Dihydroorotase (P05020)

Identification
Name:Dihydroorotase
Synonyms:
  • DHOase
Gene Name:pyrC
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity
Specific Function:(S)-dihydroorotate + H(2)O = N-carbamoyl-L- aspartate
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.04,5-Dihydroorotic acid + 1.0Water ↔ 1.0Ureidosuccinic acid + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.04,5-Dihydroorotic acid + 1.0Water ↔ 1.0Ureidosuccinic acid
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.04,5-Dihydroorotic acid+1.0Thumb
1.0N-carbamoyl-L-aspartate + 1.0Hydrogen ion → 1.0Water + 1.04,5-Dihydroorotic acid + 1.04,5-Dihydroorotic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.04,5-Dihydroorotic acid + 1.0Water ↔ 1.0Ureidosuccinic acid + 1.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB005284,5-Dihydroorotic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB24189N-carbamoyl-L-aspartateMetaboCard
ECMDB00828Ureidosuccinic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
dihydroorotase activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
Process
cellular aromatic compound metabolic process
cellular metabolic process
metabolic process
nucleobase metabolic process
pyrimidine base biosynthetic process
pyrimidine base metabolic process
Gene Properties
Blattner:b1062
Gene OrientationCounterclockwise
Centisome Percentage:24.16
Left Sequence End1120784
Right Sequence End1121830
Gene Sequence:
>1047 bp
ATGACTGCACCATCCCAGGTATTAAAGATCCGCCGCCCAGACGACTGGCACCTTCACCTC
CGCGATGGCGACATGTTAAAAACTGTCGTGCCATATACCAGCGAAATTTATGGACGGGCT
ATCGTAATGCCCAATCTGGCTCCGCCCGTGACCACCGTTGAGGCTGCCGTGGCGTATCGC
CAGCGTATTCTTGACGCCGTACCTGCCGGGCACGATTTCACCCCATTGATGACCTGTTAT
TTAACAGATTCGCTGGATCCTAATGAGCTGGAGCGCGGATTTAACGAAGGCGTGTTCACC
GCTGCAAAACTTTACCCGGCAAACGCAACCACTAACTCCAGCCACGGCGTGACGTCAATT
GACGCAATCATGCCGGTACTTGAGCGCATGGAAAAAATCGGTATGCCGCTACTGGTGCAT
GGTGAAGTGACACATGCAGATATCGACATTTTTGATCGTGAAGCGCGCTTTATAGAAAGC
GTGATGGAACCTCTGCGCCAGCGCCTGACTGCGCTGAAAGTCGTTTTTGAGCACATCACC
ACCAAAGATGCTGCCGACTATGTCCGTGACGGAAATGAACGGCTGGCTGCCACCATCACT
CCGCAGCATCTGATGTTTAACCGCAACCATATGCTGGTTGGAGGCGTGCGTCCGCACCTG
TATTGTCTACCCATCCTCAAACGTAATATTCACCAACAGGCATTGCGTGAACTGGTCGCC
AGCGGTTTTAATCGAGTATTCCTCGGTACGGATTCTGCGCCACATGCACGTCATCGCAAA
GAGAGCAGTTGCGGCTGCGCGGGCTGCTTCAACGCCCCAACCGCGCTGGGCAGTTACGCT
ACCGTCTTTGAAGAAATGAATGCTTTGCAGCACTTTGAAGCATTCTGTTCTGTAAACGGC
CCGCAGTTCTATGGGTTGCCGGTCAACGACACATTCATCGAACTGGTACGTGAAGAGCAA
CAGGTTGCTGAAAGCATCGCACTGACTGATGACACGCTGGTGCCATTCCTCGCCGGGGAA
ACGGTACGCTGGTCCGTTAAACAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:348
Protein Molecular Weight:38827
Protein Theoretical pI:6
PDB File:1XGE
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Dihydroorotase
MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMPNLAPPVTTVEAAVAYR
QRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAAKLYPANATTNSSHGVTSI
DAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIESVMEPLRQRLTALKVVFEHIT
TKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRPHLYCLPILKRNIHQQALRELVA
SGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTALGSYATVFEEMNALQHFEAFCSVNG
PQFYGLPVNDTFIELVREEQQVAESIALTDDTLVPFLAGETVRWSVKQ
References
External Links:
ResourceLink
Uniprot ID:P05020
Uniprot Name:PYRC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651523
PDB ID:1XGE
Ecogene ID:EG10806
Ecocyc:EG10806
ColiBase:b1062
Kegg Gene:b1062
EchoBASE ID:EB0799
CCDB:PYRC_ECOLI
BacMap:16129025
General Reference:
  • Backstrom, D., Sjoberg, R. M., Lundberg, L. G. (1986). "Nucleotide sequence of the structural gene for dihydroorotase of Escherichia coli K12." Eur J Biochem 160:77-82. Pubmed: 2876892
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Brown, D. C., Collins, K. D. (1991). "Dihydroorotase from Escherichia coli. Substitution of Co(II) for the active site Zn(II)." J Biol Chem 266:1597-1604. Pubmed: 1671037
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Thoden, J. B., Phillips, G. N. Jr, Neal, T. M., Raushel, F. M., Holden, H. M. (2001). "Molecular structure of dihydroorotase: a paradigm for catalysis through the use of a binuclear metal center." Biochemistry 40:6989-6997. Pubmed: 11401542
  • Wilson, H. R., Chan, P. T., Turnbough, C. L. Jr (1987). "Nucleotide sequence and expression of the pyrC gene of Escherichia coli K-12." J Bacteriol 169:3051-3058. Pubmed: 2885307