Alpha-galactosidase (P06720)

Identification
Name:Alpha-galactosidase
Synonyms:
  • Melibiase
Gene Name:melA
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity, hydrolyzing O-glycosyl compounds
Specific Function:Hydrolysis of terminal, non-reducing alpha-D- galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Water + 1.0Melibiose → 1.0D-Galactose + 1.0D-Glucose
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Melibiose + 1.0Water ↔ 1.0D-Galactose + 1.0D-Glucose
ReactionCard
1.0Digalactosylceramide+1.0Thumb ↔ 1.0Galactosylceramide+1.0Thumb
1.0Digalactosylceramide + 1.0Water ↔ 1.0Galactosylceramide + 1.0D-Galactose
ReactionCard
1.0Digalactosyl-diacylglycerol+1.0Thumb ↔ 1.01,2-Diacyl-3-beta-D-galactosyl-sn-glycerol+1.0Thumb
1.0Digalactosyl-diacylglycerol + 1.0Water ↔ 1.01,2-Diacyl-3-beta-D-galactosyl-sn-glycerol + 1.0D-Galactose
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Gal alpha 1->6D-Gal alpha 1->6D-Glucose + 1.0Water ↔ 1.0D-Galactose + 1.0Melibiose
ReactionCard
1.0Thumb+1.0Globotriaosylceramide ↔ 1.0Thumb+1.0Lactosylceramide
1.0Water + 1.0Globotriaosylceramide ↔ 1.0D-Galactose + 1.0Lactosylceramide
ReactionCard
1.0Melibiose+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Melibiose + 1.0Water ↔ 1.0D-Galactose + 1.0D-Glucose
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Beta-D-Glucose+1.0Thumb
1.0Melibiose + 1.0Water → 1.0Alpha-D-Galactose + 1.0Beta-D-Glucose + 1.0b-D-Glucose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04012Alpha-D-GalactoseMetaboCard
ECMDB00516b-D-GlucoseMetaboCard
ECMDB20139D-Gal alpha 1->6D-Gal alpha 1->6D-GlucoseMetaboCard
ECMDB00143D-GalactoseMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB00169D-MannoseMetaboCard
ECMDB00131GlycerolMetaboCard
ECMDB00160InositolMetaboCard
ECMDB00048MelibioseMetaboCard
ECMDB00247SorbitolMetaboCard
ECMDB00258SucroseMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b4119
Gene OrientationClockwise
Centisome Percentage:93.54
Left Sequence End4339934
Right Sequence End4341289
Gene Sequence:
>1356 bp
ATGTTGTCAAAATTTAAGCGTAATAAACATCAACAACACCTTGCCCAACTACCCAAGATT
TCTCAATCAGTTGATGATGTCGATTTCTTTTACGCTCCCGCCGACTTCCGGGAGACGCTG
CTGGAAAAAATAGCCAGCGCGAAGCAGCGCATTTGCATTGTCGCCCTGTATCTCGAACAG
GATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAA
CTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCG
GCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGAT
GTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAGCCCTTGGTGTTCTGCACTTTAAA
GGCTTTATCATCGACGATAGCGTACTTTATAGCGGTGCCAGCCTGAACGATGTTTACCTG
CATCAGCACGATAAATATCGCTACGACCGTTATCATCTGATCCGTAACCGTAAGATGTCA
GACATTATGTTTGAATGGGTTACACAGAATATTATGAATGGCCGCGGCGTTAATCGTCTG
GATGATGTTAATCGGCCAAAAAGCCCGGAAATCAAGAACGATATTCGTCTGTTCCGCCAG
GAGCTGCGTGATGCCGCTTATCATTTCCAGGGCGATGCCGACAACGATCAGCTTTCTGTA
ACGCCGCTAGTGGGGCTGGGGAAATCGAGTCTGTTGAACAAGACCATTTTCCATCTTATG
CCTTGTGCCGAGCAGAAACTAACCATCTGTACGCCATACTTCAACCTGCCAGCAATCCTT
GTGCGCAATATTATCCAGTTGCTGCGCGAAGGGAAAAAGGTCGAAATTATTGTTGGTGAT
AAAACCGCGAATGACTTCTACATTCCGGAAGATGAACCTTTCAAGATAATTGGCGCATTG
CCTTATCTCTATGAGATCAATCTGCGTCGTTTCCTGAGCCGTTTGCAGTATTACGTCAAT
ACTGACCAGCTAGTGGTTCGGTTATGGAAAGATGACGACAACACCTATCACCTGAAAGGG
ATGTGGGTTGATGATAAGTGGATGTTGATCACCGGTAATAACCTGAACCCGCGCGCCTGG
CGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAG
CGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGAT
CTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGT
ATCCGCATCGACCGATTAATTAGCCGCATCCTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:451
Protein Molecular Weight:50657
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Alpha-galactosidase
MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPTRLEESHIVVRKLMDSA
GASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVCKRHGLEQTIADTLGPGGI
MRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAMYARYPHIKQVGLCHSVQGTA
EELARDLNIDPATLRYRCAGINHMAFYLELERKTADGSYVNLYPELLAAYEAGQAPKPNI
HGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWFIKPGREDLIERYKVPLDEYPKRCV
EQLANWHKELEEYKKASRIDIKPSREYASTIMNAIWTGEPSVIYGNVRNDGLIDNLPQGC
CVEVACLVDANGIQPTKVGTLPSHLAALMQTNINVQTLLTEAILTENRDRVYHAAMMDPH
TAAVLGIDEIYALVDDLIAAHGDWLPGWLHR
References
External Links:
ResourceLink
Uniprot ID:P06720
Uniprot Name:AGAL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675473
Ecogene ID:EG10577
Ecocyc:EG10577
ColiBase:b4119
Kegg Gene:b4119
EchoBASE ID:EB0572
CCDB:AGAL_ECOLI
BacMap:16131945
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Liljestrom, P. L., Liljestrom, P. (1987). "Nucleotide sequence of the melA gene, coding for alpha-galactosidase in Escherichia coli K-12." Nucleic Acids Res 15:2213-2220. Pubmed: 3031590
  • Nagao, Y., Nakada, T., Imoto, M., Shimamoto, T., Sakai, S., Tsuda, M., Tsuchiya, T. (1988). "Purification and analysis of the structure of alpha-galactosidase from Escherichia coli." Biochem Biophys Res Commun 151:236-241. Pubmed: 2831880
  • Shimamoto, T., Yazyu, H., Futai, M., Tsuchiya, T. (1984). "Nucleotide sequence of the promoter region of the melibiose operon of Escherichia coli." Biochem Biophys Res Commun 121:41-46. Pubmed: 6329200