ECMDB

Identification

Name:

N-hydroxyarylamine O-acetyltransferase

Synonyms:

Arylamine N-acetyltransferase
Arylhydroxamate N,O-acetyltransferase
NAT102

Gene Name:

nhoA

Enzyme Class:

2.3.1.118

Biological Properties

General Function:

Involved in acetyltransferase activity

Specific Function:

Acetyl-CoA + an N-hydroxyarylamine = CoA + an N-acetoxyarylamine

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

KEGG Reactions:

1.0

1.0N-Hydroxyarylamine

↔

1.0

1.0N-Acetoxyarylamine

1.0Acetyl-CoA + 1.0N-Hydroxyarylamine ↔ 1.0Coenzyme A + 1.0N-Acetoxyarylamine
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Acetyl-CoA + 1.02-Aminobenzoic acid → 1.0N-Acetylanthranilate + 1.0Coenzyme A
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB01123	2-Aminobenzoic acid	MetaboCard
ECMDB01206	Acetyl-CoA	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard
ECMDB21251	N-Acetylanthranilate	MetaboCard

GO Classification:

Function
acetyltransferase activity
acyltransferase activity
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
metabolic process

Gene Properties

Blattner:

b1463

Gene Orientation

Clockwise

Centisome Percentage:

33.02

Left Sequence End

1532048

Right Sequence End

1532893

Gene Sequence:

>846 bp
ATGAATAATTTACCGGTAGTTCGTTCGCCCTGGCGGATTGTGATTTTGCTGCTGGGCTTT
ACCTTTCTCTACGCGCCAATGCTGATGCTGGTTATCTATTCGTTTAACAGCTCGAAGCTG
GTGACGGTGTGGGCCGGCTGGTCAACGCGCTGGTATGGTGAGTTATTGCGCGATGACGCG
ATGATGAGTGCGGTTGGTTTAAGTCTGACAATTGCGGCCTGTGCGGCGACGGCGGCGGCG
ATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAAT
GGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCG
CTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATG
CTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCT
TCAAGATTGCGGGAACTGGATCGCTCGATAGAAGAAGCAGCGATGGATCTCGGTGCGACG
CCGCTGAAAGTGTTTTTCGTCATTACGCTACCGATGATCATGCCCGCGATCATTTCTGGC
TGGTTACTGGCTTTTACTTTGTCGCTTGATGATCTGGTGATCGCCAGCTTTGTTTCCGGG
CCGGGAGCCACCACGTTACCGATGCTGGTCTTTTCCAGCGTGCGGATGGGGGTGAATCCG
GAAATCAACGCCCTGGCAACATTGATCCTCGGCGCGGTCGGAATTGTCGGATTTATCGCC
TGGTATCTGATGGCTCGCGCAGAAAAACAGCGGATACGCGACATCCAGCGTGCAAGACGT
GGCTGA

Protein Properties

Pfam Domain Function:

Acetyltransf_2 (PF00797 )

Protein Residues:

281

Protein Molecular Weight:

32274

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>N-hydroxyarylamine O-acetyltransferase
MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVLLPREIQLDNQSPEEKL
VIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPALPPRTHRLLLVELEEEKWI
ADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVLQFNHHQHWQSMYRFDLCEQQ
QSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLTNFHFTHYENGHAVEQRNLPDVA
SLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTHPEAGK

References

External Links:

Resource	Link
Uniprot ID:	P77567
Uniprot Name:	NHOA_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1651390
Ecogene ID:	EG13780
Ecocyc:	EG13780
ColiBase:	b1463
Kegg Gene:	b1463
EchoBASE ID:	EB3542
CCDB:	NHOA_ECOLI
BacMap:	16129422

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553

N-hydroxyarylamine O-acetyltransferase (P77567)