UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (P77690)

Identification
Name:UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase
Synonyms:
  • Polymyxin resistance protein pmrH
  • UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase
  • UDP-Ara4O aminotransferase
Gene Name:arnB
Enzyme Class:
Biological Properties
General Function:Involved in transaminase activity
Specific Function:Catalyzes the conversion of UDP-4-keto-arabinose (UDP- Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Amino sugar and nucleotide sugar metabolism ec00520
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0L-Glutamate + 1.0UDP-4-Keto-pyranose ↔ 1.0alpha-Ketoglutarate + 1.0Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinose
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0L-Glutamic acid+1.0Thumb → 1.0Thumb+1.0Thumb
1.0UDP-β-L-threo-pentapyranos-4-ulose + 1.0L-Glutamic acid + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinose
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinose + 1.0Oxoglutaric acid ↔ 1.0UDP-4-Keto-pyranose + 1.0L-Glutamate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB214974-Amino-4-deoxy-L-arabinoseMetaboCard
ECMDB02812alpha-KetoglutarateMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB04123Oxoglutaric acidMetaboCard
ECMDB21293UDP-4-Keto-pyranoseMetaboCard
ECMDB23202UDP-beta-L-Threo-pentapyranos-4-uloseMetaboCard
ECMDB24240UDP-β-L-threo-pentapyranos-4-uloseMetaboCard
ECMDB21366Uridine 5''-diphospho-{beta}-4-deoxy-4-amino-L-arabinoseMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
pyridoxal phosphate binding
Gene Properties
Blattner:b2253
Gene OrientationClockwise
Centisome Percentage:50.95
Left Sequence End2363950
Right Sequence End2365089
Gene Sequence:
>1158 bp
ATGGCGGAAGGAAAAGCAATGTCAGAATTTTTGCCTTTTTCGCGACCAGCAATGGGCGTG
GAGGAACTCGCTGCAGTTAAAGAGGTTCTCGAATCCGGTTGGATCACAACCGGTCCGAAG
AATCAGGCGCTTGAGCAAGCTTTTTGCCAGTTGACGGGAAATCAGCATGCCATCGCGGTC
AGTTCAGCCACCGCCGGAATGCATATCACGCTAATGGCGTTGAAAATTGGCAAGGGCGAT
GAAGTGATTACGCCTTCCCTGACCTGGGTTTCAACCCTCAATATGATTTCCTTGTTGGGT
GCAACGCCGGTAATGGTGGATGTCGACCGCGATACGCTGATGGTCACGCCTGAAGCTATC
GAGTCAGCCATTACGCCACGCACTAAAGCCATCATTCCGGTGCATTATGCCGGTGCGCCA
GCAGATATTGACGCCATTCGCGCCATTGGCGAACGTTACGGCATCGCAGTTATCGAAGAT
GCTGCCCATGCCGTCGGTACGTATTACAAAGGGCGACATATTGGCGCAAAAGGTACCGCT
ATTTTTTCATTTCATGCCATTAAAAATATTACCTGTGCTGAAGGTGGCCTGATTGTAACT
GATAATGAAAACCTTGCCCGCCAGCTACGGATGCTGAAATTTCACGGTCTGGGTGTCGAT
GCCTATGACAGACAAACCTGGGGCCGTGCACCGCAGGCTGAAGTCTTAACACCGGGCTAT
AAGTACAATCTGACCGATATTAACGCCGCGATTGCCCTGACACAGTTAGTCAAATTAGAG
CACCTCAACACCCGTCGGCGCGAAATTGCCCAGCAATATCAGCAAGCACTGGCAGCTCTC
CCCTTTCAGCCATTAAGCCTTCCCGCCTGGCCGCACGTTCACGCCTGGCATCTGTTTATT
ATTCGTGTCGATGAACAACGTTGTGGTATCAGTCGCGATGCGTTGATGGAAGCGTTAAAA
GAAAGAGGCATTGGTACCGGGTTACATTTCCGCGCCGCTCACACACAAAAATATTATCGC
GAGCGTTTTCCCACGCTGTCGTTACCGAATACCGAATGGAATAGCGAACGCATCTGTTCT
TTGCCGCTGTTCCCGGATATGACTACCGCCGATGCCGACCATGTCATCACAGCCCTTCAG
CAACTCGCAGGACAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:379
Protein Molecular Weight:41649
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase
MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQLTGNQHAIAVSSATAG
MHITLMALKIGKGDEVITPSLTWVSTLNMISLLGATPVMVDVDRDTLMVTPEAIESAITP
RTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVGTYYKGRHIGAKGTAIFSFHA
IKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDRQTWGRAPQAEVLTPGYKYNLTD
INAAIALTQLVKLEHLNTRRREIAQQYQQALAALPFQPLSLPAWPHVHAWHLFIIRVDEQ
RCGISRDALMEALKERGIGTGLHFRAAHTQKYYRERFPTLSLPNTEWNSERICSLPLFPD
MTTADADHVITALQQLAGQ
References
External Links:
ResourceLink
Uniprot ID:P77690
Uniprot Name:ARNB_ECOLI
GenBank Gene ID:AF527386
Genebank Protein ID:22095313
Ecogene ID:EG14089
Ecocyc:EG14089
ColiBase:b2253
Kegg Gene:b2253
EchoBASE ID:EB3842
CCDB:ARNB_ECOLI
BacMap:145698288
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Breazeale, S. D., Ribeiro, A. A., Raetz, C. R. (2003). "Origin of lipid A species modified with 4-amino-4-deoxy-L-arabinose in polymyxin-resistant mutants of Escherichia coli. An aminotransferase (ArnB) that generates UDP-4-deoxyl-L-arabinose." J Biol Chem 278:24731-24739. Pubmed: 12704196
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837
  • Yan, A., Guan, Z., Raetz, C. R. (2007). "An undecaprenyl phosphate-aminoarabinose flippase required for polymyxin resistance in Escherichia coli." J Biol Chem 282:36077-36089. Pubmed: 17928292