DNA polymerase III subunit epsilon (P03007)

Identification
Name:DNA polymerase III subunit epsilon
Synonyms:Not Available
Gene Name:dnaQ
Enzyme Class:
Biological Properties
General Function:Involved in DNA binding
Specific Function:DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dATP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dGTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0dCTP + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0DNA
1.0Thymidine 5'-triphosphate + 1.0DNA ↔ 1.0Pyrophosphate + 1.0DNA
ReactionCard
1.0Deoxynucleoside triphosphate+1.0DNA ↔ 1.0Thumb
1.0Deoxynucleoside triphosphate + 1.0DNA ↔ 1.0Pyrophosphate
ReactionCard
EcoCyc Reactions:
1.0DNAn ? 1.0a nucleoside monophosphate
1.0DNAn ? 1.0a nucleoside monophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01532dATPMetaboCard
ECMDB00998dCTPMetaboCard
ECMDB01440dGTPMetaboCard
ECMDB04142PyrophosphateMetaboCard
ECMDB01342Thymidine 5'-triphosphateMetaboCard
GO Classification:
Component
cell part
intracellular
Function
binding
catalytic activity
DNA binding
DNA polymerase activity
DNA-directed DNA polymerase activity
exonuclease activity
hydrolase activity
hydrolase activity, acting on ester bonds
nuclease activity
nucleic acid binding
nucleotidyltransferase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b0215
Gene OrientationClockwise
Centisome Percentage:5.09
Left Sequence End236067
Right Sequence End236798
Gene Sequence:
>732 bp
ATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAG
ATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGCCGTTGAAGTGGTGAAC
CGTCGCCTGACGGGCAATAACTTCCATGTTTATCTCAAACCCGATCGGCTGGTGGATCCG
GAAGCCTTTGGCGTACATGGTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCC
GAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCA
GCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAG
ACCAATACTTTCTGTAAGGTCACCGATAGCCTTGCGGTGGCGAGGAAAATGTTTCCCGGT
AAGCGCAACAGCCTCGATGCGTTATGTGCTCGCTACGAAATAGATAACAGTAAACGAACG
CTGCACGGGGCATTACTCGATGCCCAGATCCTTGCGGAAGTTTATCTGGCGATGACCGGT
GGTCAAACGTCGATGGCTTTTGCGATGGAAGGAGAGACACAACAGCAACAAGGTGAAGCA
ACAATTCAGCGCATTGTACGTCAGGCAAGTAAGTTACGCGTTGTTTTTGCGACAGATGAA
GAGATTGCAGCTCATGAAGCCCGTCTCGATCTGGTGCAGAAGAAAGGCGGAAGTTGCCTC
TGGCGAGCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:243
Protein Molecular Weight:27099
Protein Theoretical pI:6
PDB File:1J54
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA polymerase III subunit epsilon
MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLTGNNFHVYLKPDRLVDP
EAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAFDIGFMDYEFSLLKRDIPK
TNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRTLHGALLDAQILAEVYLAMTG
GQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFATDEEIAAHEARLDLVQKKGGSCL
WRA
References
External Links:
ResourceLink
Uniprot ID:P03007
Uniprot Name:DPO3E_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902952
PDB ID:1J54
Ecogene ID:EG10243
Ecocyc:EG10243
ColiBase:b0215
Kegg Gene:b0215
EchoBASE ID:EB0239
CCDB:DPO3E_ECOLI
BacMap:16128202
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Cox, E. C., Horner, D. L. (1986). "DNA sequence and coding properties of mutD(dnaQ) a dominant Escherichia coli mutator gene." J Mol Biol 190:113-117. Pubmed: 3023634
  • Hamdan, S., Carr, P. D., Brown, S. E., Ollis, D. L., Dixon, N. E. (2002). "Structural basis for proofreading during replication of the Escherichia coli chromosome." Structure 10:535-546. Pubmed: 11937058
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Maki, H., Horiuchi, T., Sekiguchi, M. (1983). "Structure and expression of the dnaQ mutator and the RNase H genes of Escherichia coli: overlap of the promoter regions." Proc Natl Acad Sci U S A 80:7137-7141. Pubmed: 6316347
  • O'Donnell, M. (1992). "Accessory protein function in the DNA polymerase III holoenzyme from E. coli." Bioessays 14:105-111. Pubmed: 1575709
  • Takano, K., Nakabeppu, Y., Maki, H., Horiuchi, T., Sekiguchi, M. (1986). "Structure and function of dnaQ and mutD mutators of Escherichia coli." Mol Gen Genet 205:9-13. Pubmed: 3540531