Identification
Name:D-phenylhydantoinase
Synonyms:
  • Hydantoin-utilizing enzyme hyuA
Gene Name:hyuA
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
Specific Function:Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives with an aromatic side chains at the 5'-position. Has no activity on dihydropyrimidines. The physiological function is unknown
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb1.0Thumb
1.0Thumb+1.0Thumb1.0Thumb
1.05,6-Dihydro-5-fluorouracil+1.0Thumb1.0alpha-Fluoro-beta-ureidopropionic acid
1.05,6-Dihydro-5-fluorouracil + 1.0Water ↔ 1.0alpha-Fluoro-beta-ureidopropionic acid
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0phenylureidoacetic acid
1.0Phenylhydantoin + 1.0Water ↔ 1.0phenylureidoacetic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00079DihydrothymineMetaboCard
ECMDB00076DihydrouracilMetaboCard
ECMDB21541HydroxyphenylhydantoinMetaboCard
ECMDB20599PhenylhydantoinMetaboCard
ECMDB02031Ureidoisobutyric acidMetaboCard
ECMDB00026Ureidopropionic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
Gene Properties
Blattner:b2873
Gene OrientationClockwise
Centisome Percentage:64.83
Left Sequence End3008050
Right Sequence End3009435
Gene Sequence:
>1386 bp
ATGCTAAAAATCTTCAATACTCTGACACGCCAAAAAGAGGAATTTAAGCCTATTCACGCC
GGGGAAGTCGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCAC
GGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAA
CTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAA
AATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTT
GATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGACGCACCATATCGCAGAA
ATTATTGAACTCACTGAACAACTGATCGCCAAAGGTCACGCTTATGTGGCGGACAACGGC
GACGTGATGTTCGACGTCCCGACCGATCCAACTTATGGCGTGCTGTCGCGTCAGGATCTC
GACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGAC
TTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCG
GGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCAC
TTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCG
CAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTG
ATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTG
CTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGC
CAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTAC
ACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCG
CGCTTTATTGAAGCGATGGACGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTT
GATATGGCGCGTGAAGTAAACCGTCTGAAAGCAGAAGATATGGCAGCGGCGAATGCAATG
GCATCTCACCTGCGTAAACTTTCCGCTGTATTGGGCCTGCTGGAGCAAGAACCGGAAGCG
TTCCTGCAAAGCGGCGCGCAGGCAGACGACAGCGAAGTGGCTGAGATTGAAGCGTTAATT
CAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGAT
CGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGT
AAGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:461
Protein Molecular Weight:51025
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>D-phenylhydantoinase
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHT
HFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVI
DYSFHGVIQHINHAILDEIPMIVEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESGALTT
VHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWC
GISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPE
RFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPWEGFT
CQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL
References
External Links:
ResourceLink
Uniprot ID:Q46806
Uniprot Name:PHYDA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674663
Ecogene ID:EG13056
Ecocyc:EG13056
ColiBase:b2873
Kegg Gene:b2873
EchoBASE ID:EB2868
CCDB:PHYDA_ECOLI
BacMap:90111504
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kim, G. J., Lee, D. E., Kim, H. S. (2000). "Functional expression and characterization of the two cyclic amidohydrolase enzymes, allantoinase and a novel phenylhydantoinase, from Escherichia coli." J Bacteriol 182:7021-7028. Pubmed: 11092864