2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (Q46893)

Identification
Name:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Synonyms:
  • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase
  • CDP-ME synthase
  • MEP cytidylyltransferase
  • MCT
Gene Name:ispD
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)
Cellular Location:Not Available
SMPDB Pathways:
  • Operon: cytidyl pyrophosphate biosynthesis of isoprenoids PW000988
  • Secondary metabolites: isoprenoid biosynthesis (nonmevalonate pathway) PW000975
  • Secondary metabolites: methylerythritol phosphate and polyisoprenoid biosynthesis PW000958
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-C-Methyl-D-erythritol-4-phosphate + 1.0Cytidine triphosphate + 1.0Hydrogen ion ↔ 1.04-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol + 1.0Pyrophosphate
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-C-Methyl-D-erythritol-4-phosphate + 1.0Cytidine triphosphate ↔ 1.04-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol + 1.0Pyrophosphate
ReactionCard
SMPDB Reactions:
1.02-C-methyl-D-erythritol 4-phosphate+1.0Thumb+1.0Thumb → 1.0Pyrophosphate+1.04-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol+1.0Thumb
1.02-C-methyl-D-erythritol 4-phosphate + 1.0Cytidine triphosphate + 1.0Hydrogen ion → 1.0Pyrophosphate + 1.04-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + 1.04-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0D-Ribulose 5-phosphate → 1.01-Deoxy-L-glycero-tetrulose 4-phosphate + 1.0Formic acid + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-C-Methyl-D-erythritol-4-phosphate + 1.0Cytidine triphosphate + 1.0Hydrogen ion ↔ 1.04-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol + 1.0Pyrophosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB231221-Deoxy-L-glycero-tetrulose 4-phosphateMetaboCard
ECMDB041042-C-Methyl-D-erythritol-4-phosphateMetaboCard
ECMDB200834-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritolMetaboCard
ECMDB00082Cytidine triphosphateMetaboCard
ECMDB04148D-Ribulose 5-phosphateMetaboCard
ECMDB00142Formic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04142PyrophosphateMetaboCard
GO Classification:
Function
catalytic activity
Process
cellular lipid metabolic process
isoprenoid biosynthetic process
isoprenoid metabolic process
lipid metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b2747
Gene OrientationCounterclockwise
Centisome Percentage:61.85
Left Sequence End2869802
Right Sequence End2870512
Gene Sequence:
>711 bp
ATGGCAACCACTCATTTGGATGTTTGCGCCGTGGTTCCGGCGGCCGGATTTGGCCGTCGA
ATGCAAACGGAATGTCCTAAGCAATATCTCTCAATCGGTAATCAAACCATTCTTGAACAC
TCGGTGCATGCGCTGCTGGCGCATCCCCGGGTGAAACGTGTCGTCATTGCCATAAGTCCT
GGCGATAGCCGTTTTGCACAACTTCCTCTGGCGAATCATCCGCAAATCACCGTTGTAGAT
GGCGGTGATGAGCGTGCCGATTCCGTGCTGGCAGGTCTGAAAGCCGCTGGCGACGCGCAG
TGGGTATTGGTGCATGACGCCGCTCGTCCTTGTTTGCATCAGGATGACCTCGCGCGATTG
TTGGCGTTGAGCGAAACCAGCCGCACGGGGGGGATCCTCGCCGCACCAGTGCGCGATACT
ATGAAACGTGCCGAACCGGGCAAAAATGCCATTGCTCATACCGTTGATCGCAACGGCTTA
TGGCACGCGCTGACGCCGCAATTTTTCCCTCGTGAGCTGTTACATGACTGTCTGACGCGC
GCTCTAAATGAAGGCGCGACTATTACCGACGAAGCCTCGGCGCTGGAATATTGCGGATTC
CATCCTCAGTTGGTCGAAGGCCGTGCGGATAACATTAAAGTCACGCGCCCGGAAGATTTG
GCACTGGCCGAGTTTTACCTCACCCGAACCATCCATCAGGAGAATACATAA
Protein Properties
Pfam Domain Function:
Protein Residues:236
Protein Molecular Weight:25737
Protein Theoretical pI:7
PDB File:1INI
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHALLAHPRVKRVVIAISP
GDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWVLVHDAARPCLHQDDLARL
LALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGLWHALTPQFFPRELLHDCLTR
ALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPEDLALAEFYLTRTIHQENT
References
External Links:
ResourceLink
Uniprot ID:Q46893
Uniprot Name:ISPD_ECOLI
GenBank Gene ID:AB037143
Genebank Protein ID:6978968
PDB ID:1INI
Ecogene ID:EG13110
Ecocyc:EG13110
ColiBase:b2747
Kegg Gene:b2747
EchoBASE ID:EB2913
CCDB:ISPD_ECOLI
BacMap:16130654
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kemp, L. E., Bond, C. S., Hunter, W. N. (2001). "Crystallization and preliminary X-ray diffraction studies of recombinant Escherichia coli 4-diphosphocytidyl-2-C-methyl-D-erythritol synthetase." Acta Crystallogr D Biol Crystallogr 57:1189-1191. Pubmed: 11468415
  • Kemp, L. E., Bond, C. S., Hunter, W. N. (2003). "Structure of a tetragonal crystal form of Escherichia coli 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase." Acta Crystallogr D Biol Crystallogr 59:607-610. Pubmed: 12595740
  • Richard, S. B., Bowman, M. E., Kwiatkowski, W., Kang, I., Chow, C., Lillo, A. M., Cane, D. E., Noel, J. P. (2001). "Structure of 4-diphosphocytidyl-2-C- methylerythritol synthetase involved in mevalonate- independent isoprenoid biosynthesis." Nat Struct Biol 8:641-648. Pubmed: 11427897
  • Rohdich, F., Wungsintaweekul, J., Fellermeier, M., Sagner, S., Herz, S., Kis, K., Eisenreich, W., Bacher, A., Zenk, M. H. (1999). "Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol." Proc Natl Acad Sci U S A 96:11758-11763. Pubmed: 10518523
  • Sauret-Gueto, S., Ramos-Valdivia, A., Ibanez, E., Boronat, A., Rodriguez-Concepcion, M. (2003). "Identification of lethal mutations in Escherichia coli genes encoding enzymes of the methylerythritol phosphate pathway." Biochem Biophys Res Commun 307:408-415. Pubmed: 12859972