Xylose isomerase (P00944)

Identification
Name:Xylose isomerase
Synonyms:
  • D-xylulose keto-isomerase
Gene Name:xylA
Enzyme Class:
Biological Properties
General Function:Involved in xylose isomerase activity
Specific Function:D-xylose = D-xylulose
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0D-Xylose ↔ 1.0D-Xylulose
ReactionCard
1.0Thumb ↔ 1.0D-Fructose
1.0alpha-D-Glucose ↔ 1.0D-Fructose
ReactionCard
SMPDB Reactions:
1.0β-D-xylopyranose ↔ 1.0Thumb
1.0β-D-xylopyranose ↔ 1.0L-Threo-2-pentulose
ReactionCard
Complex Reactions:
1.0Thumb ↔ 1.0Thumb
1.0D-Glucose ↔ 1.0D-Fructose
ReactionCard
1.0Thumb → 1.0Thumb
1.0D-Xylose → 1.0D-Xylulose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03345alpha-D-GlucoseMetaboCard
ECMDB00660D-FructoseMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB00098D-XyloseMetaboCard
ECMDB00654D-XyluloseMetaboCard
ECMDB00751L-Threo-2-pentuloseMetaboCard
GO Classification:
Function
catalytic activity
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, interconverting aldoses and ketoses
isomerase activity
xylose isomerase activity
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3565
Gene OrientationCounterclockwise
Centisome Percentage:80.34
Left Sequence End3727466
Right Sequence End3728788
Gene Sequence:
>1323 bp
ATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGACTTC
TCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGC
GAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATGTGAGT
GATGCGTTTGAGCAAACCAGCGAAACCATCGGCGTGCGCGCCAATAACGCGATCAACGAC
ATGGTGCGTCAACGTCTGCTGAACCGCTTTACCAGCGAGCAGGCGGAAGGGAACGCAATT
TACCGTCTGACGCCGCTCGGCATCGGCATTACTGACTACTACATCCGTCAGCGCGAGTTT
TCTACGCTGCGTCTTTCTATGCAGTTGTCGATTGTGGCGGGTGAGCTCAAACGCGCAGCA
GATGCCGCCGAAGAGGGCGGTGATGAATTTCACTGGCACCGTAATGTCTATGCGCCACTG
AAATATTCGGTAGCAGAAATTTTCGACAGTATCGACCTGACGCAACGTCTGATGGACGAA
CAGCAGCAGCAGGTGAAGGACGATATCGCCCAGTTGCTGAACAAAGACTGGCGGGCGGCG
ATTTCCAGCTGTGAATTGTTGCTTTCGGAAACTTCCGGAACGCTGCGTGAATTGCAGGAT
ACGCTGGAAGCGGCAGGCGACAAATTGCAGGCTAATCTGTTGCGCATTCAGGATGCGACG
ATGACCCATGACGATCTGCATTTCGTCGATCGTCTGGTGTTCGATCTGCAGAGCAAACTC
GATCGTATTATCAGTTGGGGCCAGCAATCCATCGACTTGTGGATTGGCTACGACCGCCAC
GTACACAAATTTATTCGTACCGCGATCGATATGGATAAAAACCGCGTCTTTGCTCAGCGG
TTACGTCAGTCGGTACAAACCTATTTTGATGAGCCGTGGGCGCTAACTTATGCCAATGCC
GATCGTCTGCTGGATATGCGTGACGAAGAGATGGCACTGCGCGATGAAGAAGTGACTGGG
GAACTTCCTGAGGATCTGGAATACGAAGAGTTTAACGAGATCCGCGAACAGCTGGCGGCG
ATCATCGAAGAACAACTTGCCGTGTACAAAACCAGACAAGTGCCGCTGGATCTTGGTCTG
GTGGTACGCGAATATCTGTCACAGTATCCGCGTGCACGTCACTTTGACGTTGCGCGTATT
GTTATTGATCAGGCGGTACGTCTTGGCGTAGCGCAAGCAGATTTCACCGGACTGCCAGCG
AAATGGCAGCCGATTAATGATTACGGAGCCAAGGTACAGGCGCATGTCATCGACAAATAT
TGA
Protein Properties
Pfam Domain Function:
Protein Residues:440
Protein Molecular Weight:49742
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Xylose isomerase
MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFAACYWHTFCWNGADMF
GVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDVDVSPEGASLKEYINNFA
QMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDPEVFSWAATQVVTAMEATHKL
GGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEHKHKIGFQGTLLIEPKPQEPTK
HQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGHSFHHEIATAIALGLFGSVDANRG
DAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGGLNFDAKVRRQSTDKYDLFYGHIGAM
DTMALALKIAARMIEDGELDKRIAQRYSGWNSELGQQILKGQMSLADLAKYAQEHHLSPV
HQSGRQEQLENLVNHYLFDK
References
External Links:
ResourceLink
Uniprot ID:P00944
Uniprot Name:XYLA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651446
Ecogene ID:EG11074
Ecocyc:EG11074
ColiBase:b3565
Kegg Gene:b3565
EchoBASE ID:EB1067
CCDB:XYLA_ECOLI
BacMap:16131436
General Reference:
  • Batt, C. A., Jamieson, A. C., Vandeyar, M. A. (1990). "Identification of essential histidine residues in the active site of Escherichia coli xylose (glucose) isomerase." Proc Natl Acad Sci U S A 87:618-622. Pubmed: 2405386
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Briggs, K. A., Lancashire, W. E., Hartley, B. S. (1984). "Molecular cloning, DNA structure and expression of the Escherichia coli D-xylose isomerase." EMBO J 3:611-616. Pubmed: 6325179
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hou, Y. M., Zhang, Q. J., Wang, S. J. (1990). "Sequence analysis of D-xylose isomerase gene from Escherichia coli." Chin J Biotechnol 6:269-277. Pubmed: 2132127
  • Lawlis, V. B., Dennis, M. S., Chen, E. Y., Smith, D. H., Henner, D. J. (1984). "Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli." Appl Environ Microbiol 47:15-21. Pubmed: 6320721
  • Schellenberg, G. D., Sarthy, A., Larson, A. E., Backer, M. P., Crabb, J. W., Lidstrom, M., Hall, B. D., Furlong, C. E. (1984). "Xylose isomerase from Escherichia coli. Characterization of the protein and the structural gene." J Biol Chem 259:6826-6832. Pubmed: 6327696
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620