Sensor histidine kinase DpiB (P77510)

Identification
Name:Sensor histidine kinase DpiB
Synonyms:
  • Sensor histidine kinase CitA
Gene Name:dpiB
Enzyme Class:
Biological Properties
General Function:Signal transduction mechanisms
Specific Function:Member of the two-component regulatory system DpiA/DpiB, which is essential for expression of citrate-specific fermentation genes and genes involved in plasmid inheritance. Could be involved in response to both the presence of citrate and external redox conditions. Functions as a sensor kinase that phosphorylates DpiA in the presence of citrate
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
GO Classification:
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
molecular transducer activity
nucleoside binding
protein histidine kinase activity
protein kinase activity
purine nucleoside binding
signal transducer activity
transferase activity
transferase activity, transferring phosphorus-containing groups
two-component sensor activity
Process
biological regulation
cellular metabolic process
metabolic process
peptidyl-histidine phosphorylation
phosphate metabolic process
phosphorus metabolic process
phosphorylation
protein amino acid phosphorylation
regulation of biological process
regulation of cellular process
regulation of gene expression
regulation of macromolecule metabolic process
regulation of metabolic process
regulation of transcription
regulation of transcription, DNA-dependent
signal transduction
signal transmission
signaling process
two-component signal transduction system (phosphorelay)
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1659 bp
ATGTTGCAGCTTAACGAGAATAAACAGTTTGCATTTTTCCAAAGACTGGCATTTCCGCTG
CGTATCTTTTTGCTGATTCTGGTGTTCTCAATATTTGTCATTGCAGCCCTGGCGCAATAT
TTTACGGCCAGTTTTGAGGACTATTTAACGCTTCATGTACGCGACATGGCAATGAATCAG
GCGAAAATTATTGCCTCCAATGACAGTGTCATCTCTGCGGTGAAAACGCGTGACTACAAA
CGGCTGGCGACCATCGCTAACAAATTACAAAGAGATACCGATTTTGATTATGTGGTGATT
GGGGACCGGCACTCGATCCGCCTTTACCATCCTAATCCGGAGAAAATTGGTTATCCTATG
CAGTTCACCAAACAGGGCGCGCTGGAGAAAGGGGAGAGCTACTTCATTACCGGGAAAGGG
TCAATGGGGATGGCGATGCGCGCCAAAACGCCAATCTTTGATGACGATGGAAAAGTCATC
GGCGTGGTGTCGATTGGCTACCTGGTGAGTAAAATCGATAGCTGGCGGGCTGAGTTTTTA
TTACCGATGGCAGGTGTGTTTGTCGTGCTGTTAGGGATTCTGATGTTGCTGTCGTGGTTC
CTGGCCGCGCATATCCGTCGGCAGATGATGGGCATGGAGCCAAAGCAAATCGCACGCGTG
GTCCGTCAGCAAGAGGCGCTGTTTAGTTCGGTTTATGAAGGGCTGATTGCGGTGGATCCG
CATGGTTACATTACCGCCATCAATCGTAACGCAAGAAAGATGCTGGGGCTGAGTTCCCCC
GGACGGCAATGGTTGGGTAAACCCATTGTTGAAGTGGTCAGGCCCGCCGATTTCTTTACC
GAACAGATTGATGAAAAACGTCAGGATGTGGTGGCGAACTTTAACGGTCTGAGCGTTATT
GCCAACCGGGAAGCTATTCGTTCAGGTGATGATTTGCTGGGGGCCATTATCAGCTTTCGT
AGTAAAGACGAAATTTCCACCCTCAATGCGCAACTGACGCAAATAAAACAATACGTTGAG
AGCCTTCGTACATTGCGACACGAGCATCTCAATTGGATGTCGACGCTCAATGGTCTGTTG
CAGATGAAAGAGTATGATCGCGTGCTGGCGATGGTGCAGGGGGAGTCTCAGGCCCAGCAA
CAGCTTATTGACAGCCTGCGCGAGGCGTTTGCCGATCGCCAGGTGGCGGGGCTGCTTTTT
GGTAAAGTGCAGCGCGCCCGGGAACTGGGGCTAAAAATGATCATTGTCCCCGGTAGCCAG
CTTTCGCAACTGCCGCCAGGACTGGATAGCACCGAGTTTGCAGCCATTGTGGGCAATTTA
CTTGATAACGCCTTCGAAGCCAGCCTGCGTAGCGATGAAGGAAACAAGATCGTTGAATTA
TTCCTCAGCGATGAAGGCGATGATGTGGTGATTGAAGTCGCCGATCAGGGCTGCGGCGTT
CCAGAGTCTCTACGAGACAAAATATTTGAGCAGGGGGTCAGTACGCGTGCTGACGAGCCC
GGTGAACATGGCATTGGGTTGTACTTGATTGCCAGCTACGTAACGCGCTGCGGTGGTGTT
ATCACTCTCGAAGATAATGATCCCTGCGGTACCTTATTTTCAATCTATATTCCGAAAGTG
AAACCTAATGACAGCTCCATTAACCCTATTGATCGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:552
Protein Molecular Weight:61684
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • 22-42
  • 183-203
Protein Sequence:
>Sensor histidine kinase DpiB
MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTASFEDYLTLHVRDMAMNQ
AKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGDRHSIRLYHPNPEKIGYPM
QFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVIGVVSIGYLVSKIDSWRAEFL
LPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIARVVRQQEALFSSVYEGLIAVDP
HGYITAINRNARKMLGLSSPGRQWLGKPIVEVVRPADFFTEQIDEKRQDVVANFNGLSVI
ANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQIKQYVESLRTLRHEHLNWMSTLNGLL
QMKEYDRVLAMVQGESQAQQQLIDSLREAFADRQVAGLLFGKVQRARELGLKMIIVPGSQ
LSQLPPGLDSTEFAAIVGNLLDNAFEASLRSDEGNKIVELFLSDEGDDVVIEVADQGCGV
PESLRDKIFEQGVSTRADEPGEHGIGLYLIASYVTRCGGVITLEDNDPCGTLFSIYIPKV
KPNDSSINPIDR
References
External Links:
ResourceLink
Uniprot ID:P77510
Uniprot Name:DPIB_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1786837
CCDB:DPIB_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ingmer, H., Miller, C. A., Cohen, S. N. (1998). "Destabilized inheritance of pSC101 and other Escherichia coli plasmids by DpiA, a novel two-component system regulator." Mol Microbiol 29:49-59. Pubmed: 9701802
  • Kaspar, S., Bott, M. (2002). "The sensor kinase CitA (DpiB) of Escherichia coli functions as a high-affinity citrate receptor." Arch Microbiol 177:313-321. Pubmed: 11889485
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Yamamoto, K., Matsumoto, F., Minagawa, S., Oshima, T., Fujita, N., Ogasawara, N., Ishihama, A. (2009). "Characterization of CitA-CitB signal transduction activating genes involved in anaerobic citrate catabolism in Escherichia coli." Biosci Biotechnol Biochem 73:346-350. Pubmed: 19202292