Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (P00887)

Identification
Name:Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive
Synonyms:
  • 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase
  • DAHP synthase
  • Phospho-2-keto-3-deoxyheptonate aldolase
Gene Name:aroH
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP)
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Metabolic pathways eco01100
  • Phenylalanine, tyrosine and tryptophan biosynthesis ec00400
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Erythrose 4-phosphate + 1.0Water + 1.0Phosphoenolpyruvic acid ↔ 1.02-Dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + 1.0Phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0D-Erythrose 4-phosphate + 1.0Water + 1.0Phosphoenolpyruvic acid → 1.0Phosphate + 1.03-deoxy-D-arabino-heptulosonate-7-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Erythrose 4-phosphate + 1.0Water + 1.0Phosphoenolpyruvic acid ↔ 1.02-Dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + 1.0Phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200392-Dehydro-3-deoxy-D-arabino-heptonate 7-phosphateMetaboCard
ECMDB241823-deoxy-D-arabino-heptulosonate-7-phosphateMetaboCard
ECMDB02649D-Erythrose 4-phosphateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00263Phosphoenolpyruvic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
3-deoxy-7-phosphoheptulonate synthase activity
catalytic activity
transferase activity
transferase activity, transferring alkyl or aryl (other than methyl) groups
Process
aromatic amino acid family biosynthetic process
biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
Gene Properties
Blattner:b1704
Gene OrientationClockwise
Centisome Percentage:38.50
Left Sequence End1786459
Right Sequence End1787505
Gene Sequence:
>1047 bp
ATGAACAGAACTGACGAACTCCGTACTGCGCGTATTGAGAGCCTGGTAACGCCCGCCGAA
CTCGCGCTACGGTATCCCGTAACGCCTGGCGTCGCCACCCATGTCACCGACTCCCGCCGC
AGAATTGAAAAAATACTGAATGGTGAAGATAAGCGACTGTTGGTCATTATTGGCCCCTGC
TCGATCCACGATCTCACCGCTGCAATGGAGTACGCCACCCGTCTGCAGTCGCTGCGCAAC
CAGTACCAGTCACGGCTGGAAATCGTAATGCGCACCTATTTTGAAAAACCACGAACTGTT
GTCGGCTGGAAAGGACTAATCTCCGATCCAGATTTAAACGGCAGCTATCGGGTAAATCAC
GGTCTGGAGCTGGCGCGCAAATTACTTTTACAGGTAAATGAGCTGGGCGTCCCAACCGCG
ACCGAGTTCCTCGATATGGTGACCGGTCAGTTTATTGCTGATTTAATCAGTTGGGGCGCG
ATTGGCGCACGTACTACCGAAAGTCAGATCCACCGCGAAATGGCTTCGGCACTCTCCTGT
CCGGTAGGTTTTAAAAATGGTACCGATGGCAATACGCGGATTGCTGTGGATGCTATCCGC
GCAGCCCGCGCCAGCCATATGTTCCTCTCGCCAGACAAAAATGGTCAGATGACCATCTAT
CAGACCAGCGGCAACCCGTATGGCCACATTATTATGCGTGGCGGCAAAAAACCGAATTAT
CATGCCGATGATATCGCCGCAGCCTGCGATACGCTGCACGAGTTTGATTTACCTGAACAT
CTGGTGGTGGATTTCAGCCACGGTAACTGCCAGAAGCAGCACCGTCGCCAGTTAGAAGTT
TGTGAGGATATTTGTCAGCAAATCCGCAATGGCTCTACGGCGATTGCTGGAATTATGGCG
GAAAGTTTCCTGCGCGAAGGAACGCAAAAAATCGTCGGCAGTCAGCCGCTCACTTACGGT
CAATCCATTACCGACCCGTGTCTGGGCTGGGAGGATACCGAACGCCTGGTCGAAAAACTC
GCCTCTGCGGTAGATACCCGCTTCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:348
Protein Molecular Weight:38735
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive
MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPC
SIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRVNH
GLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGAIGARTTESQIHREMASALSC
PVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNY
HADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMA
ESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTERLVEKLASAVDTRF
References
External Links:
ResourceLink
Uniprot ID:P00887
Uniprot Name:AROH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742785
Ecogene ID:EG10080
Ecocyc:EG10080
ColiBase:b1704
Kegg Gene:b1704
EchoBASE ID:EB0078
CCDB:AROH_ECOLI
BacMap:16129660
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Hudson, G. S., Rellos, P., Davidson, B. E. (1991). "Two promoters control the aroH gene of Escherichia coli." Gene 102:87-91. Pubmed: 1677907
  • Ray, J. M., Yanofsky, C., Bauerle, R. (1988). "Mutational analysis of the catalytic and feedback sites of the tryptophan-sensitive 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase of Escherichia coli." J Bacteriol 170:5500-5506. Pubmed: 2903857
  • Zurawski, G., Gunsalus, R. P., Brown, K. D., Yanofsky, C. (1981). "Structure and regulation of aroH, the structural gene for the tryptophan-repressible 3-deoxy-D-arabino-heptulosonic acid-7-phosphate synthetase of Escherichia coli." J Mol Biol 145:47-73. Pubmed: 6167722