTTCGCGCCGTCGACTGTTAACGGCGATGGCGCTTTCTCCG
TTGTTATGGCAGATGAATACCGCCCACGCGGCGGCTATTGATCCCAATCGTATTGTGGCG
CTGGAGTGGTTGCCGGTGGAATTACTGCTGGCGCTCGGCATCGTGCCTTACGGCGTGGCG
GATACCATCAACTATCGCCTGTGGGTCAGCGAACCACCATTGCCGGACTCAGTGATCGAC
GTCGGTTTGCGCACAGAACCTAACCTTGAACTGCTGACCGAAATGAAACCATCGTTTATG
GTCTGGTCGGCAGGATATGGCCCTTCACCAGAAATGCTGGCTCGTATTGCGCCGGGTCGC
GGATTTAACTTCAGTGACGGCAAACAGCCGTTGGCGATGGCGCGTAAATCGCTGACGGAA
ATGGCAGATTTACTTAACCTGCAAAGCGCAGCGGAAACGCATTTAGCGCAATATGAAGAC
TTTATCCGCAGCATGAAACCCCGCTTTGTGAAGCGTGGTGCGCGTCCGTTATTGCTGACG
ACGCTTATCGATCCGCGCCATATGCTGGTCTTCGGTCCAAACAGCTTGTTCCAGGAAATT
CTTGATGAGTACGGCATCCCAAATGCCTGGCAAGGGGAAACCAACTTCTGGGGCAGTACC
GCCGTCAGTATCGATCGTCTGGCGGCGTATAAAGACGTTGATGTGCTCTGTTTTGATCAC
GACAACAGCAAAGACATGGATGCGCTAATGGCAACGCCGCTGTGGCAGGCCATGCCGTTT
GTCCGCGCCGGACGCTTTCAGCGCGTACCTGCAGTCTGGTTTTATGGTGCGACGCTCTCG
GCAATGCACTTTGTGCGCGTTCTGGATAACGCCATCGGAGGTAAAGCGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:296
Protein Molecular Weight:32321
Protein Theoretical pI:11
Signaling Regions:
  • None
Transmembrane Regions:
  • 29-50
  • 83-102
  • 127-146
  • 151-169
  • 205-223
  • 267-286
Protein Sequence:
>Phosphate transport system permease protein pstA
MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFWLIWILMSTITRGIDGM
SLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGIMAGIYLAEYGRKSWLAEV
IRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIALALLQVPIVIRTTENMLKLVP
YSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAIARIAGETAPLLFTALSNQFWST
DMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIITLCVLLLNILARVVFAKNKHG
References
External Links:
ResourceLink
Uniprot ID:P07654
Uniprot Name:PSTA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674358
Ecogene ID:EG10782
Ecocyc:EG10782
ColiBase:b3726
Kegg Gene:b3726
EchoBASE ID:EB0775
CCDB:PSTA_ECOLI
BacMap:16131594
General Reference:
  • Amemura, M., Makino, K., Shinagawa, H., Kobayashi, A., Nakata, A. (1985). "Nucleotide sequence of the genes involved in phosphate transport and regulation of the phosphate regulon in Escherichia coli." J Mol Biol 184:241-250. Pubmed: 2993631
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Drew, D., Sjostrand, D., Nilsson, J., Urbig, T., Chin, C. N., de Gier, J. W., von Heijne, G. (2002). "Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis." Proc Natl Acad Sci U S A 99:2690-2695. Pubmed: 11867724
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Surin, B. P., Rosenberg, H., Cox, G. B. (1985). "Phosphate-specific transport system of Escherichia coli: nucleotide sequence and gene-polypeptide relationships." J Bacteriol 161:189-198. Pubmed: 3881386
  • Webb, D. C., Rosenberg, H., Cox, G. B. (1992). "Mutational analysis of the Escherichia coli phosphate-specific transport system, a member of the traffic ATPase (or ABC) family of membrane transporters. A role for proline residues in transmembrane helices." J Biol Chem 267:24661-24668. Pubmed: 1447208