Vitamin B12 transport periplasmic protein BtuE (P06610)

Identification
Name:Vitamin B12 transport periplasmic protein BtuE
Synonyms:Not Available
Gene Name:btuE
Enzyme Class:
Biological Properties
General Function:Involved in glutathione peroxidase activity
Specific Function:Not essential for B12 transport; however, it is an auxiliary component of the transport system
Cellular Location:Periplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
2.0Thumb+1.0Thumb ↔ 1.0Thumb+2.0Thumb
2.0Glutathione + 1.0Hydrogen peroxide ↔ 1.0Glutathione disulfide + 2.0Water
ReactionCard
EcoCyc Reactions:
2.0Thumb+1.0Thumb ↔ 1.0Thumb+2.0Thumb
2.0Glutathione + 1.0Hydrogen peroxide ↔ 1.0Glutathione disulfide + 2.0Water
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen peroxide + 1.0Glutathione → 1.0Glutathione disulfide + 1.0Water
ReactionCard
Complex Reactions:
1.0Thumb+1.0Reduced Thioredoxin → 2.0Thumb+1.0Oxidized Thioredoxin
1.0Hydrogen peroxide + 1.0Reduced Thioredoxin → 2.0Water + 1.0Oxidized Thioredoxin
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB011935(S)-Hydroperoxyeicosatetraenoic acidMetaboCard
ECMDB00125GlutathioneMetaboCard
ECMDB21221Glutathione disulfideMetaboCard
ECMDB21232Hydrogen peroxideMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
antioxidant activity
glutathione peroxidase activity
peroxidase activity
Process
metabolic process
oxidation reduction
response to oxidative stress
response to stimulus
response to stress
Gene Properties
Blattner:b1710
Gene OrientationCounterclockwise
Centisome Percentage:38.61
Left Sequence End1791582
Right Sequence End1792133
Gene Sequence:
>552 bp
ATGTTGTTAGAACAGGGGTGGCTGGTTGGCGCGCGCCGCGTTCCCTCACCACATTACGAT
TGCCGCCCGGATGACGAAACACCCACCCTGCTGGTGGTGCACAATATTAGCCTGCCGCCA
GGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTGGAACTATTGATCCGCAGGCA
CATCCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGT
GATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCT
CAGTATCAGGGGCGCGAACGCTGCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACC
GATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTG
ATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGAT
CGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAG
GAGACAACATGA
Protein Properties
Pfam Domain Function:
Protein Residues:183
Protein Molecular Weight:20469
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Vitamin B12 transport periplasmic protein BtuE
MQDSILTTVVKDIDGEVTTLEKFAGNVLLIVNVASKCGLTPQYEQLENIQKAWVDRGFMV
LGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGEGRHPLYQKLIAAAPTAVA
PEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQRFSPDMTPEDPIVMESIKLA
LAK
References
External Links:
ResourceLink
Uniprot ID:P06610
Uniprot Name:BTUE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21238958
Ecogene ID:EG10129
Ecocyc:EG10129
ColiBase:b1710
Kegg Gene:b1710
EchoBASE ID:EB0127
CCDB:BTUE_ECOLI
BacMap:16129666
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Friedrich, M. J., de Veaux, L. C., Kadner, R. J. (1986). "Nucleotide sequence of the btuCED genes involved in vitamin B12 transport in Escherichia coli and homology with components of periplasmic-binding-protein-dependent transport systems." J Bacteriol 167:928-934. Pubmed: 3528129
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553