Evolved beta-galactosidase subunit alpha (P06864)

Identification
Name:Evolved beta-galactosidase subunit alpha
Synonyms:
  • Beta-gal
  • Lactase
Gene Name:ebgA
Enzyme Class:
Biological Properties
General Function:Involved in beta-galactosidase activity
Specific Function:The wild-type enzyme is an ineffective lactase. Two classes of point mutations dramatically improve activity of the enzyme
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0alpha-Lactose + 1.0Water ↔ 1.0alpha-D-Glucose + 1.0D-Galactose
ReactionCard
1.0Lactose+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Lactose + 1.0Water ↔ 1.0D-Glucose + 1.0D-Galactose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03345alpha-D-GlucoseMetaboCard
ECMDB00186alpha-LactoseMetaboCard
ECMDB00143D-GalactoseMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
beta-galactosidase complex
macromolecular complex
protein complex
Function
beta-galactosidase activity
binding
carbohydrate binding
catalytic activity
cation binding
galactosidase activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
ion binding
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3076
Gene OrientationClockwise
Centisome Percentage:69.42
Left Sequence End3220655
Right Sequence End3223747
Gene Sequence:
>3093 bp
ATGAATCGCTGGGAAAACATTCAGCTCACCCACGAAAACCGACTTGCGCCGCGTGCGTAC
TTTTTTTCATATGATTCTGTTGCGCAAGCGCGTACCTTTGCCCGCGAAACCAGCAGCCTG
TTTCTGCCCTTAAGCGGTCAGTGGAATTTCCACTTTTTTGACCATCCGCTGCAAGTACCA
GAAGCCTTCACCTCTGAGTTAATGGCTGACTGGGGGCATATTACCGTCCCCGCCATGTGG
CAAATGGAAGGTCACGGCAAACTGCAATATACCGACGAAGGTTTTCCGTTCCCCATCGAT
GTGCCGTTTGTCCCCAGCGATAACCCAACCGGTGCCTATCAACGTATTTTCACCCTCAGC
GACGGCTGGCAGGGTAAACAGACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAA
GTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTT
GACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGG
GCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGAT
GTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGTGCGTACCGACTTT
GACGAAGCCTATTGCGATGCCACGCTTTCCTGCGAAGTGGTGCTGGAAAATCTCGCCGCC
TCCCCTGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGC
AGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTC
GAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTG
AAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATC
AAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAAC
CGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAT
CTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGAT
CCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTC
GAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGG
GAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCG
ATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTAC
CATGCGGCGAAAGCGCTGGATGACACGCGACTGGTGCATTACGAAGAAGATCGCGATGCT
GAAGTGGTCGATATTATTTCCACCATGTACACCCGCGTGCCGCTGATGAATGAGTTTGGT
GAATACCCGCATCCGAAGCCGCGCATCATCTGTGAATATGCTCATGCGATGGGGAACGGA
CCGGGCGGGCTGACGGAGTACCAGAACGTCTTCTATAAGCACGATTGCATTCAGGGTCAT
TATGTCTGGGAGTGGTGCGACCACGGGATCCAGGCACAGGACGACCACGGCAATGTCTGG
TATAAATTCGGCGGCGACTACGGCGACTATCCCAACAACTATAACTTCTGTCTTGATGGT
TTGATCTATTCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCG
CCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTG
TGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACG
CTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAG
ATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGAT
TCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAG
GAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAA
GACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATG
AGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAG
ATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAA
CCGAATCATTTGCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGAT
GGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATG
CGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCGGC
GAGCGTTACGGCGACTATCCGCACATCATTCCGTGCATCGGTTTCACCATGGGAATTAAC
GGCGAATACGATCAGGTGGCGTATTACGGTCGTGGACCGGGCGAAAACTACGCCGACAGC
CAGCAGGCTAACATCATCGATATCTGGCGCAGCACCGTCGATGCCATGTTCGAGAACTAT
CCCTTCCCGCAGAACAACGGTAACCGTCAGCATGTCCGCTGGACGGCACTGACTAACCGC
CACGGTAACGGTCTGCTGGTGGTTCCGCAGCGCCCAATTAACTTCAGCGCCTGGCACTAT
ACCCAGGAAAACATCCACGCTGCCCAGCACTGTAACGAGCTGCAGCGCAGTGATGACATC
ACCCTGAACCTCGATCACCAGCTGCTTGGCCTCGGCTCCAACTCCTGGGGCAGCGAGGTG
CTGGACTCCTGGCGCGTCTGGTTCCGTGACTTCAGCTACGGCTTTACGTTGCTGCCGGTT
TCTGGCGGAGAAGCTACCGCGCAAAGCCTGGCGTCGTATGAGTTCGGCGCAGGGTTCTTT
TCCACGAATTTGCACAGCGAGAATAAGCAATGA
Protein Properties
Pfam Domain Function:
Protein Residues:1030
Protein Molecular Weight:117878
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Evolved beta-galactosidase subunit alpha
MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPLSGQWNFHFFDHPLQVP
EAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVPFVPSDNPTGAYQRIFTLS
DGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEFDISAMVKTGDNLLCVRVMQW
ADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRTDFDEAYCDATLSCEVVLENLAA
SPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASFAFTVEQPQQWSAESPYLYHLVMTL
KDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYVMLHGVNRHDNDHRKGRAVGMDRVEKD
LQLMKQHNINSVRTAHYPNDPRFYELCDIYGLFVMAETDVESHGFANVGDISRITDDPQW
EKVYVERIVRHIHAQKNHPSIIIWSLGNESGYGCNIRAMYHAAKALDDTRLVHYEEDRDA
EVVDIISTMYTRVPLMNEFGEYPHPKPRIICEYAHAMGNGPGGLTEYQNVFYKHDCIQGH
YVWEWCDHGIQAQDDHGNVWYKFGGDYGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIA
PVKIHARDLTRGELKVENKLWFTTLDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQ
ITLPQLDAREAFLNITVTKDSRTRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLE
DDRLSCTVRGYNFAITFSKMSGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQ
PNHLQIMQEHLRDFAVEQSDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSG
ERYGDYPHIIPCIGFTMGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENY
PFPQNNGNRQHVRWTALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDI
TLNLDHQLLGLGSNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFF
STNLHSENKQ
References
External Links:
ResourceLink
Uniprot ID:P06864
Uniprot Name:BGA2_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675876
Ecogene ID:EG10252
Ecocyc:EG10252
ColiBase:b3076
Kegg Gene:b3076
EchoBASE ID:EB0248
CCDB:BGA2_ECOLI
BacMap:49176308
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Elliott, A. C., K, S., Sinnott, M. L., Smith, P. J., Bommuswamy, J., Guo, Z., Hall, B. G., Zhang, Y. (1992). "The catalytic consequences of experimental evolution. Studies on the subunit structure of the second (ebg) beta-galactosidase of Escherichia coli, and on catalysis by ebgab, an experimental evolvant containing two amino acid substitutions." Biochem J 282 ( Pt 1):155-164. Pubmed: 1540130
  • Fowler, A. V., Smith, P. J. (1983). "The active site regions of lacZ and ebg beta-galactosidases are homologous." J Biol Chem 258:10204-10207. Pubmed: 6411710
  • Hall, B. G., Betts, P. W., Wootton, J. C. (1989). "DNA sequence analysis of artificially evolved ebg enzyme and ebg repressor genes." Genetics 123:635-648. Pubmed: 2515108
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293
  • Stokes, H. W., Betts, P. W., Hall, B. G. (1985). "Sequence of the ebgA gene of Escherichia coli: comparison with the lacZ gene." Mol Biol Evol 2:469-477. Pubmed: 3939707