Ornithine carbamoyltransferase chain F (P06960)

Identification
Name:Ornithine carbamoyltransferase chain F
Synonyms:
  • OTCase-2
Gene Name:argF
Enzyme Class:
Biological Properties
General Function:Involved in carboxyl- or carbamoyltransferase activity
Specific Function:Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Carbamoylphosphate + 1.0Ornithine + 1.0L-Ornithine ↔ 1.0Citrulline + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Carbamoylphosphate + 1.0Ornithine ↔ 1.0Phosphate + 1.0Citrulline
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Ornithine + 1.0Carbamoylphosphate ↔ 1.0Hydrogen ion + 1.0Citrulline + 1.0Phosphate
ReactionCard
SMPDB Reactions:
1.0Ornithine+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Ornithine + 1.0Carbamoylphosphate + 1.0Ornithine → 1.0Phosphate + 1.0Hydrogen ion + 1.0Citrulline
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Carbamoylphosphate + 1.0Ornithine + 1.0L-Ornithine ↔ 1.0Citrulline + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Ornithine + 1.0Carbamoylphosphate ↔ 1.0Hydrogen ion + 1.0Citrulline + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01096CarbamoylphosphateMetaboCard
ECMDB00904CitrullineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB23898L-OrnithineMetaboCard
ECMDB00214OrnithineMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Component
macromolecular complex
ornithine carbamoyltransferase complex
protein complex
Function
amino acid binding
binding
carboxyl- or carbamoyltransferase activity
carboxylic acid binding
catalytic activity
ornithine carbamoyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b0273
Gene OrientationCounterclockwise
Centisome Percentage:6.22
Left Sequence End288525
Right Sequence End289529
Gene Sequence:
>1005 bp
GTGAAACTGGATGAAATCGCTCGGCTGGCGGGAGTGTCGCGGACCACTGCAAGCTATGTT
ATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCT
GTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGC
ACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCT
AACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAA
GATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCC
ATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGAC
CCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTT
GGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAG
ACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGT
TTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTAT
GAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAG
GCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGC
GACGGCAAACTGCCTTCTGACCTGGCAATTGCCACCTTTGGCGATAACGAACTGCTCGAC
TTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTG
CTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGC
ATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:334
Protein Molecular Weight:36827
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Ornithine carbamoyltransferase chain F
MSDLYKKHFLKLLDFTPAQFTSLLTLAAQLKADKKNGKEVQKLTGKNIALIFEKDSTRTR
CSFEVAAFDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGHGQEVVETLAQY
AGVPVWNGLTNEFHPTQLLADLMTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALT
GLDLRLLAPKACWPEESLVAECSALAEKHGGKITLTEDVAAGVKGADFIYTDVWVSMGEA
KEKWAERIALLRGYQVNAQMMALTDNPNVKFLHCLPAFHDDQTTLGKQMAKEFDLHGGME
VTDEVFESAASIVFDQAENRMHTIKAVMMATLGE
References
External Links:
ResourceLink
Uniprot ID:P06960
Uniprot Name:OTC2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321961
Ecogene ID:EG10067
Ecocyc:EG10067
ColiBase:b0273
Kegg Gene:b0273
EchoBASE ID:EB0065
CCDB:OTC2_ECOLI
BacMap:16128258
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Falmagne, P., Portetelle, D., Stalon, V. (1985). "Immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria." J Bacteriol 161:714-719. Pubmed: 3968036
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Van Vliet, F., Cunin, R., Jacobs, A., Piette, J., Gigot, D., Lauwereys, M., Pierard, A., Glansdorff, N. (1984). "Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferases--complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB." Nucleic Acids Res 12:6277-6289. Pubmed: 6382166