ECMDB

Identification

Name:

D-phenylhydantoinase

Synonyms:

Hydantoin-utilizing enzyme hyuA

Gene Name:

hyuA

Enzyme Class:

3.5.2.2

Biological Properties

General Function:

Involved in hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides

Specific Function:

Catalyzes the stereospecific hydrolysis of the cyclic amide bond of D-hydantoin derivatives with an aromatic side chains at the 5'-position. Has no activity on dihydropyrimidines. The physiological function is unknown

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Collection of Reactions without pathways PW001891
Pantothenate and CoA biosynthesis PW000828
Pyrimidine metabolism PW000942
beta-Alanine metabolism PW000896

KEGG Pathways:

Drug metabolism - other enzymes ec00983
Metabolic pathways eco01100
Pantothenate and CoA biosynthesis ec00770
Pyrimidine metabolism ec00240
beta-Alanine metabolism ec00410

KEGG Reactions:

1.0

↔

1.0

1.0Dihydrouracil + 1.0Water ↔ 1.0Ureidopropionic acid
ReactionCard

1.0

↔

1.0

1.0Dihydrothymine + 1.0Water ↔ 1.0Ureidoisobutyric acid
ReactionCard

1.05,6-Dihydro-5-fluorouracil

1.0

↔

1.0alpha-Fluoro-beta-ureidopropionic acid

1.05,6-Dihydro-5-fluorouracil + 1.0Water ↔ 1.0alpha-Fluoro-beta-ureidopropionic acid
ReactionCard

SMPDB Reactions:

1.0

↔

1.0phenylureidoacetic acid

1.0Phenylhydantoin + 1.0Water ↔ 1.0phenylureidoacetic acid
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB00079	Dihydrothymine	MetaboCard
ECMDB00076	Dihydrouracil	MetaboCard
ECMDB21541	Hydroxyphenylhydantoin	MetaboCard
ECMDB20599	Phenylhydantoin	MetaboCard
ECMDB02031	Ureidoisobutyric acid	MetaboCard
ECMDB00026	Ureidopropionic acid	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Gene Properties

Blattner:

b2873

Gene Orientation

Clockwise

Centisome Percentage:

64.83

Left Sequence End

3008050

Right Sequence End

3009435

Gene Sequence:

>1386 bp
ATGCTAAAAATCTTCAATACTCTGACACGCCAAAAAGAGGAATTTAAGCCTATTCACGCC
GGGGAAGTCGGCATGTACGTGTGTGGAATCACCGTTTACGATCTCTGTCATATCGGTCAC
GGGCGTACCTTTGTTGCTTTTGACGTGGTTGCGCGCTATCTGCGTTTCCTCGGCTATAAA
CTGAAGTATGTGCGCAACATTACCGATATCGACGACAAAATCATCAAACGCGCCAATGAA
AATGGCGAAAGCTTTGTGGCGATGGTGGATCGCATGATCGCCGAAATGCACAAAGATTTT
GATGCTTTGAACATTCTGCGCCCGGATATGGAGCCGCGCGCGACGCACCATATCGCAGAA
ATTATTGAACTCACTGAACAACTGATCGCCAAAGGTCACGCTTATGTGGCGGACAACGGC
GACGTGATGTTCGACGTCCCGACCGATCCAACTTATGGCGTGCTGTCGCGTCAGGATCTC
GACCAGCTGCAGGCAGGCGCGCGCGTTGACGTGGTCGACGACAAACGCAACCCAATGGAC
TTCGTTCTGTGGAAGATGTCGAAAGAGGGCGAACCGAGCTGGCCGTCTCCGTGGGGCGCG
GGTCGTCCTGGCTGGCACATTGAATGTTCGGCAATGAACTGCAAGCAGCTGGGTAACCAC
TTTGATATCCACGGCGGCGGTTCAGACCTGATGTTCCCGCACCACGAAAACGAAATCGCG
CAGTCCACCTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTG
ATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTG
CTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGC
CAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTAC
ACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCTGCCGGTGGCGAAGCGTTTGAAGCG
CGCTTTATTGAAGCGATGGACGACGATTTCAACACCCCGGAAGCCTATTCCGTACTGTTT
GATATGGCGCGTGAAGTAAACCGTCTGAAAGCAGAAGATATGGCAGCGGCGAATGCAATG
GCATCTCACCTGCGTAAACTTTCCGCTGTATTGGGCCTGCTGGAGCAAGAACCGGAAGCG
TTCCTGCAAAGCGGCGCGCAGGCAGACGACAGCGAAGTGGCTGAGATTGAAGCGTTAATT
CAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGAT
CGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGT
AAGTAA

Protein Properties

Pfam Domain Function:

Amidohydro_1 (PF01979 )

Protein Residues:

461

Protein Molecular Weight:

51025

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>D-phenylhydantoinase
MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEIDATGCYVFPGGVDVHT
HFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCRLRHQLEVYRGYAAHKAVI
DYSFHGVIQHINHAILDEIPMIVEEGLSSFKLYLTYQYKLNDDEVLQALRRLHESGALTT
VHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAEAIARMINLAQIAGNAPLYIVHL
SNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYDTEDGMKFILSPPLRNVREQDKLWC
GISDGAIDVVATDHCTFSMAQRLQISKGDFSRCPNGLPGVENRMQLLFSSGVMTGRITPE
RFVELTSAMPARLFGLWPQKGLLAPGSDGDVVIIDPRQSQQIQHRHLHDNADYSPWEGFT
CQGAIVRTLSRGETIFCDGTFTGKAGRGRFLRRKPFVPPVL

References

External Links:

Resource	Link
Uniprot ID:	Q46806
Uniprot Name:	PHYDA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674663
Ecogene ID:	EG13056
Ecocyc:	EG13056
ColiBase:	b2873
Kegg Gene:	b2873
EchoBASE ID:	EB2868
CCDB:	PHYDA_ECOLI
BacMap:	90111504

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Kim, G. J., Lee, D. E., Kim, H. S. (2000). "Functional expression and characterization of the two cyclic amidohydrolase enzymes, allantoinase and a novel phenylhydantoinase, from Escherichia coli." J Bacteriol 182:7021-7028. Pubmed: 11092864

D-phenylhydantoinase (Q46806)