Glycyl-tRNA synthetase alpha subunit (P00960)

Identification
Name:Glycyl-tRNA synthetase alpha subunit
Synonyms:
  • Glycine--tRNA ligase alpha subunit
  • GlyRS
Gene Name:glyQ
Enzyme Class:
Biological Properties
General Function:Involved in nucleotide binding
Specific Function:ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0tRNA(Gly)+1.0tRNA(Gly) ↔ 1.0Thumb+1.0Glycyl-tRNA(Gly)+1.0Thumb+1.0Glycyl-tRNA(Gly)
1.0Adenosine triphosphate + 1.0Glycine + 1.0tRNA(Gly) + 1.0tRNA(Gly) ↔ 1.0Adenosine monophosphate + 1.0Glycyl-tRNA(Gly) + 1.0Pyrophosphate + 1.0Glycyl-tRNA(Gly)
ReactionCard
1.0Thumb+1.0Thumb+1.0tRNA(Gly) ↔ 1.0Thumb+1.0Thumb+1.0Glycyl-tRNA(Gly)
1.0Adenosine triphosphate + 1.0Glycine + 1.0tRNA(Gly) ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0Glycyl-tRNA(Gly)
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0tRNA(gly) → 1.0Thumb+1.0Pyrophosphate+1.0Glycyl-tRNA(Gly)
1.0Glycine + 1.0Adenosine triphosphate + 1.0Hydrogen ion + 1.0tRNA(gly) → 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0Glycyl-tRNA(Gly)
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb+1.0tRNA(Gly) → 1.0Thumb+1.0Thumb+1.0glycyl-tRNA(Gly)
1.0Adenosine triphosphate + 1.0Glycine + 1.0tRNA(Gly) → 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.0glycyl-tRNA(Gly)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB00123GlycineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
aminoacyl-tRNA ligase activity
ATP binding
binding
catalytic activity
glycine-tRNA ligase activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
ligase activity, forming carbon-oxygen bonds
nucleoside binding
nucleotide binding
purine nucleoside binding
Process
biosynthetic process
cellular macromolecule biosynthetic process
cellular macromolecule metabolic process
glycyl-tRNA aminoacylation
macromolecule biosynthetic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
RNA metabolic process
translation
tRNA aminoacylation
tRNA aminoacylation for protein translation
tRNA metabolic process
Gene Properties
Blattner:b3560
Gene OrientationCounterclockwise
Centisome Percentage:80.23
Left Sequence End3722430
Right Sequence End3723341
Gene Sequence:
>912 bp
ATGCGACTATTTAACTGGCGACGTCAGGCGGTGTTAAACGCCATGCCACTGGTCAAACCT
GACCAGGTACGTACACCGTGGCATGAATTCTGGCGACGATTTCGCCGTCAGCATATGGCG
ATGACCGCCGCATTATTCGTTATTTTATTGATTGTGGTGGCCATTTTTGCACGCTGGATC
GCTCCCTATGACGCCGAAAATTATTTTGATTATGACAATCTGAATAACGGACCTTCTTTG
CAGCACTGGTTTGGCGTCGATTCACTGGGGCGTGACATTTTCAGCCGTGTCCTGGTTGGT
GCGCAAATCTCGCTGGCGGCGGGCGTGTTTGCCGTGTTTATCGGTGCGGCGATCGGGACG
TTGCTGGGCTTGCTCGCTGGATATTATGAAGGCTGGTGGGATCGGCTGATCATGCGCATT
TGCGATGTGCTGTTTGCCTTCCCGGGTATTTTACTGGCGATCGCTGTTGTTGCGGTGTTG
GGAAGCGGCATTGCTAACGTGATTATTGCAGTCGCCATTTTTTCCATCCCCGCGTTTGCC
CGCCTGGTGCGCGGCAACACGCTGGTGTTGAAACAGCAAACCTTTATTGAGTCAGCACGC
AGTATTGGTGCCAGCGATATGACCGTTTTGTTGCGTCATATCCTGCCTGGGACCGTCTCT
TCTATCGTGGTGTTTTTCACCATGCGCATTGGTACCTCGATTATCTCTGCCGCCAGCCTC
TCATTTCTCGGCCTCGGTGCGCAGCCGCCGACACCAGAGTGGGGAGCAATGCTCAATGAG
GCTCGAGCGGATATGGTTATCGCGCCGCATGTCGCTGTTTTTCCGGCCCTGGCTATTTTT
CTGACCGTACTGGCGTTCAATTTGTTGGGCGATGGTTTACGCGATGCGCTGGATCCGAAA
ATTAAAGGATAG
Protein Properties
Pfam Domain Function:
Protein Residues:303
Protein Molecular Weight:34774
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glycyl-tRNA synthetase alpha subunit
MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMTCLRELGPEPMAAAYVQ
PSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSLKELGMDPTIHDIRFVEDN
WENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVTGEITYGLERLAMYIQGVDSV
YDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDFLFTCFEQYEKEAQQLLALENPL
PLPAYERILKAAHSFNLLDARKAISVTERQRYILRIRTLTKAVAEAYYASREALGFPMCN
KDK
References
External Links:
ResourceLink
Uniprot ID:P00960
Uniprot Name:SYGA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062409
Ecogene ID:EG10409
Ecocyc:EG10409
ColiBase:b3560
Kegg Gene:b3560
EchoBASE ID:EB0404
CCDB:SYGA_ECOLI
BacMap:16131431
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Keng, T., Webster, T. A., Sauer, R. T., Schimmel, P. (1982). "Gene for Escherichia coli glycyl-tRNA synthetase has tandem subunit coding regions in the same reading frame." J Biol Chem 257:12503-12508. Pubmed: 6290471
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Webster, T. A., Gibson, B. W., Keng, T., Biemann, K., Schimmel, P. (1983). "Primary structures of both subunits of Escherichia coli glycyl-tRNA synthetase." J Biol Chem 258:10637-10641. Pubmed: 6309809