ECMDB

Identification

Name:

Ornithine carbamoyltransferase chain F

Synonyms:

OTCase-2

Gene Name:

argF

Enzyme Class:

2.1.3.3

Biological Properties

General Function:

Involved in carboxyl- or carbamoyltransferase activity

Specific Function:

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline

Cellular Location:

Cytoplasm

SMPDB Pathways:

arginine metabolism PW000790

KEGG Pathways:

Arginine and proline metabolism ec00330
Metabolic pathways eco01100

KEGG Reactions:

1.0

↔

1.0

1.0Carbamoylphosphate + 1.0Ornithine + 1.0L-Ornithine ↔ 1.0Citrulline + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.0Carbamoylphosphate + 1.0Ornithine ↔ 1.0Phosphate + 1.0Citrulline
ReactionCard

1.0

↔

1.0

1.0Ornithine + 1.0Carbamoylphosphate ↔ 1.0Hydrogen ion + 1.0Citrulline + 1.0Phosphate
ReactionCard

SMPDB Reactions:

1.0Ornithine

1.0

→

1.0

1.0Ornithine + 1.0Carbamoylphosphate + 1.0Ornithine → 1.0Phosphate + 1.0Hydrogen ion + 1.0Citrulline
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Carbamoylphosphate + 1.0Ornithine + 1.0L-Ornithine ↔ 1.0Citrulline + 1.0Hydrogen ion + 1.0Phosphate
ReactionCard

1.0

↔

1.0

1.0Ornithine + 1.0Carbamoylphosphate ↔ 1.0Hydrogen ion + 1.0Citrulline + 1.0Phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01096	Carbamoylphosphate	MetaboCard
ECMDB00904	Citrulline	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB21380	Inorganic phosphate	MetaboCard
ECMDB23898	L-Ornithine	MetaboCard
ECMDB00214	Ornithine	MetaboCard
ECMDB01429	Phosphate	MetaboCard

GO Classification:

Component
macromolecular complex
ornithine carbamoyltransferase complex
protein complex
Function
amino acid binding
binding
carboxyl- or carbamoyltransferase activity
carboxylic acid binding
catalytic activity
ornithine carbamoyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process

Gene Properties

Blattner:

b0273

Gene Orientation

Counterclockwise

Centisome Percentage:

6.22

Left Sequence End

288525

Right Sequence End

289529

Gene Sequence:

>1005 bp
GTGAAACTGGATGAAATCGCTCGGCTGGCGGGAGTGTCGCGGACCACTGCAAGCTATGTT
ATTAACGGCAAAGCGAAGCAATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCT
GTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAGCTGGGCTTCGTGCTGGACGC
ACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCT
AACTATCTTGAACGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAA
GATCAGCCAGACAACGAAATGCGGTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCC
ATTATTGTTTCGACGTCGTTGCCTCCTGAGCATCCTTTTTATCAACGCTGGGCTAACGAC
CCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACACTTCACCAGCGTGGTT
GGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAG
ACGGTGCTTTATCTTGGTGCGCTACCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGT
TTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATTTCCTGTATGCCAACAGCTAT
GAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAG
GCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGAGTGATGGATGTCACGCTGCGTCGC
GACGGCAAACTGCCTTCTGACCTGGCAATTGCCACCTTTGGCGATAACGAACTGCTCGAC
TTCTTACAGTGTCCGGTGCTGGCAGTGGCTCAACGTCACCGCGATGTCGCAGAGCGTGTG
CTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGC
ATTAAACGTAATCTCTATCGCCGCGGCGTGCTCAGCCGTAGCTAA

Protein Properties

Pfam Domain Function:

OTCace (PF00185 )
OTCace_N (PF02729 )

Protein Residues:

334

Protein Molecular Weight:

36827

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Ornithine carbamoyltransferase chain F
MSDLYKKHFLKLLDFTPAQFTSLLTLAAQLKADKKNGKEVQKLTGKNIALIFEKDSTRTR
CSFEVAAFDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYDGIQYRGHGQEVVETLAQY
AGVPVWNGLTNEFHPTQLLADLMTMQEHLPGKAFNEMTLVYAGDARNNMGNSMLEAAALT
GLDLRLLAPKACWPEESLVAECSALAEKHGGKITLTEDVAAGVKGADFIYTDVWVSMGEA
KEKWAERIALLRGYQVNAQMMALTDNPNVKFLHCLPAFHDDQTTLGKQMAKEFDLHGGME
VTDEVFESAASIVFDQAENRMHTIKAVMMATLGE

References

External Links:

Resource	Link
Uniprot ID:	P06960
Uniprot Name:	OTC2_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	21321961
Ecogene ID:	EG10067
Ecocyc:	EG10067
ColiBase:	b0273
Kegg Gene:	b0273
EchoBASE ID:	EB0065
CCDB:	OTC2_ECOLI
BacMap:	16128258

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Falmagne, P., Portetelle, D., Stalon, V. (1985). "Immunological and structural relatedness of catabolic ornithine carbamoyltransferases and the anabolic enzymes of enterobacteria." J Bacteriol 161:714-719. Pubmed: 3968036
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Van Vliet, F., Cunin, R., Jacobs, A., Piette, J., Gigot, D., Lauwereys, M., Pierard, A., Glansdorff, N. (1984). "Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferases--complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB." Nucleic Acids Res 12:6277-6289. Pubmed: 6382166

Ornithine carbamoyltransferase chain F (P06960)