Leucine-specific-binding protein (P04816)

Identification
Name:Leucine-specific-binding protein
Synonyms:
  • L-BP
  • LS-BP
Gene Name:livK
Enzyme Class:Not Available
Biological Properties
General Function:Involved in amino acid transport
Specific Function:This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb
1.0Adenosine triphosphate + 1.0Water + 1.0L-Leucine → 1.0ADP + 1.0Hydrogen ion + 1.0L-Leucine + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00687L-LeucineMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Process
amine transport
amino acid transport
establishment of localization
transport
Gene Properties
Blattner:b3458
Gene OrientationCounterclockwise
Centisome Percentage:77.47
Left Sequence End3594474
Right Sequence End3595583
Gene Sequence:
>1110 bp
GTGAGTTTTACTCAATTAATGAGAGTTTCTATGAAAATAGCGTTTATCGGCGAAGCTGTT
TCTGGATTTGGCGGCATGGAAACTGTCATCCGTGATGTTATTACTACCTTCAGGCAACAA
CATATCCAAAGTGAAATGTTTTTCTTTTGTCGTAATGACAAAATGGATAAGGGATGGCTG
GAAGGTATTAAATACTCATGTTCATTTTCCAATATTCGTTTAGGTTTTTTGCGTCGGGCG
AAACATATTCATGCTCTAAGTAAATGGCTTCAGGAGTACCAACCAGATATCGTTATTTGT
ATTGATGTGATCTCCTGCCTGTTTGCCGCAAAAGCACGTAAAAAATCAGGAATTGATATG
CCAGTATTTTCATGGCCACATTTTTCTTTAGATCACAAAAAACATGCTGAATATATCACC
TGTGCCGACTATCATTTAGCAATCAGCTCTGGCATTAAACAGCAAATGATTAATCGAGGT
GTAGCAGAATCGACGATAAATGTTATTTTTAATCCCGTCGAAACTAAAGACTCAGTCATT
CCCGCGCCCGAGGAAGGTGAAACGGCAACGTTTATTTATGTTGGGCGTATGAAATTTGAA
GGCCAGAAAAGAGTTAAAGACCTGCTTGATGGCTTATCACAAGCGAAAGGTAACTGGAAA
CTCCATGTATTGGGTGATGGCTCCGATTTCGAAAAATGCCAGGCTTATGGTAGAGAATTA
AATATTGATGATCGAATTGTCTGGTATGGTTGGCAACAATATCCCTGGGAATTGGTCCAG
CAAGATATTGAAAAAGTGAGTGCGTTATTGCTCACGTCATCTTTTGAAGGTTTCCCAATG
ACCCTACTTGAAGCCTTGTCGTGGGGGATTCCTTGTATCAGTGCCGATTGTGTCTCCGGG
CCTGCTGACATTATTCAACCTGATGTCAATGGCCATCTGTATCAACCGGGAGATATTGCT
GGTTTTGTAACTCTTCTAAATAAATATATAGCAGGTGAAATTCATATCGAACATGAAAAA
ATTCCTGCGTCGATCGATGAATTTTACCAATCGAAGTATTACGATAGATTACATAAGGTA
ATTATTTCTGCTATTTCCCGGAGGAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:369
Protein Molecular Weight:39378
Protein Theoretical pI:5
PDB File:1USK
Signaling Regions:
  • 1-23
Transmembrane Regions:
  • None
Protein Sequence:
>Leucine-specific-binding protein
MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDMEFNGARQAIKDINAKG
GIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCSSSTQPASDIYEDEGILMI
SPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVKPQRIAIIHDKQQYGEGLARS
VQDGLKAANANVVFFDGITAGEKDFSALIARLKKENIDFVYYGGYYPEMGQMLRQARSVG
LKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYDQDPANQGIVDALKADKKDPSGPYV
WITYAAVQSLATALERTGSDEPLALVKDLKANGANTVIGPLNWDEKGDLKGFDFGVFQWH
ADGSSTAAK
References
External Links:
ResourceLink
Uniprot ID:P04816
Uniprot Name:LIVK_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676414
PDB ID:1USK
Ecogene ID:EG10540
Ecocyc:EG10540
ColiBase:b3458
Kegg Gene:b3458
EchoBASE ID:EB0535
CCDB:LIVK_ECOLI
BacMap:16131330
General Reference:
  • Adams, M. D., Wagner, L. M., Graddis, T. J., Landick, R., Antonucci, T. K., Gibson, A. L., Oxender, D. L. (1990). "Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli." J Biol Chem 265:11436-11443. Pubmed: 2195019
  • Antonucci, T. K., Landick, R., Oxender, D. L. (1985). "The leucine binding proteins of Escherichia coli as models for studying the relationships between protein structure and function." J Cell Biochem 29:209-216. Pubmed: 4077929
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Landick, R., Oxender, D. L. (1985). "The complete nucleotide sequences of the Escherichia coli LIV-BP and LS-BP genes. Implications for the mechanism of high-affinity branched-chain amino acid transport." J Biol Chem 260:8257-8261. Pubmed: 3891753
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Oxender, D. L., Anderson, J. J., Daniels, C. J., Landick, R., Gunsalus, R. P., Zurawski, G., Yanofsky, C. (1980). "Amino-terminal sequence and processing of the precursor of the leucine-specific binding protein, and evidence for conformational differences between the precursor and the mature form." Proc Natl Acad Sci U S A 77:2005-2009. Pubmed: 6990419
  • Sack, J. S., Trakhanov, S. D., Tsigannik, I. H., Quiocho, F. A. (1989). "Structure of the L-leucine-binding protein refined at 2.4 A resolution and comparison with the Leu/Ile/Val-binding protein structure." J Mol Biol 206:193-207. Pubmed: 2649683
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620