2-methylcitrate dehydratase (P77243)

Identification
Name:2-methylcitrate dehydratase
Synonyms:Not Available
Gene Name:prpD
Enzyme Class:
Biological Properties
General Function:Involved in 2-methylcitrate dehydratase activity
Specific Function:Catalyzes the dehydration of 2-methylcitrate to 2- methyl-cis-aconitate. Also seems to be responsible for the residual aconitase activity of the acnAB-null strain
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Methylcitric acid + 1.0(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate ↔ 1.0Cis-2-Methylaconitate + 1.0Water
ReactionCard
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Methylcitric acid ↔ 1.0Cis-2-Methylaconitate + 1.0Water
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Methylcitric acid → 1.0Water + 1.0Cis-2-Methylaconitate
ReactionCard
SMPDB Reactions:
1.02-Methylcitric acid+1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Methylcitric acid + 1.0Methylcitric acid → 1.0Water + 1.0Cis-2-Methylaconitate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Methylcitric acid + 1.0(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate ↔ 1.0Cis-2-Methylaconitate + 1.0Water
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Methylcitric acid → 1.0Water + 1.0Cis-2-Methylaconitate
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.0(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate → 1.0Cis-2-Methylaconitate + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23051(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylateMetaboCard
ECMDB06357Cis-2-MethylaconitateMetaboCard
ECMDB00723Methylcitric acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
2 iron, 2 sulfur cluster binding
2-methylcitrate dehydratase activity
binding
carbon-oxygen lyase activity
catalytic activity
hydro-lyase activity
iron-sulfur cluster binding
lyase activity
metal cluster binding
Process
carboxylic acid metabolic process
cellular metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
propionate catabolic process
propionate catabolic process, 2-methylcitrate cycle
propionate metabolic process
Gene Properties
Blattner:b0334
Gene OrientationClockwise
Centisome Percentage:7.55
Left Sequence End350439
Right Sequence End351890
Gene Sequence:
>1452 bp
ATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAATCGTTGATATCGTC
GATTACGTCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGC
CTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTG
CTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAG
TTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGAT
TTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATT
CTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACC
ATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTG
GAAAACTCCTTTAACCGCGTCGGCCTCGACCACGTTCTGTTAGTGAAAGTGGCTTCCACC
GCCGTGGTCGCCGAAATGCTCGGCCTGACCCGCGAGGAAATTCTCAACGCCGTTTCGCTG
GCGTGGGTGGACGGTCAGTCGCTGCGCACCTATCGCCATGCGCCGAACACCGGCACGCGT
AAATCCTGGGCGGCGGGCGATGCCACTTCCCGCGCGGTACGTCTGGCACTGATGGCGAAA
ACGGGCGAAATGGGTTACCCGTCAGCCCTGACTGCGCCGGTGTGGGGCTTCTACGACGTC
TCCTTTAAAGGTGAATCGTTCCGCTTCCAGCGCCCGTACGGTTCCTACGTTATGGAAAAT
GTGCTGTTCAAAATCTCCTTCCCGGCGGAGTTCCACTCCCAGACGGCAGTTGAAGCAGCG
ATGACGCTCTATGAACAGATGCAGGCAGCAGGCAAAACGGCGGCGGATATCGAAAAAGTG
ACCATTCGCACCCACGAAGCCTGTATTCGCATCATCGACAAAAAAGGGCCGCTCAATAAC
CCGGCAGACCGCGATCACTGCATTCAGTACATGGTGGCGATCCCGCTGCTATTCGGGCGC
TTAACGGCGGCAGATTACGAGGACAACGTTGCGCAAGATAAACGCATTGACGCCCTGCGC
GAGAAGATCAATTGCTTTGAAGATCCGGCATTTACCGCTGACTACCACGACCCGGAAAAA
CGCGCCATCGCCAATGCCATTACCCTTGAGTTCACCGACGGCACACGATTTGAAGAAGTG
GTGGTGGAGTACCCCATTGGTCATGCTCGCCGCCGTCAGGATGGTATTCCGAAACTGGTC
GATAAATTCAAAATCAATCTCGCGCGCCAGTTCCCGACTCGCCAACAGCAGCGCATTCTG
GAGGTTTCTCTCGACAGAGCTCGCCTGGAACAGATGCCGGTCAATGAGTATCTCGACCTG
TACGTCATTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:483
Protein Molecular Weight:53951
Protein Theoretical pI:6
PDB File:1SZQ
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-methylcitrate dehydratase
MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDTLGCGLEALEYPACKKL
LGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGI
LATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIALENSFNRVGLDHVLLVKVAST
AVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTGTRKSWAAGDATSRAVRLALMAK
TGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSYVMENVLFKISFPAEFHSQTAVEAA
MTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDKKGPLNNPADRDHCIQYMVAIPLLFGR
LTAADYEDNVAQDKRIDALREKINCFEDPAFTADYHDPEKRAIANAITLEFTDGTRFEEV
VVEYPIGHARRRQDGIPKLVDKFKINLARQFPTRQQQRILEVSLDRARLEQMPVNEYLDL
YVI
References
External Links:
ResourceLink
Uniprot ID:P77243
Uniprot Name:PRPD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674476
PDB ID:1SZQ
Ecogene ID:EG13603
Ecocyc:EG13603
ColiBase:b0334
Kegg Gene:b0334
EchoBASE ID:EB3371
CCDB:PRPD_ECOLI
BacMap:16128319
General Reference:
  • Blank, L., Green, J., Guest, J. R. (2002). "AcnC of Escherichia coli is a 2-methylcitrate dehydratase (PrpD) that can use citrate and isocitrate as substrates." Microbiology 148:133-146. Pubmed: 11782506
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553